Magnesium isoglycyrrhizinate inhibits inflammatory response through STAT3 pathway to protect remnant liver function

Table 1 Serological test results of experimental animals after excessive liver resection

		Time point (mean ± SD)
Item	Group	0 h	6 h	12 h	18 h	P value1
	Control	165.8 ± 118.0	864.0 ± 471.7	1927.5 ± 1079.0	1969.6 ± 1201.6	0.000
ALT (U/L)	Low dose	86.4 ± 23.4	357.2 ± 119.8	705.1 ± 341.5	1597.7 ± 998.6	0.000
	High dose	145.6 ± 106.8	524.7 ± 182.5	1234.8 ± 784.2	1321.2 ± 797.4	0.000
	P value2	0.133	0.002	0.003	0.3
	Control	228.4 ± 110.4	929.3 ± 477.7	1779.5 ± 495.9	1673.2 ± 668.8	0.000
AST (U/L)	Low dose	192.6 ± 37.5	664.7 ± 171.5	1027.5 ± 396.9	1904.3 ± 794.7	0.000
	High dose	193.1 ± 99.5	694.6 ± 278.7	1370.6 ± 502.7	1355.2 ± 671.5	0.000
	P value	0.556	0.158	0.001	0.192
	Control	1.11 ± 0.93	1.50 ± 0.85	2.14 ± 1.51	1.55 ± 0.93	0.197
GGT (U/L)	Low dose	0.70 ± 0.48	0.88 ± 0.35	1.27 ± 1.27	2.89 ± 2.42	0.008
	High dose	0.72 ± 0.47	1.00 ± 0.71	2.36 ± 1.21	2.00 ± 1.08	0.001
	P value	0.316	0.154	0.147	0.158
	Control	50.7 ± 4.9	47.3 ± 3.6	46.3 ± 4.8	45.5 ± 3.6	0.031
TP (g/L)	Low dose	48.3 ± 2.9	45.0 ± 2.9	44.9 ± 3.2	44.6 ± 3.8	0.039
	High dose	51.6 ± 3.3	45.5 ± 2.4	48.5 ± 3.6	45.6 ± 5.8	0.001
	P value	0.101	0.212	0.094	0.849
	Control	1.95 ± 0.62	6.68 ± 1.32	16.59 ± 2.16	20.13 ± 4.19	0.000
TBil (U/L)	Low dose	1.20 ± 0.30	4.35 ± 0.77	7.45 ± 4.66	14.82 ± 2.06	0.000
	High dose	1.67 ± 0.43	6.23 ± 2.22	14.31 ± 2.16	12.81 ± 2.29	0.000
	P value	0.539	0.532	0.011	0.206
	Control	1.22 ± 0.37	4.27 ± 0.64	13.56 ± 1.83	16.33 ± 3.46	0.000
DBil (U/L)	Low dose	0.65 ± 0.15	2.88 ± 0.47	5.25 ± 1.01	11.15 ± 1.72	0.000
	High dose	0.99 ± 0.26	4.62 ± 1.82	10.95 ± 1.59	9.72 ± 1.80	0.000
	P value	0.353	0.527	0.004	0.14
	Control	7.41 ± 1.27	4.92 ± 2.54	6.83 ± 2.33	4.21 ± 1.40	0.001
Glu (mmol/L)	Low dose	6.50 ± 1.21	6.30 ± 1.62	6.09 ± 1.84	4.11 ± 2.13	0.012
	High dose	7.12 ± 2.05	8.54 ± 3.69	5.25 ± 2.19	5.21 ± 1.02	0.002
	P value	0.395	0.016	0.162	0.149
	Control	42.7 ± 6.1	42.0 ± 4.6	47.8 ± 10.1	41.2 ± 10.0	0.754
TT (s)	Low dose	48.9 ± 3.5	49.7 ± 6.6	49.5 ± 5.3	48.4 ± 0.4	0.984
	High dose	38.2 ± 6.1	46.2 ± 2.0	11.5 ± 1.1	43.2 ± 1.1	0.000
	P value	0.127	0.226	0.001	0.36
	Control	8.9 ± 0.6	10.3 ± 0.3	11.7 ± 1.1	12.4 ± 0.4	0.001
PT (s)	Low dose	9.9 ± 0.6	10.9 ± 0.5	12.6 ± 1.9	14.2 ± 1.6	0.016
	High dose	9.1 ± 0.1	12.6 ± 0.7	11.5 ± 1.1	14.8 ± 0.8	0.000
	P value	0.077	0.003	0.618	0.076

¹Comparison among time points of same group;

²Comparison among groups at same time point. ALT: Alanine aminotransferase; AST: Aspartate aminotransferase; PT: Prothrombin time; TT: Thrombin time; TBil: Total bilirubin; Glu: Glucose; DBil: Direct bilirubin.

Table 2 STAT3 mRNA levels at various time points after excessive liver resection in rats

	STAT3 mRNA expression (mean ± SD)
Group	0 h	n	6 h	n	12 h	n	18 h	n	P value1
Control	0.33 ± 0.11	3	0.43 ± 0.21	3	0.57 ± 0.24	3	1.07 ± 0.47	3	0.021
Low dose	0.41 ± 0.15	3	0.44 ± 0.19	3	0.78 ± 0.34	3	0.89 ± 0.45	3	0.043
High dose	0.38 ± 0.24	3	0.11 ± 0.07	3	0.30 ± 0.14	3	0.87 ± 0.36	3	0.025
P value2	0.401		0.012		0.044		0.849

¹Comparison among time points of same group;

²Comparison among groups at same time point.