Basic Study
Copyright ©The Author(s) 2015.
World J Gastroenterol. Aug 14, 2015; 21(30): 9079-9092
Published online Aug 14, 2015. doi: 10.3748/wjg.v21.i30.9079
Table 1 Oligonucleotide primers for real-time reverse transcription polymerase chain reaction
GenePrimerSequence (5’-3’)PCR product (bp)
β-actinForwardTGTTACCAACTGGGACGACA165
(NM_007393.3)ReverseGGGGTGTTGAAGGTCTCAAA
TNF-αForwardTAGCCAGGAGGGAGAACAGA127
(NM_013693.2)ReverseTTTTCTGGAGGGAGATGTGG
IL-6ForwardTCCAGTTGCCTTCTTGGGAC140
(NM_031168.1)ReverseGTGTAATTAAGCCTCCGACTTG
BaxForwardCTGCAGAGGATGATTGCTGA174
(NM_007527.3)ReverseGATCAGCTCGGGCACTTTAG
Bcl-2ForwardGGACTTGAAGTGCCATTGGT127
(NM_177410.2)ReverseAGCCCCTCTGTGACAGCTTA
Caspase-3ForwardATGGGAGCAAGTCAGTGGAC137
(NM_009810.2)ReverseCGTACCAGAGCGAGATGACA