Hepatitis B virus infection and genotype in asymptomatic people from 10 ethnic groups in Yunnan, China

Table 1 The primer sequences for identifying the hepatitis B virus genotypes/subgenotypes

Gene identification	PCR	Primer	Primer sequence	Site
Genotype	1st PCR	P1 (s)	5’-TCA CCA TAT TCT TGG GAA CAA GA-3’	nt2823–2845
(A-F)		S1-2 (as)	5’-CGA ACC ACT GAA CAA ATG GC -3’	nt685–704
	2nd PCR	B2 (s)	5’-GGC TCM AGT TCM GGA ACA GT-3’	nt67–86
	mixA	BA1R (as)	5’-CTC GCG GAG ATT GAC GAG ATG T-3’	nt113–134
		BB1R (as)	5’-CAG GTT GGT GAG TGA CTG GAG A-3’	nt324–345
		BC1R (as)	5’-GGT CCT AGG AAT CCT GAT GTT G-3’	nt 165–186
	mixB	BD1 (s)	5’-GCC AAC AAG GTA GGA GCT-3’	nt 2979–2996
		BE1 (s)	5’-CAC CAG AAA TCC AGA TTG GGA CCA-3’	nt 2955–2978
		BF1 (s)	5’-GYT ACG GTC CAG GGT TAC CA-3’	nt 3032–3051
		B2R (s)	5’-GGA GGC GGA TYT GCT GGC AA-3’	nt 3078–3097
Subgenotype	1st PCR	PC1 (s)	5’-CAT GCA ACT TTT TCA CCT CTG CCT-3’	nt1813-1836
B (B1 and B2)	2nd PCR	PC2 (s)	5’-ATT AGA CCT ATT GAT TGG AAA GT-3’	nt1861-1881
		COR-HBV (as)	5’-GAG TGC GAA TCC ACA CTC CA-3’	nt2285-2266
C (C1 and C2)	1st PCR	HBV964F (s)	5’-ATT AGA CCT ATT GAT TGG AAA GT-3’	nt964-986
	2nd PCR	HBV970F2 (s)	5’-CCT ATT GAT TGG AAA GTA TGT CA-3’	nt970-992
		HBV1272R (as)	5’-AGT ATG GAT CGG CAG AGG AG-3’	nt1272-1253

(s): Sense; (as): Anti-sense; M: A or C; Y: C or T; HBV: Hepatitis B virus; PCR: Polymerase chain reaction.

Table 2 Hepatitis B surface antigen-positive rates in the 10 ethnic groups in Yunnan Province

Ethnic group	Number	Age	Origin1	Gender		HBsAg
				Male n (%)	Female (%)	Positive	Rate n (%)
Han	165	16.01 ± 14.47	-	87 (52.7)	78 (47.3)	7	4.2
Dai	459	38.41 ± 18.57	S	159 (34.6)	300 (65.4)	43	9.42
Bulang	100	36.24 ± 18.11	S	54 (54.0)	46 (46.0)	7	7.0
Pumi	61	34.90 ± 18.44	S	33 (54.1)	28 (45.9)	15	24.62
Achang	347	30.19 ± 18.46	N	154 (44.4)	193 (55.6)	16	4.6
Mosuo	234	31.61 ± 18.14	N	101 (43.0)	133 (57.0)	15	6.42
Naxi	196	8.78 ± 18.32	N	100 (51.0)	96 (49.0)	2	1.0
Dulong	305	31.51 ± 18.66	N	143 (46.9)	162 (53.1)	10	3.3
Bai	401	32.78 ± 17.12	N	178 (44.4)	223 (55.6)	22	5.5
Lisu	316	18.24 ± 18.02	N	144 (45.6)	172 (54.4)	10	3.2
Total	2584	28.35 ± 18.66		1153 (44.6)	1431 (55.4)	147	5.7

¹Historical ethnic origin;

²Indicates those with higher than average values. N: From north China; S: From south China; -: Han has no specific origin; HBsAg: Hepatitis B surface antigen.

Table 3 The distribution of the genotypes and subgenotypes in different ethnic groups

Ethic group	Total	Source1	Genotype				Subgenotype
			B	C	B + C	Sum	Ba	Cs	Ce
Han	165	-	1	0	1	2	1	0	0
Dai	459	S	13	4	1	18	13	3	0
Bulang	100	S	0	0	1	1	0	1	0
Pumi	61	S	4	0	0	4	3	0
Achang	347	N	2	0	4	6	3	1	0
Mosuo	234	N	3	3	1	7	0	0	0
Naxi	196	N	1	0	0	1	1	0	0
Dulong	305	N	1	1	0	2	1	1	0
Bai	401	N	6	3	2	11	4	3	0
Lisu	316	N	3	1	1	5	0	1	1

¹Historical ethnic origin. N: From north China; S: From south China; -: Han has no specific origin.

Table 4 The distribution of the hepatitis B virus genotypes/subgenotypes in different serum marker models

Serum marker mode	Genotype				Subgenotype
	B	C	B + C	Subtotal	Ba	Cs	Ce	Subtotal
HBsAg+HBeAg+HBcAb+	22	6	6	34	15	7	1	23
HBsAg+HBeAb+HBcAb+	7	4	2	13	6	3	0	9
HBsAg+HBcAb+	3	0	3	6	2	1	0	3
HBeAg+	2	2	0	4	1	1	0	2
Total	34	12	11	57	24	12	1	37

HBsAg: Hepatitis B surface antigen; HBeAg: Hepatitis B e antigen; HBcAb: Hepatitis B core antibody; HBeAb: Hepatitis B e antibody.