MicroRNA-185-5p mediates regulation of SREBP2 expression by hepatitis C virus core protein

Table 1 Primers used for mutagenesis

Mutant binding site	5'-3'
a	Forward	CCTTCCTGAGTTTCGCGCGCCTGAACCCTACTCTC
	Reverse	GAGAGTAGGGTTCAGGCGCGCGAAACTCAGGAAGG
b	Forward	CTCTCCTGAACCCTACGCGCGCCTTTTTGCTTCCTC
	Reverse	GAGGAAGCAAAAAGGCGCGCGTAGGGTTCAGGAGAG
c	Forward	CTGTAGCGTCTTGATGCGCGCCCTGGGTCTGCG
	Reverse	CGCAGACCCAGGGCGCGCATCAAGACGCTACAG
d	Forward	CTCTCTCTCGATTTCTCGCGCGCCCCCTCAGCATC
	Reverse	GATGCTGAGGGGGCGCGCGAGAAATCGAGAGAGAG

“G” denotes mutation sites.

Table 2 Predicted miRNAs to target sterol regulatory element binding protein 2 by target prediction algorithms

DIANAmicroT_v3_targets	Targetscan		Microrna. org	miRDB	PITA
Has-miR-98	24	4644	542-3p	4279	423-5p	1275
124	185	1193-5p	24	4306	185	1303
185	193	125a-3p	185	4644	298	1207-5p
506	370	193-3p	543	149-3p	346	1207-5p
650	379	193b	874	185-5p	448	296-3p
940	539	24-3p		1915-3p	608	296-5p
331-3p	543	24ab		4446-3p	612	664b
532-3p	544	296-3p		4628-3p	637	671-5p
let-7a	873	324-5p			638
let-7b	874	539-5p			650
let-7c	882	542-3p			877
let-7e	1554	544-3p			921
let-7f	3473	544ab			939
let-7g	3529				1268
let-7i	4306				1269

miRNA: MicroRNA; SREBP2: Sterol regulatory element binding protein 2.