Clinicopathologic and prognostic relevance of ARID1A protein loss in colorectal cancer

Table 1 Clinicopathologic characteristics and AT-rich interactive domain 1A expression in 209 patient samples of colorectal cancer n (%)

Characteristics	Value
Gender
Male	176 (84.2)
Female	33 (15.8)
Age (yr)
Median	55
Range	19-88
Tumor location
Colon	111 (53.1)
Rectal	98 (46.9)
TNM stage (AJCC)
I	35 (16.7)
II	48 (23.0)
III	55 (26.3)
IV	71 (34.0)
T stage
T1	10 (4.8)
T2	35 (16.7)
T3	53 (25.4)
T4	111 (53.1)
N stage
N0	98 (46.9)
N1	56 (26.8)
N2	55 (26.3)
M stage
M0	138 (66.0)
M1	71 (34.0)
Pathologic differentiation
Poor	36 (17.2)
Moderate	148 (70.8)
Well	25 (12.0)
Tumor size (cm)
≤ 5	136 (65.1)
> 5	73 (34.9)
Expression of ARID1a
Negative	54 (25.8)
Positive	155 (74.2)

AJCC: American Joint Committee on Cancer; TNM: Tumor-node-metastasis; ARID1A: AT-rich interactive domain 1A.

Table 2 Correlation between AT-rich interactive domain 1A expression and clinicopathologic characteristics of colorectal cancer patients n (%)

Characteristics	ARID1a		P value
	Negative	Positive
Gender			0.132
Male	42 (77.8)	134 (86.5)
Female	12 (22.2)	21 (13.5)
Age (yr)			0.409
≤ 55	30 (55.6)	76 (49.0)
> 55	24 (44.4)	79 (51.0)
Tumor location			0.293
Colon	32 (59.3)	79 (51.0)
Rectal	22 (40.7)	76 (49.0)
TNM stage (AJCC)			0.020
I	4 (7.4)	31 (20.0)
II	13 (24.1)	35 (22.6)
III	12 (22.2)	43 (27.7)
IV	25 (46.3)	46 (29.7)
T stage			0.771
T1	1 (1.9)	9 (5.8)
T2	6 (11.1)	29 (18.7)
T3	20 (37.0)	33 (21.3)
T4	27 (50.0)	84 (54.2)
N stage			0.638
N0	23 (42.6)	75 (48.4)
N1	17 (31.5)	39 (25.1)
N2	14 (25.9)	41 (26.5)
M stage			0.026
M0	29 (53.7)	109 (70.3)
M1	25 (46.3)	46 (29.7)
Pathologic differentiation			0.035
Poor	14 (25.9)	22 (14.2)
Moderate	36 (66.7)	112 (72.3)
Well	4 (7.4)	21 (13.5)
Tumor size (cm)			0.170
≤ 5	31 (57.4)	105 (67.7)
> 5	23 (42.6)	50 (32.3)

AJCC: American Joint Committee on Cancer; TNM: Tumor-node-metastasis; ARID1A: AT-rich interactive domain 1A.

Table 3 Univariate and multivariate analyses of various prognostic parameters in stage IV patients with colorectal cancer using Cox-regression analysis n (%)

Characteristics	Univariate analysis		Multivariate analysis
		P value	HR	95%CI	P value
Gender		0.258
Male	40 (56.3)
Female	31 (43.7)
Age (yr)		0.934
≤ 55	39 (54.9)
> 55	32 (45.1)
ARID1a expression		0.027	2.49	1.13-5.51	0.024
Negative	25 (35.2)
Positive	46 (64.8)
T stage		0.038	2.01	1.08-3.75	0.029
T1	1 (1.4)
T2	5 (7.0)
T3	43 (60.6)
T4	22 (31.0)
N stage		0.929
N0	12 (16.9)
N1	27 (38.0)
N2	32 (45.1)
Pathologic differentiation		0.458
Poor	17 (23.9)
Moderate	51 (71.8)
Well	3 (4.3)
Tumor size (cm)		0.261
≤ 5	43 (60.6)
> 5	28 (39.4)
Metastatic site		0.806
Single	52 (73.2)
Multiple	19 (26.8)
Metastasis limited to the liver		0.519
No	37 (52.1)
Yes	34 (47.9)
Resection of metastatic tumors1		0.002	0.33	0.16-0.67	0.002
No	21 (29.6)
Yes	50 (70.4)

¹Resection of metastasis: Including R0 and R1 resection of all metastatic tumors. ARID1A: AT-rich interactive domain 1A.