Aging related methylation influences the gene expression of key control genes in colorectal cancer and adenoma

Table 1 Clinical data of samples involved in the high resolution melting study

Sample ID	Age	Gender	Localization	Histology	TNM	Grade	Dukes’ stage (MAC)	Dysplasia	Adenoma size	Sample type
Ch1	4	M	Colon	Normal						FFPE
Ch2	7	F	Cecum	Normal						FFPE
Ch3	11	M	Colon	Normal						FFPE
Ch4	14	M	Transverse	Normal						FFPE
Ch5	5	F	Sigmoid	Normal						FFPE
Ch6	7	F	Descendent	Normal						FFPE
Ch7	1	M	Descendent	Normal						FFPE
Ch8	1	M	Sigmoid	Normal						FFPE
Ch9	10	M	Cecum	Normal						FFPE
Ch10	3	M	Sigmoid	Normal						FFPE
Ch11	17	F	Cecum	Normal						FFPE
Ch12	17	F	Sigmoid	Normal						FFPE
Ch13	16	F	Sigmoid	Normal						FFPE
Ch14	16	F	Cecum	Normal						FFPE
Ch15	1	M	Left colon	Normal						FF
Ch16	3	F	Sigmoid	Normal						FF
Ch17	6	M	Sigmoid	Normal						FF
Ch18	9	M	Sigmoid	Normal						FF
Ch19	17	M	Colon	Normal						FF
N1	44	F	Sigmoid	Normal						FF
N2	31	F	Sigmoid	Normal						FF
N3	59	F	Sigmoid	Normal						FF
N4	54	M	Colon	Normal						FF
N5	68	F	Sigmoid	Normal						FF
N6	71	F	Sigmoid	Normal						FF
N7	69	F	Sigmoid	Normal						FF
N8	57	F	Sigmoid	Normal						FF
AD1	78	M	Asc, sigmoid, rectum	Tubulovillous adenoma				Low-grade	30 mm, 3 mm, 15 mm	FF
AD2	60	M	Sigmoid	Tubular adenoma				Low-grade	6 mm	FF
AD3	88	M	Asc, transv, sigmoid	Tubular adenoma				Low-grade	4 mm, 3 mm, 7-8 mm	FF
AD4	72	F	Rectum	Tubular adenoma				Low-grade	10 mm	FF
AD5	45	F	Descendent	Tubular adenoma				High-grade	5-6 mm	FF
AD6	68	F	Rectum	Tubular adenoma				Low-grade	5 mm	FF
AD7	63	F	Sigmoid	Tubular adenoma				Low-grade	8 mm	FF
AD8	65	F	Asc, transv, rectum	Tubular adenoma				Low-grade	2-3 mm, 2-3 mm, 2-3 mm	FF
AD9	60	F	Sigmoid	Tubular adenoma				Low-grade	5 mm, 4 mm	FF
AD10	77	F	Rectosigmoid	Tubular adenoma				Low-grade	5 mm	FF
AD11	55	F	Asc colon	Tubular adenoma				Low-grade	10 mm	FF
AD12	76	M	Cecum, sigmoid	Tubular adenoma				Low-grade	5 mm, 8-10 mm	FF
AD13	62	F	Sigmoid	Tubular adenoma				High-grade	30 mm	FF
AD14	83	M	Asc colon	Tubulovillous adenoma				High-grade	50-60 mm	FF
AD15	73	M	Cecum, asc, desc	Tubular adenoma				Low-grade	12 mm, 10 mm, 6-8 mm	FF
AD16	64	M	Transv, sigmoid, rectum	Tubular adenoma				Low-grade	5 mm, 25 mm, 15 mm	FF
AD17	63	M	Asc, transv, rectum	Tubular adenoma				Low-grade	2-3 mm, 5 mm, 5-6 mm, 15 mm	FF
AD18	63	F	Sigmoid	Tubulovillous adenoma				Low-grade	25 mm	FF
AD19	63	M	Rectum	Tubulovillous and tubular adenoma				Low-grade	25 mm, 30 mm	FF
AD20	87	M	Sigmoid, rectum	Tubulovillous adenoma				Low-grade	20 mm, 15 mm	FF
AD21	63	F	Sigmoid	Tubulovillous adenoma				Low-grade	25 mm	FF
CRC1	67	F	Sigmoid	Adenocarcinoma	Unknown	Unknown	Unknown			FF
CRC2	76	F	Hepatic flexure	Adenocarcinoma	T2N0M0	2	B1			FF
CRC3	73	F	Sigmoid	Adenocarcinoma	T3N2M1	2	D			FF
CRC4	65	M	Sigmoid	Adenocarcinoma	T2N0M0	1	B1			FF
CRC5	85	F	Cecum	Adenocarcinoma	T2N0M0	2	B1			FF
CRC6	60	M	Hepatic flexure	Adenocarcinoma		unknown	D			FF
CRC7	68	M	Sigmoid, rectum	Adenocarcinoma	T3N0M0	1	B2			FF
CRC8	87	F	Sigmoid	Adenocarcinoma	T3N0M0	2	B2			FF

