Special AT-rich sequence-binding protein 2 acts as a negative regulator of stemness in colorectal cancer cells

Table 1 Primer sequences used in quantitative real-time polymerase chain reaction for detection of stem cell marker mRNA expression

Gene	Sequence
CD133-F	5’ TTTGTCTTCTATTCTTGGCTTC 3’
CD133-R	5’ ACCTTGTCATAATCAATTTTGG 3’
CD44-F	5’ GGTTCATAGAAGGGCACGT 3’
CD44-R	5’ TGTCTTCGTCTGGGATGG 3’
CD24-F	5’ TCAAGTATTTGGGAAGTG 3’
CD24-R	5’ GTGTTCTAAATGTGGCTAT 3’
OCT3/4-F	5’ CGACCATCTGCCGCTTTGAG 3’
OCT3/4-R	5’ CCCCCTGTCCCCCATTCCTA 3’
KLF4-F	5’ TGGGTCTTGAGGAAGTGCTG 3’
KLF4-R	5’ TGTTTACGGTAGTGCCTGGTC 3’
SOX2-F	5’ CACCTACAGCATGTCCTACTC 3’
SOX2-R	5’ CATGCTGTTTCTTACTCTCCTC 3’
PRL-1-F	5’ GGCAAACTTCGAGTCTCCT 3’
PRL-1-R	5’ CCGGTTGATGAATGGCTAA 3’
MEIS2-F	5’ GTCCACGAACTGTGCGATAA 3’
MEIS2-R	5’ TTCGGAAGGGTACGGATG 3’
AXIN2-F	5’ AGCATTTTAATCAACAGCATCTA 3’
AXIN2-R	5’ TAACTAAGAATGTGATCCAAGAA 3’
NANOG-F	5’ CAACTGGCCGAAGAATAGCA 3’
NANOG-R	5’ GCAGGAGAATTTGGCTGGAA 3’
GAPDH-F	5’ GGAGCGAGATCCCTCCAAAAT 3’
GAPDH-R	5’ GGCTGTTGTCATACTTCTCATGG 3’

Table 2 Primer sequences used in polymerase chain reaction for detecting genomic binding sites for stem cell markers

Gene	Sequence
CD133-F	5’ TTTGTCTTCTATTCTTGGCTTC 3’
CD133-R	5’ ACCTTGTCATAATCAATTTTGG 3’
CD44(1)-F	5’ CTCATGGCTCAGTCGCCCAATCA 3’
CD44(1)-R	5’ TTTGCTCCTGAGCTGTTGCGTGG 3’
CD44(2)-F	5’ AGATTAAGGAGCTAGGACTC 3’
CD44(2)-R	5’ AAGATCACTTGGCAAGAAAG 3’
CD44(3)-F	5’ GGCACGTGTGAAACCTTTCCATTC 3’
CD44(3)-R	5’ GCTGAGCTGGACGCCAAGCA 3’
CD44(4)-F	5’ GCCTTTCATCCCTCGGGTGTGC 3’
CD44(4)-R	5’ TTCCTCCCAGGGACCAGGCC 3’
MEIS2(1)-F	5’ GGATTCCTGGCCAAAGGACGC 3’
MEIS2(1)-R	5’ CTCCCCCTAAGAGCGGCTCCA 3’
MEIS2(2)-F	5’ ACTGCCCGCAAGGATTCCACAA 3’
MEIS2(2)-R	5’ GGACTGTGGACCAAATCCAGCACAG 3’
AXIN2-F	5’ TATTCAAGGCATCTTTTACTGGAC 3’
AXIN2-R	5’ AGCAAAGAACTAGCCAATAAGGAG 3’