Bortezomib effect on E2F and cyclin family members in human hepatocellular carcinoma cell lines

Table 1 Primer sequences performed quantitative real-time reverse transcription polymerase chain reaction

GenBank Number	Protein	Primer pair	TA(°C)	Amplif.Region	Length (bp)
NM_005225	E2F1	(F) 5’-CCAGGAAAAGGTGTGAAATC-3’ (R) 5’-AAGCGCTTGGTGGTCAGATT-3’	62	466-539	74
NM_004091	E2F2	(F) 5’-CAAGTTGTGCGATGCCTGC-3’ (R) 5’-TCCCAATCCCCTCCAGATC-3’	65	645-714	80
NM_001949	E2F3	(F) 5’-AAGTGCCTGACTCAATAGAGAGCC-3’ (R) 5’-AGTCTCTTCTGGACATAAGTAAACCTCA-3’	62	1307-1392	86
NM_001950	E2F4	(F) 5’-GCAGACCCCACAGGTGTTTT-3’ (R) 5’-GCTCCGAGCTCATGCACTCT-3’	62	1081-1162	82
NM_001951	E2F5	(F) 5’-TTGCTTTAATGGTGATACACTTTTGG-3’ (R) 5’-TCTGACCCATTTCTGGAATGG-3’	62	577-659	83
NM_198256	E2F6	(F) 5’-GAAAATGAAAGACTAGCATATGTGACCT-3’ (R) 5’-CTTTAACTGCAATGACGATCTGTTC-3’	62	818-902	85
NM_203394	E2F7	(F) 5’-AGGGATGGAGGTAAATTGTTTAACACT-3’ (R) 5’-TTTCCCCATCTTCAACTGCAA-3’	65	233-318	86
NM_024680	E2F8	(F) 5’-CTGATCTGCGAACAGGATATTAAAAC-3’ (R) 5’-AAAATGAAAAATCTGGAGTTCCTCC-3’	65	399-492	94
NM_002046	GAPDH	(F) 5’-CCCATCACCATCTTCCAGGAG-3’ (R) 5’-CTTCTCCATGGTGGTGAAGACG-3’	62	319-423	105