Carbonic anhydrase enzymes II, VII, IX and XII in colorectal carcinomas

Table 1 Patient characteristics

	n (%)
Total	539 (100)
Sex
Male	294 (54.5)
Female	245 (45.5)
Age
< 65 yr	231 (42.9)
≥ 65 yr	308 (57.1)
Tumor location
Right side of colon	157 (29.1)
Left side of colon	138 (25.6)
Rectum	244 (45.3)
Stage
Dukes A	82 (15.2)
Dukes B	191 (35.4)
Dukes C	136 (25.2)
Dukes D	130 (24.1)
Differentiation (grade)
High	19 (3.5)
Medium high	352 (65.3)
Medium low	141 (26.2)
Low	27 (5.0)

Table 2 Summary of carbonic anhydrases stainings

	Mean	SD	Median	Mode	25%	75%
CA II epithelium intensity	1.8	1.1	2	3	1	3
CA II epithelium extent	2.2	1.1	3	3	2	3
CA II endothelium	0.9	0.3	1	1	1	1
CA VII epithelium intensity	1.4	1	1	1	1	2
CA VII epithelium extent	2	1.2	3	3	1	3
CA VII stromal intensity	2.8	0.5	3	3	3	3
CA VII stromal extent	2.8	0.5	3	3	3	3
CA IX epithelium intensity	1.7	1.2	2	3	0	3
CA IX epithelium extent	1.6	1.2	2	3	0	3
CA XII epithelium intensity	1.5	0.9	1	2	1	2
CA XII epithelium extent	2	1.1	2	3	1	3

Table 3 Multivariate Cox regression analysis results of selected parameters

	Hazard ratio	95%CIlower	95%CIupper	P value
CA II epithelium intensity analysis
CA II epithelium intensity	1.19	1.04	1.37	0.0092
Sex (men vs females)	1.20	0.92	1.58	0.1777
Age over 65 yr	1.78	1.35	2.35	P < 0.0001
Side of colon (sin vs dex)	1.17	0.86	1.58	0.3170
CA II epithelium extent analysis, age ≤ 65 yr
CA II epithelium extent	0.88	0.73	1.06	0.1742
Sex (men vs females)	0.99	0.65	1.51	0.9564
Side of colon (sin vs dex)	1.63	0.97	2.74	0.1869
CA II epithelium extent analysis, age > 65 yr
CA II epithelium extent	1.42	1.16	1.73	0.0006
Sex (men vs females)	1.44	1.01	2.05	0.0435
Side of colon (sin vs dex)	0.92	0.63	1.35	0.6764
CA XII epithelium intensity analysis
CA XII epithelium intensity	1.18	1.01	1.38	0.0360
Sex (men vs females)	1.23	0.94	1.60	0.1301
Age over 65 yr	1.67	1.27	2.19	0.0002
Side of colon (sin vs dex)	1.09	0.81	1.47	0.5571

Regression analyses are stratified by Dukes and differentiation status. Separate analyses were done for each carbonic anhydrases (CAs) staining. Due to interaction between age and epithelium extent, the analysis was split in different age groups.

Table 4 Carbonic anhydrases VII and Dukes stage

CA VII epithelial extent	Dukes A count	%	B count	%	C count	%	D count	%
0	15	16.1%	36	38.7%	25	26.9%	17	18.3%
1	14	19.7%	31	43.7%	14	19.7%	12	16.9%
2	10	15.2%	23	34.8%	14	21.2%	19	28.8%
3	28	11.4%	85	34.6%	67	27.2%	66	26.8%

P = 0.013, linear by linear association test.