Effect of Danshao Huaxian capsule on Gremlin and bone morphogenetic protein-7 expression in hepatic fibrosis in rats

doi:10.3748/wjg.v20.i40.14875

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Oct 28, 2014 (publication date) through Jun 8, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Oct 28, 2014; 20(40): 14875-14883
Published online Oct 28, 2014. doi: 10.3748/wjg.v20.i40.14875

Table 1 Comparison of serum levels of alanine transaminase and aspartate transaminase, as well as the liver index among groups

Group	n	ALT (U/L)	AST (U/L)	Liver index (g/g)
A	15	37.22 ± 4.53	82.92 ± 2.91	2.65 ± 0.06
B	13	189.09 ± 19.85^b	192.40 ± 16.93^b	4.66 ± 0.20^b
C	15	149.02 ± 25.15^b^d	166.05 ± 12.90^b^d	2.78 ± 0.09^b^d
D	14	103.57 ± 7.4^b^d^f	125.80 ± 9.12^b^d^f	2.74 ± 0.09^d
E	15	92.48 ± 13.27^b^d^f	101.35 ± 9.30^b^d^f	2.71 ± 0.10^d

Data are expressed as mean ± SD. A: Normal group; B: Model group; C: Natural recovery group; D: Low dose Danshao Huaxian capsule (DHC) treated group; E: High dose DHC treated group.

^bP < 0.01 vs group A;

^dP < 0.01 vs group B;

^fP < 0.01 vs group C. ALT: Alanine transaminase; AST: Aspartate transaminase.

Table 2 Comparison of levels of hyaluronic acid, hydroxyproline and transforming growth factor β1 in liver homogenates

Group	n	HA (ng/g)	Hyp (μg/g)	TGF-β1 (μg/g)
A	15	1449.93 ± 121.89	318.20 ± 9.71	284.20 ± 18.32
B	13	8803.30 ± 200.57^b	617.77 ± 8.52^b	736.30 ± 24.40^b
C	15	7558.40 ± 161.88^b^d	530.27 ± 11.88^b^d	651.13 ± 15.75^b^d
D	14	5249.21 ± 111.92^b^d^f	490.00 ± 8.13^b^d^f	523.14 ± 21.29^b^d^f
E	15	4050.73 ± 122.18^b^d^f^h	464.07 ± 13.09^b^d^f^h	441.86 ± 23.18^b^d^f^h

Data are expressed as mean ± SD. A: Normal group; B: Model group; C: Natural recovery group; D: Low dose Danshao Huaxian capsule (DHC) treated group; E: High dose DHC treated group.

^bP < 0.01 vs group A;

^dP < 0.01 vs group B;

^fP < 0.01 vs group C;

^hP < 0.01 vs group D.

Table 3 Grading of hepatic fibrosis degree among groups

Group	n	Hepatic fibrosis degree							Average rank order
Group	n	0	1	2	3	4	5	6	Average rank order
A	15	15	0	0	0	0	0	0	8.00
B	13	0	0	0	0	3	5	5	57.42^a
C	15	0	0	0	2	3	7	3	51.90^a^c
D	14	0	0	3	2	6	3	0	37.64^a^c
E	15	0	0	5	5	4	1	0	30.40^a^c

A: Normal group; B: Model group; C: Natural recovery group; D: Low dose Danshao Huaxian capsule (DHC) treated group; E: High dose DHC treated group.

^aP < 0.05 vs group A;

^cP < 0.05 vs group B.

Table 4 Comparison of the mRNA and protein expression of Gremlin among groups

Group	n	Relative mRNA expression	Positive protein expression rate	Relative protein expression
A	15	49.83 ± 4.20	3.83% ± 0.88%	0.24 ± 0.06
B	13	80.40 ± 5.46^b	38.46% ± 1.70%^b	2.81 ± 0.24^b
C	15	70.37 ± 4.01^b^d	29.50% ± 2.64%^b^d	2.22 ± 0.63^b^d
D	14	64.86 ± 2.83^b^d^f	20.78% ± 1.60%^b^d^f	1.95 ± 0.26^b^d^f
E	15	55.82 ± 5.39^b^d^f^h	17.43% ± 2.02%^b^d^f^g	1.65 ± 0.20^b^d^f^h

Data are expressed as mean ± SD. A: Normal group; B: Model group; C: Natural recovery group; D: Low dose Danshao Huaxian capsule (DHC) treated group; E: High dose DHC treated group.

^bP < 0.01 vs group A;

^dP < 0.01 vs group B;

^fP < 0.01 vs group C; ^gP < 0.05,

^hP < 0.01 vs group D.

Table 5 Comparison of mRNA and protein expression of bone morphogenetic protein-7 among groups

Group	n	Relative mRNA expression	Positive protein expression rate	Relative protein expression
A	15	93.99 ± 7.03	58.29% ± 6.02%	1.05 ± 0.12
B	13	54.00 ± 4.34^b	28.97% ± 3.14%^b	0.48 ± 0.31^b
C	15	62.28 ± 4.51^b^d	35.85% ± 3.50%^b^d	0.60 ± 0.37^b^d
D	14	73.52 ± 4.56^b^d^f	41.44% ± 4.77%^b^d^f	0.71 ± 0.06^b^d^f
E	15	81.78 ± 5.38^b^d^f^h	47.49% ± 4.59%^b^d^f^h	0.81 ± 0.07^b^d^f^h

Data are expressed as mean ± SD. A: Normal group; B: Model group; C: Natural recovery group; D: Low dose Danshao Huaxian capsule (DHC) treated group; E: High dose DHC treated group.

^bP < 0.01 vs group A;

^dP < 0.01 vs group B;

^fP < 0.01 vs group C;

^hP < 0.01 vs group D.

Citation: Zhao XK, Cheng ML, Wu RM, Yao YM, Mu M, Zhu JJ, Zhang BF, Zhou MY. Effect of Danshao Huaxian capsule on Gremlin and bone morphogenetic protein-7 expression in hepatic fibrosis in rats. World J Gastroenterol 2014; 20(40): 14875-14883
URL: https://www.wjgnet.com/1007-9327/full/v20/i40/14875.htm
DOI: https://dx.doi.org/10.3748/wjg.v20.i40.14875