Copyright
©The Author(s) 2016.
World J Gastroenterol. Oct 14, 2016; 22(38): 8596-8604
Published online Oct 14, 2016. doi: 10.3748/wjg.v22.i38.8596
Published online Oct 14, 2016. doi: 10.3748/wjg.v22.i38.8596
Table 1 Immunohistochemical procedures
Antigen (Clone) | Manufacturer | Antigen retrieval | Dilution | Incubation |
solution/temperature/time | temperature/time | |||
Synaptophysin (27G12) | Nichirei Biosciences, Tokyo, Japan | TB/105 °C/10 min | Prediluted | RT/30 min |
Chromogranin A (polyclonal) | Dako Japan, Tokyo, Japan | CB/115 °C/10 min | 1:500 | RT/30 min |
Pan-cytokeratin (AE1/AE3) | Dako Japan, Tokyo, Japan | TB/105 °C/10 min | 1:100 | RT/30 min |
E-cadherin (36B5) | Thermo Scientific, Yokohama, Japan | TB/121 °C/10 min | 1:20 | RT/60 min |
Progesterone receptor (IE2) | Roche Diagnostics, Tokyo, Japan | CB/115 °C/10 min | Prediluted | RT/30 min |
Vimentin (V9) | Dako Japan, Tokyo, Japan | CB/100 °C/15 min | 1:100 | RT/30 min |
α-1-antitrypsin (polyclonal) | Dako Japan, Tokyo, Japan | No retrieval | 1:100 | RT/30 min |
CD10 (56C6) | Leica Microsystems, Tokyo, Japan | TB/105 °C/10 min | 1:40 | RT/30 min |
β-catenin (β-Catenin-1) | Dako Japan, Tokyo, Japan | TB/105 °C/10 min | 1:200 | RT/30 min |
Ki-67 (MIB-1) | Dako Japan, Tokyo, Japan | CB/100 °C/30 min | 1:25 | 4 °C/overnight |
Table 2 Clinicopathological and morphological characteristics of neuroendocrine tumor (24 cases) and solid-pseudopapillary neoplasm (6 cases)
Case | Age | Sex | Clinical findings | Tumor location | Operation | Diameter (cm) | Mitotic | Ki-67 | Grade | Solid or | PP |
(yr) | count | (%) | for NETs | Cystic | pattern | ||||||
NET-1 | 31 | F | MEN1 | H | PD | 4.5 | 0 | 0 | 1 | Solid and Cystic | Absent |
NET-2 | 57 | F | MEN1 | H, T | EC, DP | 5.3 | 0 | 1 | 1 | Solid | Absent |
NET-3 | 51 | F | MEN1 | H, B, T, L | EC, DP, LR | 6.0 | 0 | 0 | 1 | Solid | Absent |
NET-4 | 45 | F | MEN1 | B, T | DP | 1.4 | 0 | 0 | 1 | Solid and Cystic | Absent |
NET-5 | 42 | F | insulinoma | B | EC | 1.0 | 0 | 0 | 1 | Solid | Absent |
NET-6 | 62 | F | insulinoma | H | EC | 1.2 | 0 | 0 | 1 | Solid | Absent |
NET-7 | 73 | M | insulinoma | H | PD | 1.5 | 0 | 0 | 1 | Solid | Absent |
NET-8 | 74 | F | insulinoma | T | DP | 1.0 | 0 | 0 | 1 | Solid | Absent |
NET-9 | 67 | M | insulinoma | B | EC | 0.9 | 1 | 0 | 1 | Solid | Absent |
NET-10 | 51 | F | insulinoma | B | MP | 1.1 | 7 | 2 | 2 | Solid | Absent |
NET-11 | 45 | M | H | EC | 2.3 | 0 | 0 | 1 | Solid | Absent | |
NET-12 | 68 | F | H | EC | 0.5 | 0 | 0 | 1 | Solid | Absent | |
NET-13 | 77 | M | T | DP | 0.9 | 1 | 0 | 1 | Solid | Absent | |
NET-14 | 57 | M | H | PD | 1.5 | 1 | 0 | 1 | Solid | Absent | |
NET-15 | 45 | M | H | EC | 0.9 | 2 | 0 | 1 | Solid | Absent | |
NET-16 | 51 | F | H | PD | 2.5 | 0 | 1 | 1 | Solid | Absent | |
NET-17 | 59 | M | H | MP | 1.8 | 1 | 1 | 1 | Solid | Absent | |
NET-18 | 59 | F | T | EC | 2.3 | 1 | 1 | 1 | Solid and Cystic | Absent | |
