Polymorphisms of microRNA target genes IL12B, INSR, CCND1 and IL10 in gastric cancer

Table 1 Selected target genes and their corresponding single-nucleotide polymorphisms

Chromosome	Target gene	miRNA	Delta S	SNP ID	Position	MAF
5	IL12B	miR-27	0.3249	rs1368439	15874204	0.202
19	INSR	miR-146a	0.2591	rs1051690	7116963	0.242
11	CCDN1	miR-223	0.4614	rs7177	69466115	0.419
1	IL10	miR-107	0.7057	rs3024498	206941529	0.267

SNP ID: Single-nucleotide polymorphisms number; MAF: Minor allele frequency.

Table 2 Characteristics of gastric cancer patients and control subjects n (%)

	Controls (n = 508)	Gastric cancer patients (n = 474)	P value
Age (mean ± SD)	58.1 ± 17. 4	62.5 ± 18. 4	< 0.0011
Gender
Male	139 (27.4)	288 (60.8)	< 0.0012
Female	366 (72.0)	178 (37.5)
Unknown	3 (0.6)	8 (1.7)
Country of birth
Latvia	146 (28.7)	139 (29.3)	0.8662
Lithuania	258 (50.8)	233 (49.2)
Germany	104 (20.5)	102 (21.5)

¹Student t-test;

²χ² test.

Table 3 Genotype and allele frequencies in control and gastric cancer patients and odds ratio of gastric cancer by genotypes n (%)

Genotype	Controls (n = 508)	Gastric cancer patients (n = 474)	OR	95%CI	P value
IL12B (rs1368439)
TT	366 (72.05)	338 (71.31)	1.00
TG	137 (26.97)	127 (26.79)	0.87	0.63-1.18	0.39
GG	5 (0.98)	9 (1.90)	1.27	0.39-4.14	0.68
GG vs TG + TT			1.33	0.41-4.30	0.63
GG + TG vs TT			0.88	0.65-1.20	0.42
Allele T	869 (85.53)	803 (84.70)	1.00	0.83-1.45	0.63
Allele G	147 (14.47)	145 (15.30)	1.10
INSR (1051690)
CC	334 (65.75)	272 (57.51)	1.00
CT	153 (30.12)	182 (38.48)	1.45	1.08-1.95	0.01
TT	21 (4.13)	19 (4.02)	1.30	0.66-2.60	0.44
TT vs CT + CC			1.15	0.58-2.30	0.70
TT + CT vs CC			1.44	1.08-1.90	0.01
Allele C	821 (80.81)	726 (76.74)	1.00
Allele T	195 (19.19)	220 (23.26)	1.32	1.04-1.67	0.02
CCND1 (rs7177)
AA	160 (31.56)	159 (33.62)	1.00
AC	245 (48.32)	220 (46.51)	0.92	0.68-1.26	0.63
CC	102 (20.12)	94 (19.87)	1.07	0.73-1.58	0.70
CC vs AC + AA			1.12	0.80-1.60	0.50
CC + AC vs AA			0.97	0.72-1.30	0.83
Allele A	565 (55.72)	538 (56.87)	1.00
Allele C	449 (44.28)	408 (43.13)	1.02	0.84-1.24	0.81
IL10 (rs3024498)
TT	252 (50.30)	259 (54.87)	1.00
TC	217 (43.31)	185 (39.19)	0.92	0.69-1.22	0.57
CC	32 (6.39)	28 (5.93)	1.05	0.58-1.87	0.88
CC vs TC + TT			1.08	0.29-1.61	0.78
CC + TC vs TT			0.94	0.71-1.23	0.64
Allele T	721 (71.96)	703 (74.47)	1.00
Allele C	281 (28.04)	241 (25.53)	1.03	0.83-1.30	0.78