Ch: Children; N: Normal; Ad: Adenoma; CRC: Colorectal cancer.

Table 2 Significant DNA methylation alterations of age-related CpG sites in CRC samples compared to normal tissue

cgID	Gene symbol	P value	Δβ (CRC - N)
cg06462291	NT5DC3	3.81 × 10-6	-0.27
cg06144905	PIPOX	1.81 × 10-7	-0.22
cg10345936	SLC36A2	2.49 × 10-5	-0.18
cg13828047	MPI	1.78 × 10-5	-0.17
cg11314684	AKT3	4.55 × 10-5	-0.16
cg00431549	MGP	0.020	-0.12
cg14409958	ENPP2	0.021	-0.11
cg00091693	KRT20	0.010	-0.11
cg17274064	ERG	1.48 × 10-4	-0.11
cg22809047	RPL31	1.91 × 10-3	-0.10
cg00075967	STRA6	0.010	-0.10
cg06952310	NCAN	7.13 × 10-3	-0.10
cg02388150	SFRP1	3.33 × 10-3	0.10
cg03588357	GPR68	7.72 × 10-3	0.10
cg26297688	TMEM263	6.21 × 10-3	0.10
cg04528819	KLF14	0.036	0.10
cg26372517	TFAP2E	9.23 × 10-3	0.11
cg08030082	POMC	1.93 × 10-3	0.11
cg05675373	KCNC4	7.80 × 10-3	0.11
cg10281002	TBX5	0.012	0.11
cg06117855	CLEC3B	1.87 × 10-3	0.11
cg09509673	CCR10	1.91 × 10-4	0.11
cg14597908	GNAS	1.48 × 10-4	0.11
cg27494383	LTK	9.32 × 10-3	0.12
cg21870884	GPR25	5.77 × 10-3	0.13
cg25657834	NTSR2	4.43 × 10-3	0.13
cg22449114	TCF15	3.48 × 10-5	0.13
cg04126866	C10orf99	4.52 × 10-4	0.13
cg25552492	LGI3	9.44 × 10-3	0.14
cg08965235	LTBP3	3.12 × 10-3	0.14
cg06836772	PRKAA2	0.013	0.14
cg02364642	ARHGEF25	1.20 × 10-3	0.16
cg18573383	KCNC2	1.38 × 10-3	0.16
cg12616277	ESYT3	0.015	0.16
cg17729667	NINL	3.46 × 10-3	0.16
cg04999691	ZBED6CL	3.32 × 10-11	0.16
cg12373771	CECR6	3.70 × 10-3	0.17
cg02489552	CCDC105	8.91 × 10-8	0.17
cg25148589	GRIA2	7.77 × 10-6	0.19
cg21096399	MCAM	5.47 × 10-6	0.19
cg20914508	GAP43	4.24 × 10-4	0.20
cg12768605	LYPD5	1.85 × 10-4	0.21
cg13216057	DKK3	2.10 × 10-4	0.21
cg12351433	LHCGR	4.97 × 10-7	0.22
cg08434234	DGKI	5.16 × 10-5	0.23
cg10920957	JPH3	3.00 × 10-4	0.26
cg27092035	ARL10	3.73 × 10-5	0.27
cg06557358	TMEM132E	3.17 × 10-6	0.27
cg26620959	SYNE1	1.32 × 10-6	0.30
cg07663789	NPR3	8.73 × 10-6	0.31
cg09191327	PRDM12	1.79 × 10-6	0.31
cg25070637	SDC2	1.64 × 10-6	0.34
cg10486998	GALR1	1.81 × 10-8	0.35
cg24834740	PPP1R16B	3.56 × 10-12	0.37
cg27319898	ZNF804B	5.41 × 10-8	0.38
cg08090772	ADHFE1	7.18 × 10-14	0.39
cg02217159	KHDRBS2	6.67 × 10-13	0.43

N: Normal; CRC: Colorectal cancer.

