Resveratrol modifies biliary secretion of cholephilic compounds in sham-operated and cholestatic rats

Table 1 Pre-designed TaqMan^® gene expression assay kits (Life Technologies) used for quantitative real-time RT-PCR

Gene symbol	Transporter/Receptor	Life technologies cat. number
Abcc2	Mrp2	Rn00563231_m1
Abcb11	Bsep	Rn00582179_m1
Abcg2	Bcrp	Rn00710585_m1
Abcb1a	Mdr1a	Rn00591394_ml
Abcb1b	Mdr1b	Rn00561753_ml
Abcb4	Mdr2	Rn00562185_ml
Abcc3	Mrp3	Rn01452854_m1
Abcc4	Mrp4	Rn01465702_m1
Slc10a1	Ntcp	Rn00566894_ml
Slco1a1	Oatp1a1	Rn00755148_m1
Slco1a4	Oatp1a4	Rn00756233_ml
Slc22a7	Oat2	Rn00585513_m1
Slc10a2	Asbt	Rn00691576_m1
Cyp7a1		Rn00564065_m1
Cyp8b1		Rn00579921_m1
NrOb2	Shp	Rn00589173_m1
TNF-α		Rn99999017_m1
IL-6		Rn99999011_ml
Acta2		Rn01759928_g1
TGF-β1		Rn00572010_m1
Col1a1		Rn01463848_m1
Pdgfrβ		Rn00709573_m1
Timp-2		Rn00573232_m1
Nqo1		Rn00566528_m1
GAPDH		4352338E

Table 2 Primary and secondary antibodies used in western blot analysis

Protein	Source	Dilution	Secondary antibody dilution
Mrp2	Signet Laboratories	1:500	1:1000
Bsep	Santa Cruz	1:300	1:2000
Bcrp	Signet Laboratories	1:500	1:500
P-gp	Signet Laboratories	1:500	1:1000
Mdr2	Abcam	1:500	1:500
Mrp3	Alexis	1:500	1:500
Mrp4	Abcam	1:1000	1:1000
Ntcp	Santa Cruz	1:300	1:3000
Oatp1a4	Millipore	1:5000	1:5000
Cyp7a1	Thermo Fisher Scientific	1:1000	1:2000
Cyp8b1	Thermo Fisher Scientific	1:500	1:1000
Sirt1	Cell Signaling	1:1000	1:2000
p-AMPKα	Cell Signaling	1:1000	1:2000
PCNA	Sigma-Aldrich	1:800	1:2000
ZO-1	Invitrogen	1:500	1:1000
β-actin	Sigma	1:5000	1:5000