Toll-like receptor 4 polymorphisms to determine acute pancreatitis susceptibility and severity: A meta-analysis

Table 1 Studies included in the meta-analysis

SNP	Ref.	Year	Country	Ethnicity	AP			Control			P value for HWE	Source ofcontrols	AP	Control	Assay
					AA	AB	BB	AA	AB	BB
Asp299Gly	Hofner et al[11]	2006	Hungary	Caucasian	84	7	1	64	7	2	0.01	PB	92	73	Taqman
	Gao et al[10]	2007	China	Asian	101	22	0	71	9	0	0.59	PB	123	80	PCR-RFLP
	Zhang et al[9]	2008	China	Asian	238	0	0	121	0	0	1.00	PB	238	121	PCR-RFLP
	Takagi et al[8]	2009	Japan	Asian	202	0	0	286	0	0	1.00	PB	202	286	PCR-RFLP
	Chen et al[12]	2009	China	Asian	64	15	0	47	6	0	0.76	PB	79	53	PCR-RFLP
	Guenther et al[7]	2010	Germany, United States	Caucasian	A 991 G 51			A 725 G 45				PB	521	385	Taqman
Thr399Ile	Hofner et al[11]	2006	Hungary	Caucasian	85	6	1	64	7	2	0.01	PB	92	73	Taqman
	Takagi et al[8]	2009	Japan	Asian	202	0	0	286	0	0	1.00	PB	202	286	PCR-RFLP
	Guenther et al[7]	2010	Germany, United States	Caucasian	C 977 T49			C 728 T 36				PB	521	385	Taqman

SNP: Single nucleotide polymorphisms; AP: Acute pancreatitis; PB: Population-based study; HWE: Hardy-Weinberg equilibrium in controls; PCR-RFLP: Polymerase chain reaction-restriction fragment length polymorphism.

Table 2 Studies of TLR4 Asp299Gly polymorphism and severity of acute pancreatitis

Ref.	Year	Country	Ethnicity	Assay	MAP			SAP
					AA	AG	GG	AA	AG	GG
Hofner et al[11]	2006	Hungary	Caucasian	Taqman	41	1	0	43	6	1
Zhang et al[9]	2008	China	Asian	PCR-RFLP	104	2	0	128	4	0
Chen et al[12]	2009	China	Asian	PCR-RFLP	32	8	0	32	7	0
Guenther et al[7]	2010	Germany, United States	Caucasian	Taqman	A 587 G 33			A 404 G 18

PCR-RFLP: Polymerase chain reaction-restriction fragment length polymorphism; MAP: Mild acute pancreatitis; SAP: Severe acute pancreatitis.

Table 3 Meta-analysis of TLR4 Asp299Gly polymorphism and acute pancreatitis risk

	Test of association			Model	Test ofheterogeneity
	OR	95%CI	P value		P value	I2(%)
Total
A vs G	1.022	0.748-1.397	0.891	F	0.446	0.000
AA vs GG	1.537	0.466-5.075	0.480	F	0.971	0.000
AG vs GG	1.828	0.454-7.368	0.396	F	0.992	0.000
AA vs AG/GG	1.576	0.477-5.205	0.456	F	0.973	0.000
AA/AG vs GG	0.764	0.458-1.277	0.305	F	0.570	0.000
Asian
A vs G	0.628	0.349-1.133	0.122	F	0.874	0.000
AA vs GG	1.273	0.316-5.126	0.734	F	0.965	0.000
AG vs GG	1.760	0.338-9.153	0.502	F	0.966	0.000
AA vs AG/GG	1.326	0.330-5.339	0.691	F	0.961	0.000
AA/AG vs GG	0.606	0.330-1.115	0.107	F	0.861	0.000
Caucasian
A vs G	1.264	0.869-1.839	0.221	F	0.592	0.000
AA vs GG	2.625	0.233-29.591	0.435	F	-	-
AG vs GG	2.000	0.146-27.447	0.604	F	-	-
AA vs AG/GG	2.563	0.228-28.840	0.446	F	-	-
AA/AG vs GG	1.477	0.540-4.039	0.448	F	-	-
PCR-RFLP
A vs G	0.628	0.349-1.133	0.122	F	0.874	0.000
AA vs GG	1.273	0.316-5.126	0.734	F	0.965	0.000
AG vs GG	1.760	0.338-9.153	0.502	F	0.966	0.000
AA vs AG/GG	1.326	0.330-5.339	0.691	F	0.961	0.000
AA/AG vs GG	0.606	0.330-1.115	0.107	F	0.861	0.000
Taqman
A vs G	1.264	0.869-1.839	0.221	F	0.592	0.000
AA vs GG	2.625	0.233-29.591	0.435	F	-	-
AG vs GG	2.000	0.146-27.447	0.604	F	-	-
AA vs AG/GG	2.563	0.228-28.840	0.446	F	-	-
AA/AG vs GG	1.477	0.540-4.039	0.448	F	-	-

Table 4 Meta-analysis of TLR4 Asp299Gly polymorphism and severity of acute pancreatitis (A vs G)

Comparison	Test of association			Model	Test ofheterogeneity		Test ofpublication bias
	OR	95%CI	P value		P value	I2(%)	P value
MAP vs SAP	1.040	0.658-1.643	0.866	F	0.219	32.300	0.124
MAP vs Control	1.112	0.747-1.655	0.601	F	0.159	42.000	0.955
SAP vs Control	1.054	0.691-1.609	0.807	F	0.334	11.800	0.020

Table 5 Meta-analysis of TLR4 Thr399Ile polymorphism and acute pancreatitis risk

Geneticmodels	Test of association			Model	Test of heterogeneity
	OR	95%CI	P value		P value	I2(%)
C vs T	1.090	0.736-1.614	0.667	F	0.503	0.000
CC vs TT	1.523	0.258-9.012	0.643	F	0.481	0.000
CT vs TT	1.455	0.166-12.756	0.735	F	0.823	0.000
CC vs CT/TT	1.494	0.253-8.844	0.658	F	0.493	0.000
CC/CT vs TT	1.530	0.580-4.041	0.390	F	0.560	0.000

F: Fixed-effects model.