Copyright
©The Author(s) 2015.
World J Gastroenterol. Apr 14, 2015; 21(14): 4216-4224
Published online Apr 14, 2015. doi: 10.3748/wjg.v21.i14.4216
Published online Apr 14, 2015. doi: 10.3748/wjg.v21.i14.4216
Table 1 Basic data of the study population
Characteristics | Value |
Sex | |
Male | 33 |
Female | 17 |
Age (yr), mean (range) | 60 (38-81) |
Colon | 22 |
Rectum | 28 |
TNM stage | |
Stage I-II | 23 |
Stage III-IV | 27 |
Table 2 Primer sequences for polymerase chain reaction
Gene | Sequence (5’ to 3’) | Product (bp) | Tm (°C) |
IL-22 | F: GTTCTCCTTCCCCAGTCACCA | 145 | 60 |
R: AGCTGCTCCTCCCTGTACCAA | |||
AHR | F: ACATCACCTACGCCAGTCG | 94 | 60 |
R: CGCTTGGAAGGATTTGACTTGA | |||
CCL20 | F: ATCCAAAACAGACTTGGGTGAA | 89 | 60 |
R: TCCATTCCAGAAAAGCCACA | |||
CCL22 | F: ATTACGTCCGTTACCGTCTGC | 100 | 60 |
R: TCCCTGAAGGTTAGCAACACC | |||
CCL27 | F: TCCTGAGCCCAGACCCTACA | 175 | 60 |
R: CGTTGAGCCAGGTGAAGCA | |||
β-actin | F: TGACGTGGACATCCGCAAAG | 205 | 60 |
R: CTGGAAGGTGGACAGCGAGG |
- Citation: Huang YH, Cao YF, Jiang ZY, Zhang S, Gao F. Th22 cell accumulation is associated with colorectal cancer development. World J Gastroenterol 2015; 21(14): 4216-4224
- URL: https://www.wjgnet.com/1007-9327/full/v21/i14/4216.htm
- DOI: https://dx.doi.org/10.3748/wjg.v21.i14.4216