Characterisation of colonic dysplasia-like epithelial atypia in murine colitis

Table 1 Incidence and severity of dysplasia-like atypia

Group	Non-dysplastic	Low	High	Submucosal
C57BL/6J	4/4 (100%)	0/4 (0%)	0/4 (0%)	0/4 (0%)
C57BL/6J + DSS	5/6 (83%)	1/6 (17%)	0/6 (0%)	0/6 (0%)
Winnie	5/6 (83%)	1/6 (17%)	0/6 (0%)	1/6 (17%)
Winnie + DSS	0/11 (0%)	5/11 (45%)	6/11 (55%)	3/11 (27%)

Incidence of graded histological lesions in the distal colon of wild-type C57BL/6 and Winnie mice administered three cycles of DSS. Crypt lesions were graded as either non-dysplastic, low-grade or high-grade dysplasia and dysplasia with submucosal extension. The maximum grade observed was recorded for each animal. The incidence of dysplasia-like lesions in Winnie mice was increased by administration of DSS (Mantel-Haenszel χ² = 6.667, P < 0.01).

Table 2 Spearman’s correlation between mRNA transcript abundance and dysplasia severity

Gene	Restimate	P value
Cav1	-0.19	0.34
Ccl5	0.46	0.017a
Cdkn2a	0.11	0.61
Cxcl5	0.29	0.15a
Cxcl12	0.022	0.92
Il6	0.27	0.20
Muc1	-0.13	0.52
Myc	-0.26	0.20
Pparg	0.18	0.39
Prom1	0.18	0.39
Ptgs2	0.18	0.42
Spp1	0.40	0.049
Trp53	0.15	0.47
Vim	-0.14	0.51

Relationship between relative mRNA abundance (ΔΔCT) and severity of dysplasia in the distal colon was tested using Spearman’s rank correlation. Estimate of Spearman’s co-efficient of correlation (ρ) and corresponding P value for each gene are given,

^aP < 0.05.