Table 3 Significant DNA methylation changes of age-related CpG sites in adenoma samples compared to normal tissue

cgID	Gene symbol	P value	Δβ (AD - N)
cg10345936	SLC36A2	3.49 × 10-10	-0.34
cg00091693	KRT20	2.49 × 10-12	-0.30
cg00075967	STRA6	2.58 × 10-9	-0.26
cg11314684	AKT3	1.03 × 10-9	-0.26
cg06462291	NT5DC3	7.45 × 10-5	-0.25
cg17099569	GLI2	1.25 × 10-10	-0.23
cg00168942	GJD4	2.48 × 10-7	-0.20
cg20828084	CEMIP	1.33 × 10-9	-0.19
cg17274064	ERG	1.82 × 10-10	-0.19
cg07408456	PGLYRP2	2.41 × 10-12	-0.19
cg03019000	TEX264	2.25 × 10-7	-0.19
cg17589341	SLC14A1	1.42 × 10-9	-0.19
cg02580606	KRT33B	9.08 × 10-5	-0.17
cg00436603	CYP2E1	1.37 × 10-8	-0.16
cg19346193	UROS	6.59 × 10-7	-0.15
cg03103192	SPATA18	1.39 × 10-7	-0.15
cg25564800	KPNA1	2.18 × 10-8	-0.14
cg06144905	PIPOX	3.63 × 10-7	-0.14
cg06952310	NCAN	2.72 × 10-4	-0.14
cg13038560	C2orf47	2.13 × 10-5	-0.13
cg22190114	NLRP8	1.67 × 10-4	-0.13
cg13302154	MGP	7.27 × 10-6	-0.13
cg01262913	DSCR9	2.67 × 10-6	-0.13
cg14258236	OR5V1	3.71 × 10-4	-0.12
cg13828047	MPI	1.01 × 10-3	-0.12
cg01459453	SELP	1.65 × 10-4	-0.12
cg14894144	LAMA3	2.29 × 10-4	-0.12
cg07337598	ANXA9	2.02 × 10-5	-0.12
cg03270204	DDR1	7.47 × 10-5	-0.12
cg12946225	HMG20B	1.37 × 10-6	-0.11
cg09646392	TNFSF13B	2.66 × 10-5	-0.10
cg19305227	SLC28A2	0.033	-0.10
cg03578041	LARP6	2.22 × 10-5	-0.10
cg07455279	NDUFA3	0.013	-0.10
cg13899108	PDE4C	5.00 × 10-6	0.10
cg04999691	ZBED6CL	4.92 × 10-5	0.10
cg14597908	GNAS	9.38 × 10-5	0.10
cg21870884	GPR25	9.49 × 10-3	0.11
cg12616277	ESYT3	0.022	0.12
cg12373771	CECR6	3.55 × 10-3	0.12
cg17729667	NINL	4.43 × 10-3	0.13
cg02364642	ARHGEF25	2.33 × 10-3	0.14
cg22449114	TCF15	2.39 × 10-5	0.14
cg03565323	ZNF287	4.52 × 10-3	0.14
cg02388150	SFRP1	1.20 × 10-7	0.15
cg12768605	LO×L2	8.01 × 10-3	0.15
cg25657834	NTSR2	1.46 × 10-4	0.16
cg20914508	GAP43	9.41 × 10-4	0.16
cg10281002	TBX5	1.26 × 10-5	0.17
cg02489552	CCDC105	7.16 × 10-8	0.18
cg05675373	KCNC4	1.82 × 10-6	0.18
cg25148589	GRIA2	6.11 × 10-7	0.18
cg13216057	DKK3	9.64 × 10-4	0.18
cg27092035	ARL10	8.81 × 10-4	0.19
cg12351433	LHCGR	2.20 × 10-6	0.20
cg18573383	KCNC2	5.43 × 10-7	0.21
cg08434234	DGKI	6.60 × 10-5	0.22
cg21096399	MCAM	5.20 × 10-8	0.22
cg10920957	JPH3	1.03 × 10-4	0.25
cg08965235	LTBP3	6.03 × 10-10	0.25
cg27319898	ZNF804B	5.48 × 10-5	0.26
cg26620959	SYNE1	3.89 × 10-6	0.29
cg25070637	SDC2	1.82 × 10-5	0.30
cg07663789	NPR3	6.41 × 10-7	0.31
cg09191327	PRDM12	4.08 × 10-7	0.31
cg06557358	TMEM132E	2.04 × 10-8	0.33
cg10486998	GALR1	2.61 × 10-9	0.36
cg24834740	PPP1R16B	2.23 × 10-9	0.36
cg08090772	ADHFE1	5.19 × 10-11	0.39
cg02217159	KHDRBS2	1.87 × 10-11	0.41

N: Normal; Ad: Adenoma.

