Butein effects in colitis and interleukin-6/signal transducer and activator of transcription 3 expression

Table 1 Histologic scores

Grade 0	Normal tissue
Grade 1	One or a few multifocal mononuclear cell infiltrates in the lamina propria
	Minimal epithelial hyperplasia
	Slight to no depletion of mucus from goblet cells
Grade 2	Lesions tended to involve more of the intestine than grade 1 lesions
	Several multifocal, mild inflammatory cell infiltrates in the lamina propria
	Mild epithelial hyperplasia and mucin depletion
	Small epithelial erosions
Grade 3	Lesions involved a large area of the mucosa or were more frequent than grade 2 lesions
	Moderate inflammation with the involvement of submucosa
	Mixture of mononuclear cells as well as neutrophils, and crypt abscesses
	Moderate epithelial hyperplasia and mucin depletion
	Ulcers were occasionally observed
Grade 4	Lesions usually involved most of the intestinal section and were more severe than grade 3 lesions
	Severe inflammation including mononuclear cells and neutrophils with transmural involvement
	Marked epithelial hyperplasia
	Crypt abscesses and ulcers

Table 2 Primer list

Name		Sequence
IL-6	F	AGT TGC CTT CTT GGG ACT GA
	R	TCC ACG ATT TCC CAG AGA AC
IL-1b	F	GCC CAT CCT CTG TGA CTC AT
	R	AGG CCA CAG GTA TTT TGT CG
IL-22	F	CAA CTT CCA GCA GCC ATA CA
	R	GTT GAG CAC CTG CTT CAT CA
IL-17A	F	TCC AGA AGG CCC TCA GAC TA
	R	AGC ATC TTC TCG ACC CTG AA
IFN-γ	F	ACT GGC AAA AGG ATG GTG AC
	R	TGA GCT CAT TGA ATG CTT GG
MMP-2	F	CAG ACT TGT CCC GTT TCC AT
	R	GGT GCT GAC TGC ATC AAA GA
MMP-9	F	CGT CGT GAT CCC CAC TTA CT
	R	AAC ACA CAG GGT TTG CCT TC
IL-21	F	GGC AAC ATG GAG AGG ATT GT
	R	AAG CAG GAA AAA GCT GAC CA
IL-23R	F	CAT GAC TTG CAC CTG GAA TG
	R	GCT TGG ACC CAA ACC AAG TA
IL-12β (P.40)	F	AGG TCA CAC TGG ACC AAA GG
	R	TGG TTT GAT GAT GTC CCT GA

IFN: Interferon; IL: Interleukin; MMP: Matrix metalloproteinase; F: Forward; R: Reverse.