Lack of association between apolipoprotein C3 gene polymorphisms and risk of nonalcoholic fatty liver disease in a Chinese Han population

doi:10.3748/wjg.v20.i13.3655

Advanced Search

BPG is committed to discovery and dissemination of knowledge

Home / Archive / Volume 20, Issue 13

This Article

Academic Content and Language Evaluation of This Article

Citation of this article

Corresponding Author of This Article

Research Domain of This Article

Article-Type of This Article

Open-Access Policy of This Article

Times Cited Counts in Google of This Article

Number of Hits and Downloads for This Article

Total Article Views (7201)

All Articles published online

The chart showing PDF series, WORD series, HTML series, Figures (1-3) series, Tables (1-5) series.

Item

Count

PDF

406

WORD

312

HTML

3910

Figures (1-3)

348

Tables (1-5)

293

Sum=5269

Publishing Process of This Article

The chart showing Browse series, Download series.

Item

Count

Browse

996

Download

936

Sum=1932

Apr 7, 2014 (publication date) through Apr 12, 2025

Times Cited of This Article

Times Cited (28)

Journal Information of This Article

Publication Name

World Journal of Gastroenterology

ISSN

1007-9327

Publisher of This Article

Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Research Report

World J Gastroenterol. Apr 7, 2014; 20(13): 3655-3662
Published online Apr 7, 2014. doi: 10.3748/wjg.v20.i13.3655

Table 1 Demographic and clinical features of patients with nonalcoholic fatty liver disease and controls n (%)

	NAFLD patients(n = 390)	Controls(n = 409)	P value
BMI (kg/m²)	24.83 ± 2.82	22.18 ± 4.02	0.000^a
Waist (cm)	84.18 ± 8.19	75.78 ± 9.13	0.000^a
Hypertension	89 (22.8)	42 (10.3)	0.000^c
T2DM	87 (22.3)	36 (8.8)	0.000^c
ALT (U/L)	44.86 ± 40.16	34.32 ± 22.27	0.000^a
AST (U/L)	33.40 ± 32.60	32.60 ± 36.42	0.762
GGT (U/L)	43.82 ± 42.17	34.00 ± 26.92	0.000^a
FPG (mmol/L)	6.03 ± 1.77	5.18 ± 0.82	0.357
UA (mmol/L)	326.03 ± 105.24	332.88 ± 104.74	0.000^a
TG (mmol/L)	2.91 ± 1.43	2.26 ± 1.55	0.000^a
TC (mmol/L)	5.17 ± 1.00	4.77 ± 0.85	0.000^a
HDL (mmol/L)	1.71 ± 0.99	2.38 ± 2.36	0.000^a
LDL (mmol/L)	4.28 ± 3.44	3.17 ± 1.78	0.000^a
FINS (μU/mL)	6.27 ± 1.34	5.51 ± 1.01	0.000^a
HOMA-IR (U)	1.67 ± 0.62	1.27 ± 0.32	0.000^a

Data are expressed as mean ± SD,

^aP < 0.05 vs controls, by unpaired Student’s t test;

^cP < 0.05 vs controls, by χ² test. Body mass index (BMI) was calculated as body weight (kg)/height (m²); ALT: Alanine aminotransferase; AST: Aspartate aminotransferase; GGT: γ-glutamyltransferase; FPG: Fasting blood glucose; UA: uric Acid; TC: Total cholesterol; HDL: High-density lipoprotein; LDL: Low-density lipoprotein; FINS: Fasting blood insulin; NAFLD: Nonalcoholic fatty liver disease; TG: Triglyceride; HOMA-IR: Homeostatic model assessment of insulin resistance.

Table 2 Distribution of genotype and allele frequencies of apolipoprotein C3 (-455, -482) and patatin-like phospholipase domain-containing protein 3 (rs738409) in nonalcoholic fatty liver disease patients and controls n (%)

	NAFLD	Controls	χ²	P value
-455 genotype
TT	102 (26.2)	104 (25.4)
TC	180 (46.2)	195 (47.7)
CC	108 (27.7)	110 (26.9)	0.186	0.911
Allele T	384 (49.2)	403 (49.3)
C	396 (50.8)	415 (51.2)	0.000	1.000
-482 genotype
CC	107 (27.4)	104 (25.4)
CT	176 (45.1)	203 (49.6)
TT	107 (27.4)	102 (51.2)	1.635	0.442
Allele C	390 (50.0)	411 (50.2)
T	390 (50.0)	407 (49.8)	0.010	0.960
rs738409 genotype
CC	48 (12.3)	183 (44.7)
CG	153 (39.2)	176 (43.0)
GG	189 (48.5)	50 (12.2)	160.98	0.000
Allele C	249 (31.9)	542 (66.3)
G	531 (68.1)	276 (33.7)	188.31	0.000

NAFLD: Nonalcoholic fatty liver disease.