Table 4 Significant DNA methylation alterations of age-related CpG sites in colorectal cancer samples compared to adenoma tissue

cgID	Gene symbol	P value	Δβ (CRC - AD)
cg08965235	LTBP3	9.09 × 10-4	-0.11
cg00945507	SEC61G	1.64 × 10-5	0.10
cg15974053	HSD17B14	2.27 × 10-3	0.10
cg24262469	TIPARP	5.33 × 10-8	0.10
cg07158339	FXN	1.84 × 10-7	0.10
cg11314684	AKT3	4.52 × 10-4	0.10
cg03578041	LARP6	2.57 × 10-9	0.10
cg17853587	NDST3	5.94 × 10-4	0.10
cg07408456	PGLYRP2	1.51 × 10-7	0.11
cg02580606	KRT33B	1.22 × 10-4	0.11
cg03019000	TEX264	5.39 × 10-5	0.11
cg27319898	ZNF804B	0.023	0.12
cg00436603	CYP2E1	1.97 × 10-6	0.12
cg15804973	MAP3K5	6.27 × 10-12	0.12
cg06117855	CLEC3B	1.73 × 10-7	0.13
cg24126851	DCHS1	3.58 × 10-8	0.13
cg16034652	UNC79	4.23 × 10-11	0.13
cg01262913	DSCR9	1.64 × 10-8	0.13
cg26372517	TFAP2E	9.80 × 10-6	0.13
cg03270204	DDR1	4.24 × 10-7	0.13
cg17589341	SLC14A1	7.35 × 10-10	0.13
cg22679120	SNX8	8.16 × 10-9	0.14
cg20828084	CEMIP	2.00 × 10-8	0.14
cg19305227	SLC28A2	1.11 × 10-6	0.14
cg00168942	GJD4	1.18 × 10-7	0.14
cg13038560	C2orf47	8.96 × 10-8	0.15
cg26614073	SCAP	1.33 × 10-9	0.15
cg19346193	UROS	2.09 × 10-8	0.16
cg00075967	STRA6	4.87 × 10-7	0.16
cg03103192	SPATA18	6.07 × 10-12	0.16
cg10345936	SLC36A2	4.09 × 10-6	0.16
cg17099569	GLI2	9.04 × 10-7	0.16
cg04126866	C10orf99	4.23 × 10-10	0.18
cg00091693	KRT20	1.17 × 10-8	0.19

N: Normal; Ad: Adenoma; CRC: Colorectal cancer.

