Influence of the hTERT rs2736100 polymorphism on telomere length in gastric cancer

Table 1 The Primer sequences used in this study

Gene	Primer sequences
hTERT rs2736100	F: 5’-GGT GCC TCC AGA AAA GCA G-3’
	R: 5’-GAC ACG GAT CCA GGA CCT C-3’
Telomere standard	5’-TTA GGG TTA GGG TTA GGG TTA GGG TTA GGG TTA GGG TTA GGG TTA GGG TTA GGG TTA GGG TTA GGG TTA GGG TTA GGG TTA GGG-3’
36B4 standard	5’-CAG CAA GTG GGA AGG TGT AAT CCG TCT CCA CAG ACA AGG CCA GGA CTC GTT TGT ACC CGT TGA TGA TAG AAT GGG-3’
Telomere	F: 5’-CGG TTT GTT TGG GTT TGG GTT TGG GTT TGG GTT TGG GTT-3’
	R: 5’-GGC TTG CCT TAC CCT TAC CCT TAC CCT TAC CCT TAC CCT-3’
36B4	F: 5’-CAG CAA GTG GGA AGG TGT AAT CC-3’
	R: 5’-CCC ATT CTA TCA TCA ACG GGT ACA A-3’
hTERT	F: 5’-ATG CGA CAG TTC GTG GCT CA-3’
	R: 5’-ATC CCC TGG CAC TGG ACG TA-3’
GAPDH	F: 5’- AAA TCA AGT GGG GCG ATG CTG-3’
	R: 5’- GCA GAG ATG ATG ACC CTT TTG-3’

GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; hTERT: Human telomerase reverse transcriptase.

Table 2 Genotype and allele distribution of rs2736100 gene polymorphism in gastric cancer patients and controls

	Patients(n = 243),n (%)	Controls(n = 246),n (%)	Crude OR (95%CI)	Adjusted1 OR (95%CI)
CC	102 (42.0)	86 (35.0)	1.00	1.00
CA	107 (44.0)	122 (49.6)	0.739 (0.502-1.089)	0.741 (0.447-1.228)
AA	34 (14.0)	38 (15.4)	0.754 (0.438-1.300)	0.811 (0.373-1.760)
C:A allele frequency2	311:175 (0.640)	294:198 (0.598)
Trend test3			P = 0.1501	P = 0.3454

¹Adjusted for age (in year) and sex;

²Two-sided χ² test: for allele frequencies, P = 0.1727; for genotype distribution, P = 0.2797;

³Calculated in the logistic regression model using the number of A alleles in the genotypes as a continuous variable.

Table 3 Genotype and allele distribution of rs2736100 gene polymorphism in gastric cancer patients according to histopathology

	Patients (n = 243)			Controls (n = 246)			Adjusted1 OR (95%CI)	Adjusted1 OR (95%CI)
	CC	CA	AA	CC	CA	AA	CA vs CC	AA vs CC
Age (yr)
≤ 50	14	20	1	72	98	33	1.053 (0.498-2.226)	0.229 (0.039-1.351)
> 50	88	87	33	14	24	5	0.568 (0.272-1.184)	1.029 (0.342-3.101)
Gender
M	72	77	25	49	63	23	0.871 (0.452-1.679)	1.070 (0.392-2.923)
F	30	30	9	37	59	15	0.582 (0.262-1.293)	0.574 (0.162-2.031)
Lauren’s
Diffuse	37	46	15	P = 0.5448
Intestinal	65	61	19	(χ2 test)

¹Adjusted for the other covariates [age (in years) as a continuous variable] presented in this table in a logistic regression model for each stratum.