Copyright
©The Author(s) 2015.
World J Gastroenterol. Apr 14, 2015; 21(14): 4225-4231
Published online Apr 14, 2015. doi: 10.3748/wjg.v21.i14.4225
Published online Apr 14, 2015. doi: 10.3748/wjg.v21.i14.4225
Table 1 Resistance of multidrug resistance strains to the five antibiotics
No. | Levofloxacin | Amoxicillin | Clarithromycin | Tetracycline | Metronidazole |
23 | R | R | R | R | R |
40 | R | R | R | R | R |
41 | S | R | R | R | R |
42 | R | R | I | R | R |
Table 2 Minimum inhibitory concentrations of the four Chinese medicines against multidrug resistance strains (mg/mL)
No. | Emodin | Berberine | Berberine | Schizandrin |
23 | 12.5 | 50 | 200 | 50 |
40 | 12.5 | 100 | 100 | 100 |
41 | 12.5 | 100 | 100 | 100 |
42 | 12.5 | 25 | 100 | 50 |
Table 3 Minimum inhibitory concentrations of amoxicillin against the four multidrug resistance strains after treatment with the four traditional Chinese medicines (μg/mL)
No. | Emodin | Berberine | Berberine | Schizandrin | Pantoprazole | NC |
23 | 32 | 16 | 64 | 32 | 32 | 64 |
40 | 16 | 8 | 16 | 16 | 16 | 32 |
41 | 64 | 64 | 21 | 64 | 32 | 64 |
42 | 32 | 16 | 32 | 32 | 32 | 32 |
Table 4 Minimum inhibitory concentrations of tetracycline against the four multidrug resistance strains after treatment with the four traditional Chinese medicines (μg/mL)
No. | Emodin | Berberine | Berberine | Schizandrin | Pantoprazole | NC |
23 | 64 | 32 | 64 | 64 | 32 | 64 |
40 | 16 | 16 | 32 | 32 | 32 | 32 |
41 | 32 | 32 | 32 | 64 | 16 | 32 |
42 | 32 | 16 | 32 | 32 | 32 | 32 |
Table 5 Minimum inhibitory concentrations of metronidazole against the four multidrug resistance strains after treatment with the four traditional Chinese medicines (μg/mL)
No. | Emodin | Berberine | Berberine | Schizandrin | Pantoprazole | NC |
23 | 128 | 128 | 128 | 128 | 64 | 128 |
40 | 64 | 64 | 64 | 64 | 32 | 64 |
41 | 64 | 64 | 64 | 64 | 32 | 64 |
42 | 64 | 64 | 64 | 64 | 64 | 64 |
Table 6 Minimum inhibitory concentrations of clarithromycin against the four multidrug resistance strains after treatment with the four traditional Chinese medicines (μg/mL)
No. | Emodin | Berberine | Berberine | Schizandrin | Pantoprazole | NC |
23 | 64 | 64 | 64 | 64 | 32 | 64 |
40 | 32 | 32 | 32 | 32 | 16 | 32 |
41 | 32 | 32 | 32 | 32 | 16 | 32 |
42 | 32 | 32 | 32 | 32 | 32 | 32 |
Table 7 Minimum inhibitory concentrations of levofloxacin against the four multidrug resistance strains after treatment with the four traditional Chinese medicines (μg/mL)
No. | Emodin | Berberine | Berberine | Schizandrin | Pantoprazole | NC |
23 | 64 | 64 | 64 | 64 | 32 | 64 |
40 | 32 | 32 | 32 | 32 | 16 | 32 |
41 | 32 | 32 | 32 | 32 | 16 | 32 |
42 | 64 | 64 | 64 | 64 | 64 | 64 |
Table 8 Real-time PCR quantitative results for hefA gene expression in 16 sub-cultured strains with significantly changed minimum inhibitory concentration of amoxicillin
No. | hefA | 16S rRNA | ∆Ct | ∆∆Ct | 2-(∆∆Ct) |
1 | 31.0933170 | 14.457372 | 16.63595 | 0 | 1 |
2 | 30.2871494 | 15.427569 | 14.85958 | -1.77637 | 3.425620 |
3 | 29.8203506 | 16.133926 | 13.68642 | -2.94952 | 7.724926 |
4 | 30.2641807 | 16.449774 | 13.81441 | -2.82154 | 7.069158 |
5 | 33.8933296 | 15.226003 | 18.66733 | 2.031382 | 0.244621 |
6 | 32.0969762 | 15.256840 | 16.84014 | 0.204191 | 0.868025 |
7 | 29.9665985 | 15.307595 | 14.65900 | -1.97694 | 3.936578 |
8 | 31.2955170 | 14.817378 | 16.47814 | -0.15781 | 1.115590 |
9 | 31.6979313 | 14.758531 | 16.93940 | 0.303455 | 0.810309 |
10 | 30.7486935 | 14.488201 | 16.26049 | -0.37545 | 1.297247 |
11 | 34.3626747 | 18.408577 | 15.95410 | -0.68185 | 1.604193 |
12 | 36.2199669 | 18.414414 | 17.80555 | 1.169608 | 0.444542 |
13 | 33.9337616 | 17.102884 | 16.83088 | 0.194932 | 0.873614 |
14 | 31.4263000 | 14.820235 | 16.60606 | -0.02988 | 1.020928 |
15 | 32.9468956 | 14.884178 | 18.06272 | 1.426772 | 0.371962 |
16 | 31.6464024 | 15.465043 | 16.18136 | -0.45459 | 1.370390 |
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Citation: Huang YQ, Huang GR, Wu MH, Tang HY, Huang ZS, Zhou XH, Yu WQ, Su JW, Mo XQ, Chen BP, Zhao LJ, Huang XF, Wei HY, Wei LD. Inhibitory effects of emodin, baicalin, schizandrin and berberine on
hefA gene: Treatment ofHelicobacter pylori -induced multidrug resistance. World J Gastroenterol 2015; 21(14): 4225-4231 - URL: https://www.wjgnet.com/1007-9327/full/v21/i14/4225.htm
- DOI: https://dx.doi.org/10.3748/wjg.v21.i14.4225