Table 5 Genes showing inverse DNA methylation and gene expression data

Gene symbol	Gene name	DNA methylation			Gene expression
		cg ID	P value	Δβ	Affymetrix ID	P value	LogFC
CRC vs N
AKT3	AKT serine/threonine kinase 3	cg11314684	4.55 × 10-5	-0.16	224229_s_at	0.015	0.07
					222880_at	2.26 × 10-3	0.61
					212609_s_at	4.72 × 10-4	0.62
					212607_at	9.76 × 10-4	0.64
ERG	v-ets avian erythroblastosis virus E26 oncogene homolog	cg17274064	1.48 × 10-4	-0.11	211626_x_at	5.84 × 10-4	0.20
					241926_s_at	2.05 × 10-5	0.64
					213541_s_at	4.69 × 10-9	1.08
MGP	Matrix Gla protein	cg00431549	0.020	-0.12	202291_s_at	2.34 × 10-11	1.97
PIPOX	Pipecolic acid and sarcosine oxidase	cg06144905	1.81 × 10-7	-0.22	221605_s_at	0.019	0.50
CCR10	C-C motif chemokine receptor 10	cg09509673	1.91 × 10-4	0.11	220565_at	0.010	-0.51
CLEC3B	C-type lectin domain family 3 member B	cg06117855	1.88 × 10-3	0.11	205200_at	1.45 × 10-13	-1.79
LTK	Leukocyte receptor tyrosine kinase	cg27494383	9.32 × 10-3	0.12	217184_s_at	2.14 × 10-11	-1.32
					207106_s_at	2.00 × 10-5	-0.75
PPP1R16B	Protein phosphatase 1 regulatory subunit 16B	cg24834740	3.56 × 10-12	0.37	41577_at	1.76 × 10-4	-0.79
					212750_at	7.68 × 10-3	-0.48
PRKAA2	Protein kinase AMP-activated catalytic subunit alpha 2	cg06836772	0.013	0.14	227892_at	4.15 × 10-3	-0.87
					238489_at	4.67 × 10-3	-0.22
SFRP1	Secreted frizzled related protein 1	cg02388150	3.33 × 10-3	0.10	202036_s_at	8.66 × 10-8	-1.47
					202037_s_at	3.98 × 10-5	-1.11
					202035_s_at	0.039	-0.28
SYNE1	Spectrin repeat containing nuclear envelope protein 1	cg26620959	1.32 × 10-6	0.30	209447_at	4.27 × 10-5	-0.63
AD vs N
CEMIP	Cell migration inducing hyaluronan binding protein	cg20828084	1.33 × 10-9	-0.17	1554685_a_at	1.71 × 10-11	1.12
					212942_s_at	2.41 × 10-43	6.88
NT5DC3	5'-nucleotidase domain containing 3	cg06462291	7.45 × 10-5	-0.25	218786_at	6.83 × 10-9	0.76
SPATA18	Spermatogenesis associated 18	cg03103192	1.39 × 10-7	-0.15	230723_at	4.41 × 10-5	0.45
					229331_at	1.20 × 10-10	1.50
DKK3	Dickkopf WNT signaling pathway inhibitor 3	cg13216057	9.64 × 10-4	0.18	202196_s_at	9.82 × 10-5	-0.65
					214247_s_at	1.52 × 10-4	-0.50
PPP1R16B	Protein phosphatase 1 regulatory subunit 16B	cg24834740	2.23 × 10-9	0.36	41577_at	1.20 × 10-10	-1.44
					212750_at	3.28 × 10-7	-0.81
SDC2	Syndecan 2	cg25070637	1.82 × 10-5	0.30	212158_at	1.74 × 10-10	-1.17
					212157_at	2.14 × 10-8	-0.66
					212154_at	9.19 × 10-5	-0.64
SFRP1	Secreted frizzled related protein 1	cg02388150	1.20 × 10-7	0.15	202036_s_at	3.62 × 10-13	-1.79
					202037_s_at	4.43 × 10-11	-1.48
					202035_s_at	0.016	-0.32
SYNE1	Spectrin repeat containing nuclear envelope protein 1	cg26620959	3.89 × 10-6	0.29	209447_at	5.76 × 10-15	-1.19
CRC vs AD
CEMIP	Cell migration inducing hyaluronan binding protein	cg20828084	2.00 × 10-8	0.14	212942_s_at	9.88 × 10-3	-1.17
SLC28A2	Solute carrier family 28 member 2	cg19305227	1.11 × 10-6	0.14	207249_s_at	0.027	-1.24
SPATA18	Spermatogenesis associated 18	cg03103192	6.07 × 10-12	0.16	229331_at	3.52 × 10-9	-1.73
					230723_at	7.35 × 10-4	-0.41

N: Normal; Ad: Adenoma; CRC: Colorectal cancer.

Table 6 Whole promoter methylation status of genes with inverse age-related CpG site DNA methylation and gene expression data

