Synergistic anticancer effect of exogenous wild-type p53 gene combined with 5-FU in human colon cancer resistant to 5-FU in vivo

doi:10.3748/wjg.v22.i32.7342

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Aug 28, 2016; 22(32): 7342-7352
Published online Aug 28, 2016. doi: 10.3748/wjg.v22.i32.7342

Table 1 Tumor apoptosis ratios in experimental groups and control group (mean ± SD)

Group	24 h	48 h	72 h	120 h	168 h
Control	0.25 ± 0.02	0.28 ± 0.01	0.27 ± 0.02	0.29 ± 0.02	0.24 ± 0.04
5-FU	0.27 ± 0.03	0.31 ± 0.03	0.31 ± 0.05	0.30 ± 0.04	0.31 ± 0.02
rAd-p53	0.29 ± 0.02	0.35 ± 0.03	0.43 ± 0.08	0.42 ± 0.06	0.46 ± 0.06
5-FU + rAd-p53	0.33 ± 0.03	0.44 ± 0.08	0.58 ± 0.07	0.59 ± 0.05	0.62 ± 0.07
F	13.235	41.487	53.812	61.676	80.755
P value	0	0	0	0	0
P value	> 0.05	> 0.05	> 0.05	> 0.05	> 0.05
5-FU vs Control	> 0.05	> 0.05	> 0.05	> 0.05	> 0.05
P value	< 0.05	< 0.05	< 0.05	< 0.05	< 0.05
rAd-p53 vs Control	< 0.05	< 0.05	< 0.05	< 0.05	< 0.05
P value	< 0.05	< 0.05	< 0.05	< 0.05	< 0.05
rAd-p53 + 5-FU vs Control	< 0.05	< 0.05	< 0.05	< 0.05	< 0.05
P value	< 0.05	< 0.05	< 0.05	< 0.05	< 0.05
rAd-p53 + 5-FU vs rAd-p53	< 0.05	< 0.05	< 0.05	< 0.05	< 0.05
P value	< 0.05	< 0.05	< 0.05	< 0.05	< 0.05
rAd-p53 + 5-FU vs 5-FU	< 0.05	< 0.05	< 0.05	< 0.05	< 0.05
P value	>0.05	< 0.05	< 0.05	< 0.05	< 0.05
rAd-p53 vs 5-FU	>0.05	< 0.05	< 0.05	< 0.05	< 0.05

Table 2 Relative IOD of p53 expression in experimental groups and control group (mean ± SD)

Group	24 h	48 h	72 h	120 h	168 h
Control	0.099 ± 0.001	0.105 ± 0.003	0.101 ± 0.007	0.105 ± 0.015	0.103 ± 0.001
5-FU	0.108 ± 0.002	0.098 ± 0.028	0.096 ± 0.004	0.097 ± 0.047	0.096 ± 0.033
rAd-p53	0.187 ± 0.003	0.209 ± 0.014	0.627 ± 0.005	0.743 ± 0.012	0.713 ± 0.005
5-FU + rAd-p53	0.393 ± 0.004	0.461 ± 0.002	0.691 ± 0.018	0.822 ± 0.009	0.808 ± 0.027
F	221.565	270.392	3983.173	9639.595	3867.703
P value	0	0	0	0	0
P value	> 0.05	> 0.05	> 0.05	> 0.05	> 0.05
5-FU vs Control	> 0.05	> 0.05	> 0.05	> 0.05	> 0.05
P value	< 0.05	< 0.05	< 0.05	< 0.05	< 0.05
rAd-p53 vs Control	< 0.05	< 0.05	< 0.05	< 0.05	< 0.05
P value	< 0.05	< 0.05	< 0.05	< 0.05	< 0.05
rAd-p53 + 5-FU vs Control	< 0.05	< 0.05	< 0.05	< 0.05	< 0.05
P value	< 0.05	< 0.05	< 0.05	< 0.05	< 0.05
rAd-p53 + 5-FU vs rAd-p53	< 0.05	< 0.05	< 0.05	< 0.05	< 0.05
P value	< 0.05	< 0.05	< 0.05	< 0.05	< 0.05
rAd-p53 + 5-FU vs 5-FU	< 0.05	< 0.05	< 0.05	< 0.05	< 0.05
P value	< 0.05	< 0.05	< 0.05	< 0.05	< 0.05
rAd-p53 vs 5-FU	< 0.05	< 0.05	< 0.05	< 0.05	< 0.05

Table 3 Relative IOD of protein kinase C expression in experimental groups and control group (mean ± SD)

Group	24 h	48 h	72 h	120 h	168 h
Control	1.13 ± 0.08	1.21 ± 0.06	0.99 ± 0.13	1.26 ± 0.42	1.08 ± 0.20
5-FU	0.94 ± 0.21	1.12 ± 0.14	0.93 ± 0.14	1.08 ± 0.52	1.04 ± 0.26
rAd-p53	0.93 ± 0.42	0.91 ± 0.23	1.07 ± 0.13	0.57 ± 0.18	0.36 ± 0.09
5-FU + rAd-p53	1.23 ± 0.24	0.53 ± 0.09	0.45 ± 0.10	0.26 ± 0.06	0.08 ± 0.01
F	1.036	33.972	14.348	24.072	71.559
P value	0.427	0	0.01	0	0
P value	> 0.05	> 0.05	> 0.05	> 0.05	> 0.05
5-FU vs Control	> 0.05	> 0.05	> 0.05	> 0.05	> 0.05
P value	> 0.05	< 0.05	> 0.05	< 0.05	< 0.05
rAd-p53 vs Control	> 0.05	< 0.05	> 0.05	< 0.05	< 0.05
P value	> 0.05	< 0.05	< 0.05	< 0.05	< 0.05
rAd/p53 + 5- FU vs Control	> 0.05	< 0.05	< 0.05	< 0.05	< 0.05
P value	> 0.05	< 0.05	< 0.05	< 0.05	< 0.05
rAd-p53 + 5-FU vs rAd-p53	> 0.05	< 0.05	< 0.05	< 0.05	< 0.05
P value	> 0.05	< 0.05	< 0.05	< 0.05	< 0.05
rAd-p53 + 5-FU vs 5-FU	> 0.05	< 0.05	< 0.05	< 0.05	< 0.05
P value	> 0.05	< 0.05	> 0.05	< 0.05	< 0.05
rAd-p53 vs 5-FU	> 0.05	< 0.05	> 0.05	< 0.05	< 0.05