Table 3 Odds ratios for nonalcoholic fatty liver disease according to apolipoprotein C3 and patatin-like phospholipase domain-containing protein 3 genotypes and alleles in the study population

	Unadjusted model		Adjusted model
	OR (95%CI)	P value	OR (95%CI)	P value
APOC3 gene
-455TT	1		1
-455TC + CC	0.96 (0.70-1.32 )	0.82	1.06 (0.72-1.57 )	0.76
-482CC	1		1
-482CT + TT	0.90 (0.66-1.24 )	0.52	1.00 (0.68-1.48 )	1.00
PNPLA3 gene
CC	1		1
CG + GG	5.77 (4.03-8.27)	0.00	3.70 (2.44-5.60)	0.00

-455C vs T: OR (95%CI) = 1.00 (0.82-1.22): -482C vs T: OR (95%CI) = 0.99 (0.81-1.21); PNPLA3G vs C: OR (95%CI) = 2.00 (1.80-2.23). The multiple logistic regression model was adjusted for age, gender, body mass index, waist, weight and presence of hypertension and T2DM.

Table 4 Clinical characteristics of apolipoprotein C3 wild-type homozygotes (rs2854117 C/C and rs2854116 T/T) and carriers of one or more variant alleles (rs2854117 T and rs2854116 C) in the study population

Characteristic	Overall series			NAFLD patients			Controls
	Noncarriers	Carriers	P value	Noncarriers	Carriers	P value	Noncarriers	Carriers	P value
n	197	602		96	294		101	308
Female/male	110/87	306/296	0.250	48/48	157/137	0.638	62/39	149/159	0.903
Age (yr)	47.95 ± 16.86	48.95 ± 15.76	0.447	49.16 ± 17.23	49.96 ± 15.83	0.672	46.80 ± 16.51	47.98 ± 15.65	0.516
BMI (kg/m²)	23.69 ± 4.08	23.42 ± 3.73	0.387	25.21 ± 2.48	24.70 ± 2.91	0.123	22.14 ± 4.07	22.19 ± 4.10	0.910
Waist (cm)	78.97 ± 9.56	80.17 ± 9.66	0.130	85.36 ± 7.11	83.79 ± 8.49	0.074	72.90 ± 7.40	76.72 ± 9.45	0.000
ALT (U/L)	39.47 ± 34.79	39.46 ± 31.98	0.999	48.60 ± 44.85	43.64 ± 38.51	0.294	30.79 ± 17.50	35.48 ± 23.54	0.066
AST (U/L)	32.71 ± 36.37	33.08 ± 37.72	0.904	36.35 ± 42.00	32.44 ± 37.14	0.386	29.25 ± 29.86	33.70 ± 38.31	0.287
FPG (U/L)	5.46 ± 1.26	5.63 ± 1.49	0.135	5.82 ± 1.46	6.09 ± 1.86	0.201	5.12 ± 0.91	5.20 ± 0.79	0.435
TG (mmol/L)	2.67 ± 1.68	2.54 ± 1.47	0.341	2.88 ± 1.29	2.92 ± 1.48	0.840	2.46 ± 1.97	2.20 ± 1.39	0.134
TC (mmol/L)	4.97 ± 0.98	4.96 ± 0.94	0.887	5.18 ± 0.96	5.17 ± 1.01	0.903	4.77 ± 0.96	4.76 ± 0.81	0.935
HDL (mmol/L)	2.25 ± 2.21	1.99 ± 1.72	0.134	1.51 ± 0.46	1.78 ± 1.10	0.001	2.96 ± 2.89	2.19 ± 2.14	0.015
LDL (mmol/L)	3.84 ± 3.09	3.68 ± 2.66	0.477	4.43 ± 3.71	4.24 ± 3.35	0.659	3.28 ± 2.24	3.14 ± 1.61	0.564
FINS (μU/mL )	5.83 ± 1.20	5.90 ± 1.24	0.545	6.22 ± 1.24	6.28 ± 1.36	0.712	5.46 ± 1.03	5.53 ± 1.00	0.554
HOMA-IR (U)	1.42 ± 0.43	1.48 ± 0.52	0.111	1.62 ± 0.57	1.69 ± 0.61	0.309	1.25 ± 0.34	1.28 ± 0.31	0.376
APOC3 (mg/dL)	13.94 ± 2.01 (n = 34)	14.38 ± 1.92 (n = 146)	0.231	13.60 ± 1.90 (n = 19)	14.37 ± 1.98 (n = 80)	0.231	15.22 ± 1.79 (n = 15)	14.20 ± 1.89 (n = 66)	0.059

