Visceral hypersensitive rats share common dysbiosis features with irritable bowel syndrome patients

doi:10.3748/wjg.v22.i22.5211

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World Journal of Gastroenterology

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World J Gastroenterol. Jun 14, 2016; 22(22): 5211-5227
Published online Jun 14, 2016. doi: 10.3748/wjg.v22.i22.5211

Table 1 Importance and meaning of the first 10 primary components of fecal microbiota

Primary component	% of variance	Difference among time points (n = 80, df = 2)		Difference among groups (n = 80, df = 3)
Primary component	% of variance	χ²	P-value	χ²	P-value
1	27.7%	37.67931	0.00000¹	4.6999109	0.19514
2	14.6%	1.352165	0.50861	20.4278998	0.00014¹
3	11.4%	6.165561	0.04583¹	8.6830899	0.03382¹
4	8.5%	0.719395	0.69789	21.7793862	0.00007¹
5	6.1%	0.623716	0.73209	4.6400976	0.20013
6	4.5%	8.388254	0.01508¹	9.3688439	0.02477¹
7	4.1%	0.844768	0.65548	0.7871023	0.85255
8	3.2%	0.412918	0.81346	6.6021743	0.08572
9	2.2%	8.880049	0.01180¹	9.9866961	0.01868¹
10	1.7%	1.762893	0.41418	2.6274074	0.45270

¹The P-value is less than 0.05.

Table 2 Weighted and unweighted UniFrac test on experiment groups and co-housing

Time point	Comparison	Number of samples to build phylogenetic tree	Weighted UniFrac test		Unweighted UniFrac test
Time point	Comparison	Number of samples to build phylogenetic tree	Weighted UniFrac score	P-value	Unweighted UniFrac score	P-value
Week 3	Ctl-MS	29	0.863902	< 0.0010	0.957176	< 0.0010¹
	Ctl-MS_D	29	0.926521	< 0.0010	0.963861	0.0130¹
	MS-MS_D	29	0.833168	< 0.0010	0.909242	0.2790
	Ctl(B8)-Ctl(C6)	29	1.000000	< 0.0010	1.000000	< 0.0010¹
	MS(D3)-MS(G5)	29	1.000000	0.0070	1.000000	0.5070
Week 8	Ctl-MS	27	0.719683	< 0.0010	0.951668	0.0190¹
	Ctl(B8)-Ctl(C6)	27	0.83063	< 0.0010	0.955082	0.0870
	MS(D3)-MS(G5)	27	0.617715	< 0.0010	0.874196	0.5220
Week 12	Ctl-MS	24	0.754046	< 0.0010	0.944729	0.0390¹
	Ctl-pTNBS	24	0.942882	< 0.0010	0.978698	0.0160¹
	MS-pTNBS	24	0.828407	< 0.0010	0.973938	0.0130¹
	Ctl(B4)-Ctl(C3)	24	0.928051	< 0.0010	0.976209	0.3200
	MS(D3)-MS(G5)	24	0.771421	< 0.0010	0.912219	0.4550
	pTNBS(B4)-pTNBS(C2)	24	1.000000	< 0.0010	1.000000	0.6390

¹The P-value is less than 0.05. MS: Maternal separation; pTNBS: TNBS post-inflammatory.

Table 3 Abundance of different taxa among groups by week 12 and correlations to visceral hypersensitivity

