Niu TH, Jiang M, Xin YN, Jiang XJ, Lin ZH, Xuan SY. Lack of association between apolipoprotein C3 gene polymorphisms and risk of nonalcoholic fatty liver disease in a Chinese Han population. World J Gastroenterol 2014; 20(13): 3655-3662 [PMID: 24707151 DOI: 10.3748/wjg.v20.i13.3655]
Corresponding Author of This Article
Shi-Ying Xuan, PhD, Professor, Department of Gastroenterology, Qingdao Municipal Hospital, Qingdao 266071, Shandong Province, China. xsy_emuch@126.com
Research Domain of This Article
Gastroenterology & Hepatology
Article-Type of This Article
Research Report
Open-Access Policy of This Article
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Table 2 Distribution of genotype and allele frequencies of apolipoprotein C3 (-455, -482) and patatin-like phospholipase domain-containing protein 3 (rs738409) in nonalcoholic fatty liver disease patients and controls n (%)
NAFLD
Controls
χ2
P value
-455 genotype
TT
102 (26.2)
104 (25.4)
TC
180 (46.2)
195 (47.7)
CC
108 (27.7)
110 (26.9)
0.186
0.911
Allele T
384 (49.2)
403 (49.3)
C
396 (50.8)
415 (51.2)
0.000
1.000
-482 genotype
CC
107 (27.4)
104 (25.4)
CT
176 (45.1)
203 (49.6)
TT
107 (27.4)
102 (51.2)
1.635
0.442
Allele C
390 (50.0)
411 (50.2)
T
390 (50.0)
407 (49.8)
0.010
0.960
rs738409 genotype
CC
48 (12.3)
183 (44.7)
CG
153 (39.2)
176 (43.0)
GG
189 (48.5)
50 (12.2)
160.98
0.000
Allele C
249 (31.9)
542 (66.3)
G
531 (68.1)
276 (33.7)
188.31
0.000
Table 3 Odds ratios for nonalcoholic fatty liver disease according to apolipoprotein C3 and patatin-like phospholipase domain-containing protein 3 genotypes and alleles in the study population
Unadjusted model
Adjusted model
OR (95%CI)
P value
OR (95%CI)
P value
APOC3 gene
-455TT
1
1
-455TC + CC
0.96 (0.70-1.32 )
0.82
1.06 (0.72-1.57 )
0.76
-482CC
1
1
-482CT + TT
0.90 (0.66-1.24 )
0.52
1.00 (0.68-1.48 )
1.00
PNPLA3 gene
CC
1
1
CG + GG
5.77 (4.03-8.27)
0.00
3.70 (2.44-5.60)
0.00
Table 4 Clinical characteristics of apolipoprotein C3 wild-type homozygotes (rs2854117 C/C and rs2854116 T/T) and carriers of one or more variant alleles (rs2854117 T and rs2854116 C) in the study population
Characteristic
Overall series
NAFLD patients
Controls
Noncarriers
Carriers
P value
Noncarriers
Carriers
P value
Noncarriers
Carriers
P value
n
197
602
96
294
101
308
Female/male
110/87
306/296
0.250
48/48
157/137
0.638
62/39
149/159
0.903
Age (yr)
47.95 ± 16.86
48.95 ± 15.76
0.447
49.16 ± 17.23
49.96 ± 15.83
0.672
46.80 ± 16.51
47.98 ± 15.65
0.516
BMI (kg/m2)
23.69 ± 4.08
23.42 ± 3.73
0.387
25.21 ± 2.48
24.70 ± 2.91
0.123
22.14 ± 4.07
22.19 ± 4.10
0.910
Waist (cm)
78.97 ± 9.56
80.17 ± 9.66
0.130
85.36 ± 7.11
83.79 ± 8.49
0.074
72.90 ± 7.40
76.72 ± 9.45
0.000
ALT (U/L)
