MicroRNA profile in neosquamous esophageal mucosa following ablation of Barrett’s esophagus

Table 1 Fold differences in gene expression and Mann Whitney U test P values for the microRNAs that were differentially expressed in both post-NS vs control-S and post-NS vs post-S mucosa comparisons

Mature miRNA	Post-NS/control-S	P value	Post-NS/ Post-S	P value	Higher in pre-BE vs post-NS
miR-424-5p	485.2	0.00002	233.7	0.00053	Yes
miR-135b-5p	2.0	0.00071	1.7	0.00363	No
miR-376c-3p	6.4	0.00145	3.1	0.02673	Yes
miR-135a-5p	3.0	0.00224	2.5	0.00821	Yes
miR-187-3p	208.1	0.00414	2.7	0.01196	No
miR-409-3p	6.8	0.00502	4.6	0.00272	Yes
miR-214-5p	47.0	0.00502	46.1	0.02673	Yes
miR-31-5p	1.44	0.00869	1.5	0.00632	No
miR-199a-5p	396.3	0.01223	512.9	0.04478	Yes
miR-223-5p	230.9	0.02306	204.5	0.01350	Yes
miR-127-3p	4.8	0.02675	3.8	0.03305	Yes
miR-136-3p	7.2	0.02675	201.2	0.03305	Yes

Table 2 Predicted molecular pathways of the mRNA targets of differentially expressed microRNAs that are increased in neosquamous but not Barrett’s esophagus mucosa

Pathway name	Pathway uploaded gene count	Genes in InnateDB for this entity	Pathway P value	Pathway P value (corrected)
Ion transport by P-type ATPases	5	43	1.57E-05	0.005
Transmembrane transport of small molecules	14	606	1.31E-04	0.022
Ion channel transport	7	169	2.56E-04	0.028
Calcium signaling pathway	7	183	4.15E-04	0.035

Predicted molecular pathways using miRWalk to generate predicted mRNA targets of the microRNAs differentially expressed in both Neosquamous vs healthy control and Neosquamous vs Proximal squamous comparisons, and InnateDB to identify pathways in which the mRNA targets are over-represented.

Table 3 Predicted molecular pathways of the mRNA targets of differentially expressed microRNAs that are increased in both neosquamous and Barrett’s esophagus mucosa

Pathway name	Pathway uploaded gene count	Genes in InnateDB for this entity	Pathway P value	Pathway P value (corrected)
JAK STAT pathway and regulation	28	273	5.41E-06	0.005
Hemostasis	41	508	1.68E-05	0.005
Regulation of bad phosphorylation	7	23	2.36E-05	0.006