Effect of Danshao Huaxian capsule on Gremlin and bone morphogenetic protein-7 expression in hepatic fibrosis in rats

doi:10.3748/wjg.v20.i40.14875

Advanced Search

BPG is committed to discovery and dissemination of knowledge

Home / Archive / Volume 20, Issue 40

This Article

Academic Content and Language Evaluation of This Article

Citation of this article

Corresponding Author of This Article

Research Domain of This Article

Article-Type of This Article

Open-Access Policy of This Article

Times Cited Counts in Google of This Article

Number of Hits and Downloads for This Article

Total Article Views (7922)

All Articles published online

The chart showing PDF series, WORD series, HTML series, Figures (1-5) series, Tables (1-5) series.

Item

Count

PDF

483

WORD

218

HTML

4324

Figures (1-5)

223

Tables (1-5)

192

Sum=5440

Publishing Process of This Article

The chart showing Browse series, Download series.

Item

Count

Browse

1117

Download

1365

Sum=2482

Oct 28, 2014 (publication date) through Dec 20, 2024

Times Cited of This Article

Times Cited (10)

Journal Information of This Article

Publication Name

World Journal of Gastroenterology

ISSN

1007-9327

Publisher of This Article

Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Original Article

World J Gastroenterol. Oct 28, 2014; 20(40): 14875-14883
Published online Oct 28, 2014. doi: 10.3748/wjg.v20.i40.14875

Table 1 Comparison of serum levels of alanine transaminase and aspartate transaminase, as well as the liver index among groups

Group	n	ALT (U/L)	AST (U/L)	Liver index (g/g)
A	15	37.22 ± 4.53	82.92 ± 2.91	2.65 ± 0.06
B	13	189.09 ± 19.85^b	192.40 ± 16.93^b	4.66 ± 0.20^b
C	15	149.02 ± 25.15^b^d	166.05 ± 12.90^b^d	2.78 ± 0.09^b^d
D	14	103.57 ± 7.4^b^d^f	125.80 ± 9.12^b^d^f	2.74 ± 0.09^d
E	15	92.48 ± 13.27^b^d^f	101.35 ± 9.30^b^d^f	2.71 ± 0.10^d

Data are expressed as mean ± SD. A: Normal group; B: Model group; C: Natural recovery group; D: Low dose Danshao Huaxian capsule (DHC) treated group; E: High dose DHC treated group.

^bP < 0.01 vs group A;

^dP < 0.01 vs group B;

^fP < 0.01 vs group C. ALT: Alanine transaminase; AST: Aspartate transaminase.

Table 2 Comparison of levels of hyaluronic acid, hydroxyproline and transforming growth factor β1 in liver homogenates

Group	n	HA (ng/g)	Hyp (μg/g)	TGF-β1 (μg/g)
A	15	1449.93 ± 121.89	318.20 ± 9.71	284.20 ± 18.32
B	13	8803.30 ± 200.57^b	617.77 ± 8.52^b	736.30 ± 24.40^b
C	15	7558.40 ± 161.88^b^d	530.27 ± 11.88^b^d	651.13 ± 15.75^b^d
D	14	5249.21 ± 111.92^b^d^f	490.00 ± 8.13^b^d^f	523.14 ± 21.29^b^d^f
E	15	4050.73 ± 122.18^b^d^f^h	464.07 ± 13.09^b^d^f^h	441.86 ± 23.18^b^d^f^h

Data are expressed as mean ± SD. A: Normal group; B: Model group; C: Natural recovery group; D: Low dose Danshao Huaxian capsule (DHC) treated group; E: High dose DHC treated group.

^bP < 0.01 vs group A;

^dP < 0.01 vs group B;

^fP < 0.01 vs group C;

^hP < 0.01 vs group D.

Table 3 Grading of hepatic fibrosis degree among groups

Group	n	Hepatic fibrosis degree							Average rank order
Group	n	0	1	2	3	4	5	6	Average rank order
A	15	15	0	0	0	0	0	0	8.00
B	13	0	0	0	0	3	5	5	57.42^a
C	15	0	0	0	2	3	7	3	51.90^a^c
D	14	0	0	3	2	6	3	0	37.64^a^c
E	15	0	0	5	5	4	1	0	30.40^a^c

A: Normal group; B: Model group; C: Natural recovery group; D: Low dose Danshao Huaxian capsule (DHC) treated group; E: High dose DHC treated group.

^aP < 0.05 vs group A;

^cP < 0.05 vs group B.

Table 4 Comparison of the mRNA and protein expression of Gremlin among groups

Group	n	Relative mRNA expression	Positive protein expression rate	Relative protein expression
A	15	49.83 ± 4.20	3.83% ± 0.88%	0.24 ± 0.06
B	13	80.40 ± 5.46^b	38.46% ± 1.70%^b	2.81 ± 0.24^b
C	15	70.37 ± 4.01^b^d	29.50% ± 2.64%^b^d	2.22 ± 0.63^b^d
D	14	64.86 ± 2.83^b^d^f	20.78% ± 1.60%^b^d^f	1.95 ± 0.26^b^d^f
E	15	55.82 ± 5.39^b^d^f^h	17.43% ± 2.02%^b^d^f^g	1.65 ± 0.20^b^d^f^h

Data are expressed as mean ± SD. A: Normal group; B: Model group; C: Natural recovery group; D: Low dose Danshao Huaxian capsule (DHC) treated group; E: High dose DHC treated group.

^bP < 0.01 vs group A;

^dP < 0.01 vs group B;

^fP < 0.01 vs group C; ^gP < 0.05,

^hP < 0.01 vs group D.

Table 5 Comparison of mRNA and protein expression of bone morphogenetic protein-7 among groups

Group	n	Relative mRNA expression	Positive protein expression rate	Relative protein expression
A	15	93.99 ± 7.03	58.29% ± 6.02%	1.05 ± 0.12
B	13	54.00 ± 4.34^b	28.97% ± 3.14%^b	0.48 ± 0.31^b
C	15	62.28 ± 4.51^b^d	35.85% ± 3.50%^b^d	0.60 ± 0.37^b^d
D	14	73.52 ± 4.56^b^d^f	41.44% ± 4.77%^b^d^f	0.71 ± 0.06^b^d^f
E	15	81.78 ± 5.38^b^d^f^h	47.49% ± 4.59%^b^d^f^h	0.81 ± 0.07^b^d^f^h

Data are expressed as mean ± SD. A: Normal group; B: Model group; C: Natural recovery group; D: Low dose Danshao Huaxian capsule (DHC) treated group; E: High dose DHC treated group.

^bP < 0.01 vs group A;

^dP < 0.01 vs group B;

^fP < 0.01 vs group C;

^hP < 0.01 vs group D.

Citation: Zhao XK, Cheng ML, Wu RM, Yao YM, Mu M, Zhu JJ, Zhang BF, Zhou MY. Effect of Danshao Huaxian capsule on Gremlin and bone morphogenetic protein-7 expression in hepatic fibrosis in rats. World J Gastroenterol 2014; 20(40): 14875-14883
URL: https://www.wjgnet.com/1007-9327/full/v20/i40/14875.htm
DOI: https://dx.doi.org/10.3748/wjg.v20.i40.14875