Research Report
Copyright ©2014 Baishideng Publishing Group Inc.
World J Gastroenterol. Dec 14, 2014; 20(46): 17407-17415
Published online Dec 14, 2014. doi: 10.3748/wjg.v20.i46.17407
Table 1 Effect of N-nitro-L-arginine methyl ester on liver function parameters in rats with acute hepatic failure
Gr. (n)Bils, mg/dLASTs, IU/LALTs, IU/LAlbumins, g/dLAmmons, μmol/L
1 (8)
Sham0.40 ± 0.26252.5 ± 149.856.2 ± 9.92.9 ± 0.152.8 ± 38.1
2 (8)
Ga1N3.43 ± 1.351624.12 ± 692.922098.6 ± 886.12.6 ± 0.1275.7 ± 73.7
3 (8)
Sham L-NAME0.28 ± 0.17221.37 ± 150.0582.2 ± 16.22.98 ± 0.1126.9 ± 7.8
4 (8)
L-NAME/Ga1N3.27 ± 0.521309.25 ± 349.481343.75 ± 451.922.73 ± 0.14210 ± 48.18
5 (8)
2xL-NAME/Ga1N2.67 ± 0751682.25 ± 433.241590.12 ± 504.262.68 ± 0.15193.87 ± 80.92
6 (8)
Ga1N /L-NAME2.58 ± 0.461424.87 ± 422.991425 ± 475.612.76 ± 0.15206.37 ± 51.11
7 (8)
G Ga1N/2xL-NAME3.1 ± 0.751691.12 ± 370.801707.12 ± 448.872.7 ± 0.17201.5 ± 42.33
Gr. 2 vs Gr. 1P < 0.004P < 0.0001P < 0.001P < 0.001P < 0.005
Gr. 3 vs Gr. 1P < 0.37P < 0.70P < 0.031P < 0.15P < 0.089
Gr. 4 vs Gr. 2P < 0.77P < 0.30P < 0.064P < 0.17P < 0.068
Gr. 5 vs Gr. 2P < 0.21P < 0.85P < 0.064P < 0.46P < 0.067
Gr. 6 vs Gr. 2P < 0.13P < 0.52P < 0.098P < 0.12P < 0.060
Gr. 7 vs Gr. 2P < 0.57P < 0.82P < 0.31P < 0.40P < 0.036
1The P value is significant. Biochemical parameters of the liver were evaluated 48 h after saline or Ga1N injection. Values are mean ± SE. Gr. 1: Sham; Gr. 2: Given 1.1 g/kg Ga1N; Gr. 3: Sham L-NAME, given 100 mg/kg L-NAME; Gr. 4: Given 100 mg/kg L-NAME 48 h and 24 and before Ga1N; Gr. 5: Given 200 mg/kg L-NAME 48 h and 24 h before Ga1N; Gr. 6: Given 100 mg/kg L-NAME 24 and 48 h after Ga1N; Gr. 7: Given 200 mg/kg L-NAME 24 and 48 h after Ga1N. Bils: Serum bilirubin; ASTs: Serum aspartate aminotransferase; ALTs: Serum alanine aminotransferase; Albumins: Serum albumin; Ammons: Serum ammonium.
Table 2 Effect of amino acid L-arginine on liver function parameters in rats with acute hepatic failure
Gr. (n)Bils, mg/dLASTs, IU/LALTs, IU/LAlbumins, mg/dLAmmons,μmol/L
1 (8)
Sham0.40 ± 0.26252.50 ± 149.856.2 ± 9.92.9 ± 0.152.8 ± 38.1
2 (8)
Ga1N3.43 ± 1.351624.12 ± 692.922098.6 ± 886.12.6 ± 0.1275.7 ± 73.7
8 (8)
Sham L-ARG0.38 ± 0.21126.37 ± 33.9553.75 ± 7.372.67 ± 0.0950.7 ± 46.2
9 (8)
L-ARG/Ga1N2.96 ± 0.671484.87 ± 328.751546.37 ± 349.752.75 ± 0.1199.12 ± 42.8
10 (8)
2xL-ARG/Ga1N2.92 ± 0.891611.75 ± 334.581603.75 ± 364.472.73 ± 0.06193.87 ± 50.49
11 (8)
Ga1N/L-ARG2.86 ± 0.941491.87 ± 421.491486.25 ± 450.832.42 ± 0.21195.25 ± 41.69
12 (8)
Ga1N/2xL-ARG2.96 ± 0.981446.25 ± 219.351435.62 ± 281.332.72 ± 0.11180.5 ± 45.80
gr. 2 vs gr. 1P < 0.004P < 0.0001P < 0.001P < 0.001P < 0.005
gr. 8 vs gr. 1P < 0.11P < 0.047P < 0.60P < 0.0007P < 0.340
gr. 9 vs gr. 2P < 0.41P < 0.63P < 0.14P < 0.09P < 0.032
gr. 10 vs gr. 2P < 0.41P < 0.96P < 0.19P < 0.10P < 0.029
gr. 11 vs gr. 2P < 0.37P < 0.67P < 0.12P < 0.06P < 0.024
gr. 12 vs gr. 2P < 0.46P < 0.52P < 0.08P < 0.20P < 0.011
Biochemical parameters of the liver were evaluated 48 h after saline or Ga1N injection. Values are mean ± SE. Gr. 1: Sham; Gr. 2: Given 1.1 g/kg Ga1N; Gr. 8: Sham L-ARG group, given 150 mg/kg L-ARG; Gr. 9: Given 150 mg/kg L-ARG 48 h and 24 h before Ga1N; Gr. 10: Given 300 mg/kg L-ARG 48 h and 24 h before Ga1N; Gr. 11: Given 150 mg/kg L-ARG 24 and 48 h after Ga1N; Gr. 12: Given 300 mg/kg L-ARG 24 and 48 h before Ga1N. Bils: Serum bilirubin; ASTs: Serum aspartate aminotransferase; ALTs: Serum alanine aminotransferase; Albumins: Serum albumin; Ammons: Serum ammonium.