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©The Author(s) 2016.
World J Gastroenterol. Oct 14, 2016; 22(38): 8528-8539
Published online Oct 14, 2016. doi: 10.3748/wjg.v22.i38.8528
Published online Oct 14, 2016. doi: 10.3748/wjg.v22.i38.8528
Table 1 Primer sequences used in quantitative real-time polymerase chain reaction for detection of stem cell marker mRNA expression
Gene | Sequence |
CD133-F | 5’ TTTGTCTTCTATTCTTGGCTTC 3’ |
CD133-R | 5’ ACCTTGTCATAATCAATTTTGG 3’ |
CD44-F | 5’ GGTTCATAGAAGGGCACGT 3’ |
CD44-R | 5’ TGTCTTCGTCTGGGATGG 3’ |
CD24-F | 5’ TCAAGTATTTGGGAAGTG 3’ |
CD24-R | 5’ GTGTTCTAAATGTGGCTAT 3’ |
OCT3/4-F | 5’ CGACCATCTGCCGCTTTGAG 3’ |
OCT3/4-R | 5’ CCCCCTGTCCCCCATTCCTA 3’ |
KLF4-F | 5’ TGGGTCTTGAGGAAGTGCTG 3’ |
KLF4-R | 5’ TGTTTACGGTAGTGCCTGGTC 3’ |
SOX2-F | 5’ CACCTACAGCATGTCCTACTC 3’ |
SOX2-R | 5’ CATGCTGTTTCTTACTCTCCTC 3’ |
PRL-1-F | 5’ GGCAAACTTCGAGTCTCCT 3’ |
PRL-1-R | 5’ CCGGTTGATGAATGGCTAA 3’ |
MEIS2-F | 5’ GTCCACGAACTGTGCGATAA 3’ |
MEIS2-R | 5’ TTCGGAAGGGTACGGATG 3’ |
AXIN2-F | 5’ AGCATTTTAATCAACAGCATCTA 3’ |
AXIN2-R | 5’ TAACTAAGAATGTGATCCAAGAA 3’ |
NANOG-F | 5’ CAACTGGCCGAAGAATAGCA 3’ |
NANOG-R | 5’ GCAGGAGAATTTGGCTGGAA 3’ |
GAPDH-F | 5’ GGAGCGAGATCCCTCCAAAAT 3’ |
GAPDH-R | 5’ GGCTGTTGTCATACTTCTCATGG 3’ |
Table 2 Primer sequences used in polymerase chain reaction for detecting genomic binding sites for stem cell markers
Gene | Sequence |
CD133-F | 5’ TTTGTCTTCTATTCTTGGCTTC 3’ |
CD133-R | 5’ ACCTTGTCATAATCAATTTTGG 3’ |
CD44(1)-F | 5’ CTCATGGCTCAGTCGCCCAATCA 3’ |
CD44(1)-R | 5’ TTTGCTCCTGAGCTGTTGCGTGG 3’ |
CD44(2)-F | 5’ AGATTAAGGAGCTAGGACTC 3’ |
CD44(2)-R | 5’ AAGATCACTTGGCAAGAAAG 3’ |
CD44(3)-F | 5’ GGCACGTGTGAAACCTTTCCATTC 3’ |
CD44(3)-R | 5’ GCTGAGCTGGACGCCAAGCA 3’ |
CD44(4)-F | 5’ GCCTTTCATCCCTCGGGTGTGC 3’ |
CD44(4)-R | 5’ TTCCTCCCAGGGACCAGGCC 3’ |
MEIS2(1)-F | 5’ GGATTCCTGGCCAAAGGACGC 3’ |
MEIS2(1)-R | 5’ CTCCCCCTAAGAGCGGCTCCA 3’ |
MEIS2(2)-F | 5’ ACTGCCCGCAAGGATTCCACAA 3’ |
MEIS2(2)-R | 5’ GGACTGTGGACCAAATCCAGCACAG 3’ |
AXIN2-F | 5’ TATTCAAGGCATCTTTTACTGGAC 3’ |
AXIN2-R | 5’ AGCAAAGAACTAGCCAATAAGGAG 3’ |
- Citation: Li Y, Liu YH, Hu YY, Chen L, Li JM. Special AT-rich sequence-binding protein 2 acts as a negative regulator of stemness in colorectal cancer cells. World J Gastroenterol 2016; 22(38): 8528-8539
- URL: https://www.wjgnet.com/1007-9327/full/v22/i38/8528.htm
- DOI: https://dx.doi.org/10.3748/wjg.v22.i38.8528