Protective effect of bone marrow mesenchymal stem cells in intestinal barrier permeability after heterotopic intestinal transplantation

doi:10.3748/wjg.v20.i23.7442

Advanced Search

BPG is committed to discovery and dissemination of knowledge

Home / Archive / Volume 20, Issue 23

This Article

Academic Content and Language Evaluation of This Article

Citation of this article

Corresponding Author of This Article

Research Domain of This Article

Article-Type of This Article

Open-Access Policy of This Article

Times Cited Counts in Google of This Article

Number of Hits and Downloads for This Article

Total Article Views (5948)

All Articles published online

The chart showing PDF series, WORD series, HTML series, Figures (1-6) series, Tables (1-3) series.

Item

Count

PDF

362

WORD

146

HTML

3386

Figures (1-6)

231

Tables (1-3)

200

Sum=4325

Publishing Process of This Article

The chart showing Browse series, Download series.

Item

Count

Browse

872

Download

750

Sum=1622

Jun 21, 2014 (publication date) through Nov 18, 2024

Times Cited of This Article

Times Cited (14)

Journal Information of This Article

Publication Name

World Journal of Gastroenterology

ISSN

1007-9327

Publisher of This Article

Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Original Article

World J Gastroenterol. Jun 21, 2014; 20(23): 7442-7451
Published online Jun 21, 2014. doi: 10.3748/wjg.v20.i23.7442

Table 1 Sequences of primers for occludin, zona occludens-1, and β-actin

Tested gene product		Primer sequences	Fragment
Occludin	Upstream	5’-CCTGTTTAGTTAGGTGAAG-3’	156 bp
Occludin	Downstream	5’-TTCCTGAGAAGGGTTATG-3’	156 bp
ZO-1	Upstream	5’-GGGGGATTTATAACTTGGG-3’	321 bp
ZO-1	Downstream	5’-CTGGTTGGATGTCTGTGG-3’	321 bp
β-Actin	Upstream	5’-GCGTGACATTAAAGAGAAGCTG-3’	500 bp
β-Actin	Downstream	5’-AGAAGCATTTGCGGTGCAC-3’	500 bp

ZO-1: Zona occludens-1.

Table 2 Grade of intestinal mucosal injury following heterotopic intestinal transplantation

Group	D 1	D 3	D 5	D 7	D 10
A	8.26 ± 0.65	9.37 ± 0.92	10.36 ± 0.78	12.65 ± 0.65	13.14 ± 0.86
B	17.4 ± 1.76^a	30.64 ± 3.62^a	41.6 ± 2.27^a	67.2 ± 2.57^a	81.74 ± 3.52^a
C	12.17 ± 1.17^c	24.00 ± 1.54^c	30.17 ± 0.41^c	41.83 ± 2.93^c	52.33 ± 1.03^c

All values are mean ± SD (n = 6; the summation of six randomly chosen fields from each rat were evaluated and averaged to determine the degree of mucosal injury). A: Non-rejection group; B: Rejection group; C: Bone marrow mesenchymal stem cells therapy group.

^aP < 0.05 vs group A;

^cP < 0.05 vs group B.

Table 3 Levels of graft diamine oxidase and D-lactic acid following heterotopic intestinal transplantation

	Group	D 1	D 3	D 5	D 7	D 10
DAO (U/mL)	A	10.15 ± 1.10	12.86 ± 1.35	15.76 ± 1.33	18.55 ± 1.77	21.83 ± 1.21
	B	22.36 ± 2.82^a	34.74 ± 5.59^a	44.54 ± 2.77^a	51.61 ± 1.70^a	68.02 ± 2.46^a
	C	18.69 ± 2.17^c	29.79 ± 2.49^c	36.15 ± 3.98^c	40.13 ± 1.21^c	59.87 ± 4.34^c
D-LA (g/L)	A	4.12 ± 0.53	6.13 ± 0.57	8.62 ± 1.67	10.78 ± 0.72	11.67 ± 1.56
	B	6.32 ± 0.46^a	8.65 ± 0.62^a	10.46 ± 0.98^a	12.74 ± 0.68^a	17.74 ± 1.75^a
	C	3.08 ± 0.32^c	4.08 ± 0.30^c	5.031 ±0.18^c	7.25 ± 0.51^c	12.81 ± 0.47^c

All values are mean ± SD (n = 6). A: Non-rejection group; B: Rejection group; C: Bone marrow mesenchymal stem cells therapy group.

^aP < 0.05 vs group A;

^cP < 0.05 vs group B. DAO: Diamine oxidase; D-LA: D-lactic acid.

Citation: Zhang W, Shen ZY, Song HL, Yang Y, Wu BJ, Fu NN, Liu T. Protective effect of bone marrow mesenchymal stem cells in intestinal barrier permeability after heterotopic intestinal transplantation. World J Gastroenterol 2014; 20(23): 7442-7451
URL: https://www.wjgnet.com/1007-9327/full/v20/i23/7442.htm
DOI: https://dx.doi.org/10.3748/wjg.v20.i23.7442