NET-19 | 40 | F | T | DP | 3.3 | 0 | 4 | 2 | Solid and Cystic | Absent | |
NET-20 | 53 | M | T | DP | 5.5 | 2 | 3 | 2 | Solid | Absent | |
NET-21 | 39 | M | H, B, T | TP | 16 | 3 | 0 | 2 | Solid | Absent | |
NET-22 | 51 | M | H | PD | 7.0 | 7 | 4 | 2 | Solid | Absent | |
NET-23 | 58 | F | B | EC | 1.8 | 1 | 0 | 1 | Solid | Absent | |
NET-24 | 32 | M | H | PD | 2.3 | 0 | 0 | 1 | Solid | Absent | |
SPN-1 | 26 | F | H | EC | 8.5 | 0 | 0 | - | Solid and Cystic | Present | |
SPN-2 | 34 | F | T | DP | 7.0 | 1 | 1 | - | Solid and Cystic | Present | |
SPN-3 | 20 | F | T | DP | 7.5 | 0 | 0 | - | Solid and Cystic | Present | |
SPN-4 | 23 | F | T | DP | 13 | 0 | 0 | - | Solid and Cystic | Present | |
SPN-5 | 43 | F | B | MP | 3.8 | 0 | 0 | - | Solid and Cystic | Present | |
SPN-6 | 27 | M | T | DP | 4.3 | 0 | 0 | - | Solid and Cystic | Present |
Table 3 Immunohistochemical profiles of neuroendocrine tumor and solid-pseudopapillary neoplasm
Case | Syn | CgA | CK | E-cad | PgR | Vim | αATP | CD10 | βcat (N/C) |
NET-1 | ++ | ++ | ++ | ++ | - | - | + | ++ | - |
NET-2 | ++ | ++ | ++ | ++ | ++ | - | + | ++ | - |
NET-3 | ++ | ++ | ++ | ++ | ++ | - | ++ | ++ | - |
NET-4 | ++ | ++ | ++ | ++ | ++ | ++ | - | - | - |
NET-5 | ++ | ++ | + | ++ | ++ | - | - | - | - |
NET-6 | ++ | ++ | + | ++ | ++ | - | - | - | - |
NET-7 | ++ | ++ | ++ | ++ | ++ | - | ++ | - | - |
NET-8 | ++ | ++ | + | ++ | ++ | - | ++ | - | - |
NET-9 | ++ | ++ | - | ++ | - | - | - | - | - |
NET-10 | ++ | ++ | + | ++ | ++ | ++ | ++ | - | - |
NET-11 | ++ | ++ | - | ++ | ++ | - | + | - | - |
NET-12 | ++ | ++ | + | + | ++ | ++ | - | - | - |
NET-13 | ++ | ++ | ++ | ++ | ++ | ++ | - | - | - |
NET-14 | ++ | ++ | ++ | ++ | - | ++ | ++ | - | - |
NET-15 | ++ | ++ | ++ | ++ | ++ | - | - | - | - |
NET-16 | ++ | ++ | + | + | ++ | - | ++ | ++ | - |
NET-17 | ++ | ++ | ++ | ++ | ++ | - | - | ++ | - |
NET-18 | ++ | ++ | ++ | ++ | ++ | ++ | - | ++ | - |
NET-19 | ++ | ++ | ++ | ++ | ++ | ++ | - | - | - |
NET-20 | ++ | ++ | ++ | ++ | ++ | ++ | ++ | - | - |
NET-21 | ++ | ++ | ++ | ++ | ++ | - | ++ | - | - |
NET-22 | ++ | ++ | ++ | ++ | - | - | ++ | - | - |
NET-23 | ++ | + (dot) | - | - | ++ | ++ | ++ | ++ | ++ |
NET-24 | ++ | + (dot) | - | - | ++ | ++ | ++ | ++ | ++ |
SPN-1 | - | + (dot) | - | - | ++ | ++ | ++ | ++ | ++ |
SPN-2 | - | + (dot) | - | - | ++ | ++ | ++ | ++ | ++ |
SPN-3 | ++ | + (dot) | + | - | ++ | ++ | ++ | ++ | ++ |
SPN-4 | ++ | + (dot) | - | - | ++ | ++ | ++ | ++ | ++ |
SPN-5 | ++ | + (dot) | - | - | ++ | ++ | ++ | ++ | ++ |
SPN-6 | ++ | + (dot) | - | - | ++ | ++ | ++ | ++ | ++ |
- Citation: Ohara Y, Oda T, Hashimoto S, Akashi Y, Miyamoto R, Enomoto T, Satomi K, Morishita Y, Ohkohchi N. Pancreatic neuroendocrine tumor and solid-pseudopapillary neoplasm: Key immunohistochemical profiles for differential diagnosis. World J Gastroenterol 2016; 22(38): 8596-8604
- URL: https://www.wjgnet.com/1007-9327/full/v22/i38/8596.htm
- DOI: https://dx.doi.org/10.3748/wjg.v22.i38.8596