39.47 ± 34.79
39.46 ± 31.98
0.999
48.60 ± 44.85
43.64 ± 38.51
0.294
30.79 ± 17.50
35.48 ± 23.54
0.066
AST (U/L)
32.71 ± 36.37
33.08 ± 37.72
0.904
36.35 ± 42.00
32.44 ± 37.14
0.386
29.25 ± 29.86
33.70 ± 38.31
0.287
FPG (U/L)
5.46 ± 1.26
5.63 ± 1.49
0.135
5.82 ± 1.46
6.09 ± 1.86
0.201
5.12 ± 0.91
5.20 ± 0.79
0.435
TG (mmol/L)
2.67 ± 1.68
2.54 ± 1.47
0.341
2.88 ± 1.29
2.92 ± 1.48
0.840
2.46 ± 1.97
2.20 ± 1.39
0.134
TC (mmol/L)
4.97 ± 0.98
4.96 ± 0.94
0.887
5.18 ± 0.96
5.17 ± 1.01
0.903
4.77 ± 0.96
4.76 ± 0.81
0.935
HDL (mmol/L)
2.25 ± 2.21
1.99 ± 1.72
0.134
1.51 ± 0.46
1.78 ± 1.10
0.001
2.96 ± 2.89
2.19 ± 2.14
0.015
LDL (mmol/L)
3.84 ± 3.09
3.68 ± 2.66
0.477
4.43 ± 3.71
4.24 ± 3.35
0.659
3.28 ± 2.24
3.14 ± 1.61
0.564
FINS (μU/mL )
5.83 ± 1.20
5.90 ± 1.24
0.545
6.22 ± 1.24
6.28 ± 1.36
0.712
5.46 ± 1.03
5.53 ± 1.00
0.554
HOMA-IR (U)
1.42 ± 0.43
1.48 ± 0.52
0.111
1.62 ± 0.57
1.69 ± 0.61
0.309
1.25 ± 0.34
1.28 ± 0.31
0.376
APOC3 (mg/dL)
13.94 ± 2.01 (n = 34)
14.38 ± 1.92 (n = 146)
0.231
13.60 ± 1.90 (n = 19)
14.37 ± 1.98 (n = 80)
0.231
15.22 ± 1.79 (n = 15)
14.20 ± 1.89 (n = 66)
0.059
Table 5 Clinical characteristics of patatin-like phospholipase domain-containing protein 3 and apolipoprotein C3 (independently of patatin-like phospholipase domain-containing protein 3 I148M) wild-type homozygotes and carriers of one or more variant alleles in the study population
Characteristic
PNPLA3
APOC3 (independently of PNPLA3 I148M)
Noncarriers
Carriers
P value
Noncarriers
Carriers
P value
n
231
568
82
149
Female/male
124/107
292/276
0.560
12/70
109/40
0.000
Age (yr)
46.97 ± 16.42
49.41 ± 15.83
0.052
48.89 ± 16.91
45.92 ± 16.11
0.189
BMI (kg/m2)
22.61 ± 4.08
23.84 ± 3.65
0.000
22.04 ± 4.17
22.92 ± 4.00
0.118
Waist (cm)
75.67 ± 9.00
81.59 ± 9.37
0.000
72.96 ± 7.64
77.16 ± 9.37
0.000
ALT (U/L)
36.03 ± 24.46
40.86 ± 35.40
0.028
34.67 ± 20.30
36.77 ± 26.51
0.501
AST (U/L)
34.07 ± 37.80
32.55 ± 37.21
0.601
32.55 ± 37.21
34.07 ± 37.80
0.662
FPG (U/L)
5.30 ± 1.09
5.71 ± 1.54
0.000
5.26 ± 0.82
5.33 ± 1.21
0.618
TG (mmol/L)
2.34 ± 1.54
2.67 ± 1.51
0.005
2.17 ± 1.09
2.43 ± 1.73
0.216
TC (mmol/L)
4.79 ± 0.90
5.03 ± 0.95
0.001
4.72 ± 0.76
4.83 ± 0.97
0.343
HDL (mmol/L)
2.64 ± 2.54
1.82 ± 1.43
0.000
2.88 ± 2.63
2.50 ± 2.48
0.281
LDL (mmol/L)
3.28 ± 1.98
3.90 ± 3.02
0.001
3.44 ± 1.92
3.19 ± 2.01
0.367
FINS (μU/mL)
5.67 ± 1.13
5.97 ± 1.26
0.001
5.58 ± 1.02
5.72 ± 1.20
0.335
HOMA-IR (U)
1.34 ± 0.38
1.52 ± 0.53
0.000
1.31 ± 0.33
1.35 ± 0.41
0.354
Citation: Niu TH, Jiang M, Xin YN, Jiang XJ, Lin ZH, Xuan SY. Lack of association between apolipoprotein C3 gene polymorphisms and risk of nonalcoholic fatty liver disease in a Chinese Han population. World J Gastroenterol 2014; 20(13): 3655-3662