Overexpression of pim-3 and protective role in lipopolysaccharide-stimulated hepatic stellate cells

Table 1 Effect of lipopolysaccharide on hepatic stellate cells-T6 proliferation assessed by methyl thiazoyltetrazolium assay

Parameter	24 h (OD)	48 h (OD)
Control	0.5033 ± 0.023	0.8853 ± 0.021
LPS (10 ng/mL)	0.5998 ± 0.019ab	0.9813 ± 0.037ab
LPS (100 ng/mL)	0.6002 ± 0.038ab	1.1126 ± 0.050ab
LPS (1000 ng/mL)	0.7032 ± 0.066b	1.2469 ± 0.0525b
LPS (5000 ng/mL)	0.6625 ± 0.015b	1.2141 ± 0.018b

Data are expressed as mean ± SD (n = 6).

^aP < 0.05 vs LPS, 1000 ng/mL,

^bP < 0.01 vs control.

Table 2 Effect of ablation of pim-3 on HSC-T6 cell proliferation assessed by methyl thiazoyltetrazolium assay

Group	24 h (OD)	48 h (OD)
Control	0.5267 ± 0.030	0.8435 ± 0.028
Liposomes	0.5749 ± 0.028	0.8552 ± 0.014
si-pim3	0.2987 ± 0.050b	0.4634 ± 0.056b
scrambled	0.5062 ± 0.066	0.8069 ± 0.039
si-pim3 plus LPS	0.4063 ± 0.051ab	0.5434 ± 0.031b
LPS	0.7435 ± 0.028b	1.2136 ± 0.048bc

Data are expressed as mean ± SD (n = 6).

^aP < 0.05 vs si-pim3 group;

^bP < 0.01 vs control group;

^cP < 0.01 LPS vs other groups.

Table 3 Effect of ablation of pim-3 on caspase-3 activity of hepatic stellate cells-T6 cells

Groups	Caspase-3 activity (U)
Control	5.12 ± 1.33
Liposomes	4.95 ± 1.62
si-pim3	13.24 ± 2.81bd
scrambled	5.28 ± 0.93
si-pim3 plus LPS	11.35 ± 0.85bd
LPS	3.21 ± 0.55a

Data are expressed as mean ± SD (n = 6).

^aP < 0.05 vs control group;

^bP < 0.01 vs control group;

^dP < 0.01 vs LPS.