Hepatocyte transplantation program: Lessons learned and future strategies

Table 1 Hepatocyte isolation from whole organs or split liver grafts unsuitable for conventional liver transplantation for severity of the steatosis

Sample	Weight (g)	Age (yr)	Sex	Ischemia time (h)	Steatosis (%)	Viability (%)	Yield (× 106 cells/g)
LL	350	80	F	12	< 30	73	2.8
WL	3500	65	F	16	40-50	85	1.6
WL	1142	69	F	13	30	85	2.6
WL	2200	75	M	9	40	53	1.0
RL	1200	32	M	11	< 30	86	2.6
WL	2032	26	M	17	50	20	Discarded
WL	1550	58	M	15	40	20	0.11
WL	2354	67	M	6	10	20	Discarded

LL: Left lobe; RL: Right lobe; WL: Whole liver.

Table 2 Hepatocyte isolation from organs unsuitable for liver transplantation

Liver number	Sample	Weight (g)	Blood type	Age (yr)	Sex	Viability1 (%)	Yield (× 106 cells/g)	V (%)	Non-valid
1	LL	420	O	53	F	67	1.3	78	Split
2	WL	420	O	1	M	68.5	6	75	DS
3	RL	774	O	14	F	74.7	6	75	Split
4	LL	260	O	83	M	63.3	6.7	89	Liver fibrosis
5	LL	350	O	32	F	50	4.9	79.3	Liver fibrosis
	RL	832
	IV	676
6	LL	376	A	52	M	37.8	9.9	87.5	DCD
	RL	1154
7	LL	460	O	15	F	63.8	5.75	86.5	DCD
	RL	1162
8	LL	444	AB	73	M	NA	3.3	70	Liver fibrosis
9	LL	325	A	76	F	NA	5.25	88.5	AE
	RL	782
10	LL	488	O	82	M	41.7	4.95	80.5	AE
	RL	1140
11	RL	1292	O	15	F	71	8.7	87	Trauma,split
12	WL	238	A	4d	F	74	17	100	No recipient
13	RL	998	O	53	F	67	6.58	95	CAA
	LL	306

¹Viability after thawing. AE: Arteriosclerosis; CAA: Coeliac artery aneurism; DCD: Donation after circulatory death; DS: Doubt ful serology; LL: Left lobe; RL: Right lobe; V: Viability; WL: Whole liver.