Data are shown as mean ± SD. Statistically significant differences were tested using the Student’s unpaired t test or by χ² test when appropriate. A P value < 0.05 was considered significant. ALT: Alanine aminotransferase; AST: Aspartate aminotransferase; GGT: γ-glutamyltransferase; FPG: Fasting blood glucose; UA: uric Acid; TC: Total cholesterol; HDL: High-density lipoprotein; LDL: Low-density lipoprotein; FINS: Fasting blood insulin; NAFLD: Nonalcoholic fatty liver disease; TG: Triglyceride; HOMA-IR: Homeostatic model assessment of insulin resistance; BMI: Body mass index.

Table 5 Clinical characteristics of patatin-like phospholipase domain-containing protein 3 and apolipoprotein C3 (independently of patatin-like phospholipase domain-containing protein 3 I148M) wild-type homozygotes and carriers of one or more variant alleles in the study population

Characteristic	PNPLA3			APOC3 (independently of PNPLA3 I148M)
	Noncarriers	Carriers	P value	Noncarriers	Carriers	P value
n	231	568		82	149
Female/male	124/107	292/276	0.560	12/70	109/40	0.000
Age (yr)	46.97 ± 16.42	49.41 ± 15.83	0.052	48.89 ± 16.91	45.92 ± 16.11	0.189
BMI (kg/m²)	22.61 ± 4.08	23.84 ± 3.65	0.000	22.04 ± 4.17	22.92 ± 4.00	0.118
Waist (cm)	75.67 ± 9.00	81.59 ± 9.37	0.000	72.96 ± 7.64	77.16 ± 9.37	0.000
ALT (U/L)	36.03 ± 24.46	40.86 ± 35.40	0.028	34.67 ± 20.30	36.77 ± 26.51	0.501
AST (U/L)	34.07 ± 37.80	32.55 ± 37.21	0.601	32.55 ± 37.21	34.07 ± 37.80	0.662
FPG (U/L)	5.30 ± 1.09	5.71 ± 1.54	0.000	5.26 ± 0.82	5.33 ± 1.21	0.618
TG (mmol/L)	2.34 ± 1.54	2.67 ± 1.51	0.005	2.17 ± 1.09	2.43 ± 1.73	0.216
TC (mmol/L)	4.79 ± 0.90	5.03 ± 0.95	0.001	4.72 ± 0.76	4.83 ± 0.97	0.343
HDL (mmol/L)	2.64 ± 2.54	1.82 ± 1.43	0.000	2.88 ± 2.63	2.50 ± 2.48	0.281
LDL (mmol/L)	3.28 ± 1.98	3.90 ± 3.02	0.001	3.44 ± 1.92	3.19 ± 2.01	0.367
FINS (μU/mL)	5.67 ± 1.13	5.97 ± 1.26	0.001	5.58 ± 1.02	5.72 ± 1.20	0.335
HOMA-IR (U)	1.34 ± 0.38	1.52 ± 0.53	0.000	1.31 ± 0.33	1.35 ± 0.41	0.354

ALT: Alanine aminotransferase; AST: Aspartate aminotransferase; GGT: γ-glutamyltransferase; FPG: Fasting blood glucose; UA: uric Acid; TC: Total cholesterol; HDL: High-density lipoprotein; LDL: Low-density lipoprotein; FINS: Fasting blood insulin; NAFLD: Nonalcoholic fatty liver disease; TG: Triglyceride; HOMA-IR: Homeostatic model assessment of insulin resistance; PNPLA3: Patatin-like phospholipase domain-containing protein 3; APOC3: Apolipoprotein C3.

Citation: Niu TH, Jiang M, Xin YN, Jiang XJ, Lin ZH, Xuan SY. Lack of association between apolipoprotein C3 gene polymorphisms and risk of nonalcoholic fatty liver disease in a Chinese Han population. World J Gastroenterol 2014; 20(13): 3655-3662
URL: https://www.wjgnet.com/1007-9327/full/v20/i13/3655.htm
DOI: https://dx.doi.org/10.3748/wjg.v20.i13.3655