Transcription factor ERG is a specific and sensitive diagnostic marker for hepatic angiosarcoma

Table 1 Antibodies used for expression analysis in hepatic angiosarcoma samples

Protein name	Antibody clone	Source	Concentration	Location
Vimentin	V9	Invitrogen	1:200	C
CD31	Jc70A	Gene Tech	1:100	M
CD34	QBEnd-10	Dako	1:50	M
FVIIIRAg	F8/86	Dako	1:50	C
ERG	Ep111	Epitomics	1:100	N
CK	Polyclonal	Dako	1:100	C
GPC-3	1G12	Cell marque	1:150	C;M
Hepatocyte	OCH1E5	Zeta	1:100	C
CD117	Polyclonal	Dako	1:400	M
Desmin	D33	Dako	1:100	C
Ki-67	MIB-1	Dako	1:200	N

C: Cytoplasm; M: Membrane; N: Nucleus; FVIIIRAg: Factor VIII-related antigen; CK: Pan-cytokeratin; GPC-3: Glypican-3; CD117: C-kit proto-oncogene.

Table 2 Clinical and pathological features of the hepatic angiosarcoma cohort

No.	Gender	Age/yr	Clinical symptoms	Tumor location	Maximum tumor size/cm	Treatment methods	Follow-up
11	Male	42	Abdominal discomfort	Left lateral lobe of the liver	5 × 3 × 3	Adjuvant therapy	Spleen metastasis at treatment and died 10 mo later
21	Female	30	Abdominal discomfort	Left inner hepatic lobe	6 × 4 × 3	Adjuvant therapy	Unable to follow-up after 9 mo
3	Male	60	Upper abdominal pain	Right hepatic lobe	16 × 15 × 13	Surgery	Died after 12 mo
41	Female	58	Liver pain	Left lobe of liver	9.5 × 9.4 × 6	Adjuvant therapy	Unable to follow-up after 6 mo
51	Female	52	Back pain	Left and right hepatic lobes	13 × 8 × 6	Adjuvant therapy	Thoracic metastasis at treatment and died 8 mo later
6	Male	7	Abdominal pain, fever	Right hepatic lobe	7 × 6 × 3	Surgery	Died 1 mo later
7	Female	52	Upper abdominal pain	Left hepatic lobe	12.5 × 12 × 4	Surgery	Post-operative thoracic and lung metastases and died 31 mo later
8	Female	62	Abdominal discomfort	Left hepatic lobe	7.5 × 6 × 3	Surgery	Post-operative lung metastases and died 34 mo later
91	Male	68	Upper abdominal pain	Right hepatic lobe	10 × 8 × 6	Adjuvant therapy	Portal vein cancer thrombosis at diagnosis and died 9 mo later
10	Female	29	Hypo-proteinemia	Left and right hepatic lobes	27 × 20 × 9	Liver transplant	Spleen metastasis at diagnosis and died during surgery
11	Male	49	Asymptomatic	Right hepatic lobe	3 × 3 × 2	Surgery	Bone metastases and died 43 mo later
12	Female	62	Upper abdominal pain	Right hepatic lobe	3 × 2 × 2	Liver Transplant	Recurrence of tumor in the transplanted liver and died 4 mo later
13	Female	32	Asymptomatic	Right hepatic lobe	6 × 5 × 3	Surgery	Died 3 mo later
14	Female	51	Upper abdominal pain	Right hepatic lobe	10 × 6 × 5	Surgery	Died 8 mo later
15	Male	53	Upper abdominal pain	Left hepatic lobe	27 × 17 × 6	Surgery	Recurrence after 4 mo and died 7 mo later
16	Male	49	Upper abdominal pain	Right hepatic lobe	11 × 7 × 5	Surgery	Died 10 mo later
171	Male	66	Back pain	Right hepatic lobe	6 × 5 × 4	Adjuvant therapy	Thoracic metastasis at diagnosis and died 5 mo later
18	Male	86	Upper abdominal pain	Left and right hepatic lobes	8 × 7 × 3	Autopsy findings without treatment	Died due to tumor rupture
19	Male	86	Upper abdominal pain	Right hepatic lobe	9 × 9 × 6	Autopsy findings without treatment	Tumor metastasis and died due to heart failure
20	Male	70	Palpitations	Right hepatic lobe	4.5 × 4 × 3	Autopsy findings without treatment	Tumor metastasis and died due to heart failure
211	Female	45	Cough	Right hepatic lobe	4.5 × 4 × 4	Adjuvant therapy	Lung metastasis at diagnosis and died 6 mo later
22	Male	21	Right shoulder pain	Right hepatic lobe	16.5 × 15 × 8	Surgery	Tumor recurrence and died 8 mo later
231	Male	21	Upper abdominal pain	Right hepatic lobe	3.5 × 3 × 3	Adjuvant therapy	Unable to follow-up after 10 mo
24	Male	63	Upper abdominal pain	Right hepatic lobe	3.5 × 3.5 × 3	Surgery	Tumor recurrence after 18 mo and died 21 mo later

¹Patients who received percutaneous biopsies.

Table 3 Means for survival time

Factor 1	Mean1
	Estimate	Std. error	95%CI
			Lower bound	Upper bound
1	12.196	2.788	6.731	17.660

¹Estimation was limited to the largest survival time if it is censored.