Proportions of acetyl-histone-positive hepatocytes indicate the functional status and prognosis of cirrhotic patients

Table 1 Information regarding the detected antibodies in this study

Antibody	Clone	Vendor	Stain location
H2AK5ac	ab45152 rabbit monoclonal	Abcam	Nuclear
H2BK5ac	Ab40886 rabbit monoclonal	Abcam	Nuclear
H3K9/K14ac	06-599 rabbit polyclonal	Millipore	Nuclear
H3K18ac	ab40888 rabbit monoclonal	Abcam	Nuclear
H3K23ac	ab61234 rabbit polyclonal	Ancam	Nuclear
H3K27ac	ab4729 rabbit polyclonal	Abcam	Nuclear

Table 2 Indicator differences between the 152 follow-up cases and all 611 cases

	Follow-up (n = 152)	Total (n = 611)	P value
Age (yr)	46.52 ± 12.07	44.74 ± 11.81	0.099
Total bilirubin (μmol/L)	35.29 ± 41.09	36.72 ± 39.18	0.182
Albumin (g/dL)	3.73 ± 0.62	3.69 ± 0.64	0.497
Prothrombin time (INR)	5.21 ± 6.58	6.21 ± 7.11	0.002
Activated partial thromboplastin time (s)	44.72 ± 15.97	43.87 ± 14.20	0.862
Alanine aminotransferase (IU/L)	48.20 ± 28.95	52.06 ± 41.06	0.164
Aspartate aminotransferase (IU/L)	59.21 ± 46.12	64.59 ± 47.92	0.060
γ-glutamyl transpeptidase (IU/L)	72.55 ± 61.74	72.76 ± 59.84	0.630
Creatine kinase (IU/L)	78.49 ± 64.05	84.79 ± 73.36	0.266
Lactic dehydrogenase (IU/L)	183.01 ± 66.27	187.78 ± 62.43	0.203
Alpha fetoprotein (μg/L)	23.95 ± 52.26	29.22 ± 67.36	0.464

Mann-Whitney U Test.

Table 3 Univariate and multivariate analyses of risk factors for patient survival

Variable	Unitivariate analysis			Multivariate analysis
	OS (%)	95%CI	P value	HR	95%CI	P value
Age (yr)
< 60	75.0	66.2-83.8	0.035	0.35	0.12-1.04	0.059
> 60	49.4	24.1-74.7
Gender					0.858-6.009
Female	75.1	59.0-91.2	0.171	2.27		0.099
Male	69.0	59.2-78.8
CTP grade					0.26-8.56
A, B	72.0	153.8-179.7	0.041	1.36		0.744
C	50.0	10.0-89.9
Ascites					0.31-2.60	0.843
No	71.8	62.2-81.4	0.535	0.90
Yes	68.5	50.9-86.1
H2AK5ac					1.68-24.49
< 70%	94.7	88.6-100.8	0.000	6.41		0.007
> 70%	50.3	34.6-66.0
H3K9/K14ac				1.88	0.67-5.42
< 70%	83.4	72.2-94.6	0.000			0.240
> 70%	56.9	44.6-69.2
H3K27ac
< 70%	86.4	79.1-93.7	0.000	4.75	1.82-12.36	0.001
> 70%	40.4	22.9-57.8

OS: Overall survival; HR: Hazard ratio.