Gene symbol	DNA methylation on age-related CpG site			Whole promoter DNA methylation status
	cg ID	P value	Δβ	Start-Stop position	P value	Δβ
CRC vs N
AKT3	cg11314684	4.55 × 10-5	-0.16	chr1:244005801-244005900	6.56 × 10-3	-0.33
ERG	cg17274064	1.48 × 10-4	-0.11	chr21:39871501-39871600	0.044	-0.21
MGP	cg00431549	0.020	-0.12	chr12:15038501-15038600	0.030	-0.27
CCR10	cg09509673	1.91 × 10-4	0.11	chr17:40835101-40835200	0.045	0.18
				chr17:40835201-40835300	0.022	0.26
PPP1R16B	cg24834740	3.56 × 10-12	0.37	chr20:37433201-37433300	3.76 × 10-3	0.35
				chr20:37434701-37434800	0.048	0.40
				chr20:37435301-37435400	0.042	0.37
SFRP1	cg02388150	3.33 × 10-3	0.10	chr8:41166001-41166100	0.017	0.44
				chr8:41166101-41166200	1.31 × 10-4	0.56
				chr8:41166201-41166300	3.40 × 10-3	0.49
				chr8:41166301-41166400	0.014	0.27
				chr8:41166401-41166500	1.28 × 10-3	0.53
				chr8:41166501-41166600	0.027	0.44
				chr8:41166601-41166700	0.034	0.39
				chr8:41166901-41167000	0.042	0.41
SYNE1	cg26620959	1.32 × 10-6	0.30	chr6:152622201-152622300	0.027	0.35
				chr6:152957601-152957700	0.032	0.39
				chr6:152957701-152957800	0.043	0.40
				chr6:152958101-152958200	2.86 × 10-4	0.65
AD vs N
CEMIP	cg20828084	1.33 × 10-9	-0.17	chr15: 81070701-81070800	0.028	-0.25
SPATA18	cg03103192	1.39 × 10-7	-0.15	chr4:52915901-52916000	0.042	-0.23
DKK3	cg13216057	9.64 × 10-4	0.18	chr11:12029701-12029800	3.11 × 10-3	0.26
				chr11:12029801-12029900	1.67 × 10-3	0.33
				chr11:12029901-12030000	8.08 × 10-5	0.56
				chr11:12030001-12030100	0.047	0.15
				chr11:12030401-12030500	7.38 × 10-3	0.28
				chr11: 12030501-12030600	0.012	0.28
PPP1R16B	cg24834740	2.23 × 10-9	0.36	chr20:37434201-37434300	0.045	0.26
				chr20:37434601-37434700	0.022	0.39
				chr20:37434701-37434800	0.013	0.39
				chr20:37434801-37434900	6.87 × 10-3	0.30
				chr20:37434901-37435000	1.70 × 10-3	0.44
				chr20:37435001-37435100	1.93 × 10-3	0.45
				chr20:37435101-37435200	6.49 × 10-3	0.35
				chr20:37435201-37435300	8.93 × 10-4	0.51
				chr20:37435301-37435400	0.014	0.42
SDC2	cg25070637	1.82 × 10-5	0.30	chr8:97505901-97506000	2.30 × 10-3	0.59
				chr8:97506101-97506200	1.96 × 10-4	0.65
				chr8:97506201-97506300	8.43 × 10-4	0.63
				chr8:97506301-97506400	0.012	0.49
				chr8:97506401-97506500	0.019	0.47
				chr8:97506601-97506700	3.99 × 10-3	0.51
				chr8:97506701-97506800	0.021	0.45
				chr8:97506801-97506900	0.014	0.52
				chr8:97507101-97507200	0.015	0.44
				chr8:97507201-97507300	0.010	0.52
				chr8:97507301-97507400	3.47 × 10-3	0.59
SFRP1	cg02388150	1.20 × 10-7	0.15	chr8:41165901-41166000	2.30 × 10-3	0.59
				chr8:41166001-41166100	1.57 × 10-3	0.59
				chr8:41166101-41166200	3.54 × 10-4	0.56
				chr8:41166201-41166300	1.49 × 10-4	0.60
				chr8:41166301-41166400	6.54 × 10-3	0.27
				chr8:41166401-41166500	1.32 × 10-5	0.55
				chr8: 41166501-41166600	2.30 × 10-3	0.60
				chr8:41166601-41166700	5.45 × 10-3	0.57
				chr8:41166701-41166800	8.15 × 10-3	0.51
				chr8:41166801-41166900	0.032	0.39
				chr8:41166901-41167000	0.019	0.45
				chr8:41167001-41167100	0.046	0.34
				chr8:41167101-41167200	0.025	0.41
				chr8:41167201-41167300	0.024	0.40
SYNE1	cg26620959	3.89 × 10-6	0.29	chr6:152702701-152702800	0.012	0.20
				chr6:152702801-152702900	0.035	0.21
				chr6:152957501-152957600	6.05 × 10-4	0.37
				chr6:152957601-152957700	2.34 × 10-4	0.60
				chr6:152957701-152957800	0.017	0.51
				chr6:152957801-152957900	1.09 × 10-3	0.42
				chr6:152957901-152958000	1.03 × 10-4	0.57
				chr6:152958001-152958100	7.13 × 10-4	0.43
				chr6:152958101-152958200	1.72 × 10-5	0.72
CRC vs AD
CEMIP	cg20828084	2.00 × 10-8	0.14	chr15:81070701-81070800	7.59 × 10-4	0.31
				chr15:81070801-81070900	0.011	0.36

Only significant promoter DNA methylation alterations are represented in the Table. P < 0.05.