Taxonomy	LDA score (Log10)	Group	Across groups (n = 24)		Within group
Taxonomy	LDA score (Log10)	Group	r_all_sample	p_all_sample	r_ingroup	p_ingroup
Actinobacteria	2.338	Controln = 7	0.1835	0.3906	0.2041	0.6606
Actinobacteria\|Actinobacteria	2.338		0.1835	0.3906	0.2041	0.6606
Actinobacteria\|Actinobacteria\|Coriobacteriales\|Coriobacteriaceae\|Unclassified	2.192		0.2639	0.2127	0.2041	0.6606
Bacteroidetes\|Bacteroidia\|Bacteroidales\|Porphyromonadaceae	4.610		-0.1005	0.6405	0.1429	0.7825
Bacteroidetes\|Bacteroidia\|Bacteroidales\|Porphyromonadaceae\|Unclassified	4.543		-0.1344	0.5313	0.1429	0.7825
Fibrobacteres	2.435		-0.3601	0.0839	-0.0741	0.8745
Fibrobacteres\|Fibrobacteria	2.435		-0.3601	0.0839	-0.0741	0.8745
Fibrobacteres\|Fibrobacteria\|Fibrobacterales	2.435		-0.3601	0.0839	-0.0741	0.8745
Fibrobacteres\|Fibrobacteria\|Fibrobacterales\|Fibrobacteraceae	2.435		-0.3601	0.0839	-0.0741	0.8745
Fibrobacteres\|Fibrobacteria\|Fibrobacterales\|Fibrobacteraceae\|Fibrobacter	2.435		-0.3601	0.0839	-0.0741	0.8745
Firmicutes\|Bacilli\|Lactobacillales\|Streptococcaceae	2.710		0.1215	0.5717	0.4447	0.3174
Firmicutes\|Bacilli\|Lactobacillales\|Streptococcaceae\|Streptococcus	2.710		0.1215	0.5717	0.4447	0.3174
Firmicutes\|Erysipelotrichia\|Erysipelotrichales\|Erysipelotrichaceae\|Unclassified	3.097		-0.4944	0.0140¹	-0.1429	0.7825
Firmicutes\|Negativicutes\|Selenomonadales\|Unclassified	2.396		-0.2047	0.3374	0.7027	0.0782
Firmicutes\|Negativicutes\|Selenomonadales\|Unclassified\|Unclassified	2.396		-0.2047	0.3374	0.7027	0.0782
Fusobacteria	2.766	MSn = 10	0.4564	0.0250¹	0.2067	0.5667
Fusobacteria\|Fusobacteria	2.766		0.4564	0.0250¹	0.2067	0.5667
Fusobacteria\|Fusobacteria\|Fusobacteriales	2.766		0.4564	0.0250¹	0.2067	0.5667
Fusobacteria\|Fusobacteria\|Fusobacteriales\|Fusobacteriaceae	2.766		0.4564	0.0250¹	0.2067	0.5667
Fusobacteria\|Fusobacteria\|Fusobacteriales\|Fusobacteriaceae\|Fusobacterium	2.766		0.4564	0.0250¹	0.2067	0.5667
Proteobacteria\|Gammaproteobacteria	4.622		0.1809	0.3976	0.0667	0.8648
Proteobacteria\|Gammaproteobacteria\|Aeromonadales	4.622		0.1757	0.4115	0.0667	0.8648
Proteobacteria\|Gammaproteobacteria\|Aeromonadales\|Succinivibrionaceae	4.622		0.1757	0.4115	0.0667	0.8648
Proteobacteria\|Gammaproteobacteria\|Aeromonadales\|Succinivibrionaceae\|Anaerobiospirillum	4.622		0.1757	0.4115	0.0667	0.8648
Bacteroidetes\|Bacteroidia\|Bacteroidales\|Porphyromonadaceae\|Barnesiella	3.607	pTNBSn = 7	-0.1501	0.4840	-0.0180	0.9694
Firmicutes\|Clostridia\|Clostridiales\|Peptostreptococcaceae	3.776		0.1946	0.3623	0.0180	0.9694
Firmicutes\|Clostridia\|Clostridiales\|Peptostreptococcaceae\|Clostridium_XI	3.776		0.1946	0.3623	0.0180	0.9694
Firmicutes\|Clostridia\|Clostridiales\|Ruminococcaceae\|Unclassified	5.014		-0.0322	0.8813	-0.1982	0.6701

¹The P-value is less than 0.05. LDA: Linear discriminant analysis; MS: Maternal separation; pTNBS: TNBS post-inflammatory.

Citation: Zhou XY, Li M, Li X, Long X, Zuo XL, Hou XH, Cong YZ, Li YQ. Visceral hypersensitive rats share common dysbiosis features with irritable bowel syndrome patients. World J Gastroenterol 2016; 22(22): 5211-5227
URL: https://www.wjgnet.com/1007-9327/full/v22/i22/5211.htm
DOI: https://dx.doi.org/10.3748/wjg.v22.i22.5211