Copyright
©The Author(s) 2015.
World J Gastroenterol. Apr 7, 2015; 21(13): 3876-3887
Published online Apr 7, 2015. doi: 10.3748/wjg.v21.i13.3876
Published online Apr 7, 2015. doi: 10.3748/wjg.v21.i13.3876
Genes | Analysis | 5'-Sequence-3' |
FUT1 | RT-PCR | F: AGACTTTGCCCTGCTCACAC |
R: TGAAGTTGGCCAGGTAGACAG | ||
FUT2 | RT-PCR | F: CAGATGCCTTTCTCCTTTCC |
R: ACTCCCACATGGCTTGAATC | ||
FUT3 | RT-PCR | F: CTGGATCTGGTTCAACTTGG |
R: CGGTAGGACATGGTGAGATTG | ||
FUT4 | RT-PCR | F: GGGTTTGGATGAACTTCGAG |
R: AGCCATAAGGCACAAAGACG | ||
FUT8 | RT-PCR | F: ATCCTGATGCCTCTGCAAAC |
R: GGGTTGGTGAGCATAAATGG | ||
GMDS | RT-PCR | F: TGGAGGCTATGTGGTTGATG |
R: CAAATTCCCGGACACTATGG | ||
FX | RT-PCR | F: ACACGTCATCCATCTTGCTG |
R: AGGACGTTGTCGTTCATGTG | ||
SLC35C1 | RT-PCR | F: GGTGTGGCCTTCTACAATGTG |
R: ATGATGATACCGCAGGTGAG | ||
HNF4α | RT-PCR | F: CATCTTCTTTGACCCAGATGC |
R: CGTTGATGTAGTCCTCCAAGC | ||
IL-6 | RT-PCR | F: ATGCAATAACCACCCCTGAC |
R: GCGCAGAATGAGATGAGTTG | ||
RPL4 | RT-PCR | F: GACTTAACACACGAGGAGATGC |
R: GCATGCTGTGCACATTTAGG | ||
FUT1 | shRNA | GGTAATCAGATGGGACAGTAT |
Negative control | shRNA | GGAATCTCATTCGATGCATAC |
SLC35C1 | shRNA | CUACUUAUAGACCCAAUCATT |
Negative control | shRNA | UCUUAAUCGCGUAUAAGGCTT |
- Citation: Terao N, Takamatsu S, Minehira T, Sobajima T, Nakayama K, Kamada Y, Miyoshi E. Fucosylation is a common glycosylation type in pancreatic cancer stem cell-like phenotypes. World J Gastroenterol 2015; 21(13): 3876-3887
- URL: https://www.wjgnet.com/1007-9327/full/v21/i13/3876.htm
- DOI: https://dx.doi.org/10.3748/wjg.v21.i13.3876