Apoptosis of human gastric carcinoma cells induced by Euphorbia esula latex

Table 1 Primer sequences of Bax, Bcl-2 and β-actin genes

Gene	Forward primer	Backward primer
Bax	TTTGCTTCAGGGTTTCATCC	GGAGGAAGTCCAATGTCCAG
Bcl-2	GGATGCCTTTGTGGAACTGT	ATTCGACGTTTTGCCTGAAG
β-actin	GAAAATCTGGCCACCCACCT	GGGGTGTTGAAGGTCTCAAA

Table 2 Inhibition of SGC-7901 cells by Euphorbia esula extract

E. esula(mg/L)	OD490 (mean ± SD)		Inhibition rate (%)
	24 h	48 h	24 h	48 h
0 (control)	0.827 ± 0.020	0.818 ± 0.021	0	0
5	0.728 ± 0.018b	0.712 ± 0.020b	11.97b	12.96b
10	0.645 ± 0.020bd	0.620 ± 0.019bd	22.00bd	24.21bd
20	0.574 ± 0.019bd	0.501 ± 0.018bcd	30.59bd	38.75bcd
40	0.447 ± 0.017bd	0.397 ± 0.015bcd	45.95bd	51.47bcd
80	0.338 ± 0.021bd	0.274 ± 0.017bcd	59.13bd	66.50bcd
160	0.269 ± 0.019bd	0.214 ± 0.016bcd	67.47bd	73.84bcd

^bP < 0.01 vs control;

^dP < 0.01 vs previous concentration;

^cP < 0.05 vs 24 h treatment. n = 8. E. esula: Euphorbia esula.