Table 4 Relative IOD of P-gp expression in experimental groups and control group (mean ± SD)

Group	24 h	48 h	72 h	120 h	168 h
Control	1.28 ± 0.20	1.32 ± 0.31	1.36 ± 0.40	1.29 ± 0.43	1.30 ± 0.56
5-FU	1.17 ± 0.37	1.13 ± 0.41	1.17 ± 0.39	1.27 ± 0.47	1.29 ± 0.56
rAd-p53	1.12 ± 0.31	0.69 ± 0.11	0.62 ± 0.02	0.36 ± 0.15	0.26 ± 0.06
5-FU + rAd-p53	0.86 ± 0.17	0.65 ± 0.21	0.59 ± 0.25	0.31 ± 0.01	0.10 ± 0.03
F	5.670	7.301	14.645	9.844	7.971
P value	0.022	0.011	0.001	0.005	0.009
P value	> 0.05	> 0.05	> 0.05	> 0.05	> 0.05
5-FU vs Control	> 0.05	> 0.05	> 0.05	> 0.05	> 0.05
P value	> 0.05	< 0.05	< 0.05	< 0.05	< 0.05
rAd-p53 vs Control	> 0.05	< 0.05	< 0.05	< 0.05	< 0.05
P value	< 0.05	< 0.05	< 0.05	< 0.05	< 0.05
rAd-p53 + 5-FU vs Control	< 0.05	< 0.05	< 0.05	< 0.05	< 0.05
P value	< 0.05	> 0.05	> 0.05	> 0.05	> 0.05
rAd-p53 + 5-FU vs rAd-p53	< 0.05	> 0.05	> 0.05	> 0.05	> 0.05
P value	< 0.05	< 0.05	< 0.05	< 0.05	< 0.05
rAd-p53 + 5-FU vs 5-FU	< 0.05	< 0.05	< 0.05	< 0.05	< 0.05
P value	> 0.05	< 0.05	< 0.05	< 0.05	< 0.05
rAd-p53 vs 5-FU	> 0.05	< 0.05	< 0.05	< 0.05	< 0.05

Table 5 Relative IOD of MRP1 expression in experimental groups and control group (mean ± SD)

Group	24 h	48 h	72 h	120 h	168 h
Control	1.31 ± 0.36	1.33 ± 0.08	1.09 ± 0.24	1.22 ± 0.35	1.07 ± 0.22
5-FU	1.27 ± 0.10	1.31 ± 0.11	1.14 ± 0.16	1.20 ± 0.31	1.13 ± 0.14
rAd-p53	0.98 ± 0.09	0.74 ± 0.11	0.58 ± 0.18	0.87 ± 0.31	0.31 ± 0.08
5-FU + rAd-p53	0.71 ± 0.17	0.62 ± 0.02	0.51 ± 0.10	0.42 ± 0.08	0.13 ± 0.10
F	6.438	123.754	20.567	15.512	36.073
P value	0.016	0	0	0.001	0
P value	> 0.05	> 0.05	> 0.05	> 0.05	> 0.05
5-FU vs Control	> 0.05	> 0.05	> 0.05	> 0.05	> 0.05
P value	> 0.05	< 0.05	< 0.05	< 0.05	< 0.05
rAd-p53 vs Control	> 0.05	< 0.05	< 0.05	< 0.05	< 0.05
P value	< 0.05	< 0.05	< 0.05	< 0.05	< 0.05
rAd-p53 + 5-FU vs Control	< 0.05	< 0.05	< 0.05	< 0.05	< 0.05
P value	> 0.05	< 0.05	> 0.05	< 0.05	> 0.05
rAd-p53 + 5-FU vs rAd-p53	> 0.05	< 0.05	> 0.05	< 0.05	> 0.05
P value	< 0.05	< 0.05	< 0.05	< 0.05	< 0.05
rAd-p53 + 5-FU vs 5-FU	< 0.05	< 0.05	< 0.05	< 0.05	< 0.05
P value	> 0.05	< 0.05	< 0.05	< 0.05	< 0.05
rAd-p53 vs 5-FU	> 0.05	< 0.05	< 0.05	< 0.05	< 0.05

Citation: Xie Q, Wu MY, Zhang DX, Yang YM, Wang BS, Zhang J, Xu J, Zhong WD, Hu JN. Synergistic anticancer effect of exogenous wild-type p53 gene combined with 5-FU in human colon cancer resistant to 5-FU in vivo. World J Gastroenterol 2016; 22(32): 7342-7352
URL: https://www.wjgnet.com/1007-9327/full/v22/i32/7342.htm
DOI: https://dx.doi.org/10.3748/wjg.v22.i32.7342