Transient receptor potential vanilloid 1-immunoreactive signals in murine enteric glial cells

Table 1 Summary of the primary antibodies used in this study

Antibody	Provider	Source and type	Product/clone number	Immunogen	Usage
TRPV1	Bios	Rabbit polyclonal	bs-1931R	a.a.825-835 of human TRPV1	Figures 1-3, Suppl. Figure 1
				(EDAEVFKDSMAPGEK)
	LifeSpan	Mouse monoclonal	LS-C122800	a.a. 819-835 of rat TRPV1 (CGSLKPEDAEVFKDSMVPGEK)	Figure 6, Figure 7, Suppl. Figure 1
GFAP	BD	Mouse monoclonal	cocktail of 4A11, 1B4 and 2E1	cow spinal cord homogenate (4A11, 1B4) or human /bovine GFAP (2E1)	Figures 1-3, Suppl. Figure 1
	DAKO	Rabbit polyclonal	Z0344	isolated GFAP from cow spinal cord	Figure 6, Figure 7
	CST	Mouse monoclonal	GA5	isolated GFAP from pig spinal cord	Suppl. Figure 2
PGP9.4	Abcam	Guinea pig serum	ab10410	a.a. 175-191 of human PGP9.5 (GASSEDTLLKDAAKVCL)	Figure 6, Figure 7
S100β	Proteintech	Rabbit polyclonal	15146-1-AP	recombinant human S100β	Figure 7, Figure 8, Suppl. Figure 3
αSMA	Novus	Rabbit monoclonal	E184	synthetic peptides corresponding to N-terminus (human)	Suppl. Figure 2

The negative controls of immunostaining performed in this study are shown in Supplementary Figure S6. Corresponding sequence of mouse TRPV1: TGSLKPEDAEVFKDSMAPGEK. The amino acid underlined is different from rat and the amino acid double-underlined is different from human. TRPV1 KO mice used in the present study preserved this sequence but the signal of immunohistochemistry in KO mice is faint, even in case detected, compared to that in WT mice, in the same staining and signal development protocols on the same slide on which the specimens from KO and WT mice were mounted together. Similar observations have been reported by Yamada et al, J Histochem Cytochem 2009; 57: 277-287.