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©2014 Baishideng Publishing Group Co.
World J Gastroenterol. Mar 14, 2014; 20(10): 2688-2694
Published online Mar 14, 2014. doi: 10.3748/wjg.v20.i10.2688
Published online Mar 14, 2014. doi: 10.3748/wjg.v20.i10.2688
Table 1 Primers and probes used for genotyping CD14-260C/T and -651C/T
Polymorphism | Primers and probes | Length, bp |
-260 C/T | 5'-CACCCACCAGAGAAGGCTTA-3' | 212 |
5'-ATCACCTCCCCACCTCTCTT-3' | ||
Common probe | 5'-CCCCCTCCCTGAAACATCCTTTTTTTTTTTTTTTTTTT-FAM-3' | |
Discriminating | 260C/T-R_G | 77 |
probes | 5'-TTTTTTTTTTTTTTTTTGCAGAATCCTTCCTGTTACGGC | |
260C/T-R_A-3' | 79 | |
5'-TTTTTTTTTTTTTTTTTTTGCAGAATCCTTCCTGTTACGGT-3' | ||
-651C/T | 5'-GGGTAGAATTAGGTTCAAG-3' | 103 |
5'-CTTAATCAAAGGAGCAAGG-3' | 1 | |
Common probe | 5'-GTCTAAAGAAAAATTCCCCCTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT-FAM-3' | |
Discriminating probes | 651C/T_C | 150 |
5'-TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGGTTCAAGAAAAGGAAGTTG-3' | ||
651C/T_T | 152 | |
5'-TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGGTTCAAGAAAAGGAAGTTA-3' |
Table 2 Characteristics of study subjects n (%)
Variable | Controls | GC patients | aP value |
n = 237 | n = 225 | ||
Age, yr | 54.8 ± 11.2 | 54.0 ± 12.3 | NS |
Male | 175 (73.8) | 172 (76.4) | NS |
Clinical stage | |||
I | 7 (3.1) | ||
II | 21 (9.3) | ||
III | 126 (56.0) | ||
IV | 71 (31.6) |
Table 3 Genotype and allele frequencies ofCD14-260C/T and -651C/T in gastric cancer patients and healthy control subjects n (%)
Polymorphism | Case | Control | OR | 95%CI | P value |
n = 225 | n = 237 | ||||
-260 C/T | |||||
Genotypes | |||||
CC | 43 (19.1) | 79 (33.3) | 1.000 | (reference) | - |
CT | 87 (38.7) | 77 (32.5) | 2.076 | 1.282-3.360 | 0.003 |
TT | 95 (42.2) | 81 (34.2) | 2.155 | 1.340-3.466 | 0.001 |
Alleles | |||||
C | 173 (38.4) | 235 (49.6) | 1.000 | (reference) | - |
T | 277 (61.6) | 239 (50.4) | 1.574 | 1.121-2.045 | 0.001 |
-651 C/T | |||||
Genotypes | |||||
CC | 145 (64.4) | 134 (56.5) | 1.000 | (reference) | - |
CT | 66 (29.3) | 83 (35.0) | 0.735 | 0.493-1.096 | 0.130 |
TT | 14 (6.2) | 20 (8.4) | 0.647 | 0.314-1.332 | 0.234 |
Alleles | |||||
C | 356 (79.1) | 351 (74.1) | 1.000 | (reference) | - |
T | 94 (20.9) | 123 (25.9) | 0.753 | 0.55-1.024 | 0.07 |
Table 4 Haplotype distribution of CD14-260C/T and -651 C/T in gastric cancer patients and healthy control subjects n (%)
Haplotypes -260C/T - -651 C/T | Case | Control | OR (95%CI) | P value |
T-C | 244 (57.9) | 206 (46.5) | 1.58 (1.21-2.07) | 0.001 |
C-C | 87 (20.8) | 120 (27.2) | 0.70 (0.51-0.96) | 0.028 |
C-T | 76 (18.1) | 97 (21.9) | 0.79 (0.56-1.10) | 0.157 |
T-T | 13 (3.3) | 19 (4.4) | 0.73 (0.36-1.47) | 0.371 |
- Citation: Li K, Dan Z, Hu XJ, Gesang LB, Ze YG, Bianba ZX, Ciren CM, Nie YQ. Association of CD14/-260 polymorphism with gastric cancer risk in Highland Tibetans. World J Gastroenterol 2014; 20(10): 2688-2694
- URL: https://www.wjgnet.com/1007-9327/full/v20/i10/2688.htm
- DOI: https://dx.doi.org/10.3748/wjg.v20.i10.2688