Susceptibility to ulcerative colitis in Hungarian patients determined by gene-gene interactions

doi:10.3748/wjg.v20.i1.219

Advanced Search

BPG is committed to discovery and dissemination of knowledge

Home / Archive / Volume 20, Issue 1

This Article

Academic Content and Language Evaluation of This Article

Citation of this article

Corresponding Author of This Article

Research Domain of This Article

Article-Type of This Article

Open-Access Policy of This Article

Times Cited Counts in Google of This Article

Number of Hits and Downloads for This Article

Total Article Views (5567)

All Articles published online

The chart showing PDF series, WORD series, HTML series, Tables (1-4) series.

Item

Count

PDF

396

WORD

256

HTML

3324

Tables (1-4)

244

Sum=4220

Publishing Process of This Article

The chart showing Browse series, Download series.

Item

Count

Browse

672

Download

675

Sum=1347

Jan 7, 2014 (publication date) through Feb 7, 2025

Times Cited of This Article

Times Cited (9)

Journal Information of This Article

Publication Name

World Journal of Gastroenterology

ISSN

1007-9327

Publisher of This Article

Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Brief Article

World J Gastroenterol. Jan 7, 2014; 20(1): 219-227
Published online Jan 7, 2014. doi: 10.3748/wjg.v20.i1.219

Table 1 Primer sequences for the analysed variants

Gene	SNP	Primers (5’-3’)
IL23R	rs1004819	Forward: GCATTCTAGGACCGTTTTGG
IL23R	rs1004819	Reverse: ATCTGGTGGAAATATGTGAAACCTA
IL23R	rs2201841	Forward: GGCAAAAGGGAATTGAGAGG
IL23R	rs2201841	Reverse: GGCCTATGATTATGCTTTTTCCTG¹
SLC22A4	rs1050152	Forward: AGAGAGTCCTCCTATCTGATTG
SLC22A4	rs1050152	Reverse: TCCTAGCTATTCTTCCATGC
SLC22A5	rs2631367	Forward: GCCGCTCTGCCTGCCAGC
SLC22A5	rs2631367	Reverse: GGTCGCTATCAGGAACACGGAGGA
IGR2230a_1	rs17622208	Forward: CAGAAGAATGCCCTTGATGTG
IGR2230a_1	rs17622208	Reverse: TCAGAAGCTGTCCATCCCAC
IGR2198a_1	rs11739135	Forward: AGACACTGGGACATCATCTGTCTG
IGR2198a_1	rs11739135	Reverse: GGGCAATTCTATGAGGACATTTAGA
IGR2096a_1	rs12521868	Forward: CAAGATTTCTGCCATAGCCTCCT
IGR2096a_1	rs12521868	Reverse: GGAGGGTGGTGTAGCCAGAGTAG

¹Mismatch base is underlined.

Table 2 Case-control genotypes and allele frequencies of variants in IL23R and inflammatory bowel disease-5 locus n (%)

	UC (n = 320)	Controls (n = 316)	OR (95%CI)¹	P value
IL23R (rs1004819)
GG	126 (39.4)	158 (50.0)
GA	168 (52.5)	134 (42.4)
GA + AA	194 (60.6)	158 (50.0)	1.606 (1.160-2.223)^a	0.004^a
AA	26 (8.1)	24 (7.6)	1.254 (0.696-2.261)	0.452
RAF	0.343	0.287		0.032^a
IL23R (rs2201841)
TT	140 (43.8)	155 (49.1)
TC	150 (46.9)	143 (45.3)
TC + CC	180 (56.3)	161 (51.0)	1.268 (0.920-1.749)	0.147
CC	30 (9.4)	18 (5.7)	1.983 (1.069-3.678)^a	0.030^a
RAF	0.328	0.283		0.242
SLC22A4 (rs1050152)
CC	93 (29.1)	110 (34.8)
CT	159 (49.7)	148 (46.8)
CT + TT	227 (71.0)	206 (65.2)	1.319 (0.935-1.86)	0.115
TT	68 (21.3)	58 (18.4)	1.150 (0.768-1.723)	0.498
RAF	0.46	0.417		0.120
SLC22A5 (rs2631367)
GG	83 (25.9)	89 (28.2)
GC	163 (50.9)	156 (49.4)
GC + CC	237 (74.0)	227 (71.9)	1.138 (0.794-1.631)	0.481
CC	74 (23.1)	71 (22.5)	0.982 (0.669-1.440)	0.925
RAF	0.485	0.471		0.607
IGR2230a_1 (rs17622208)
GG	87 (27.2)	90 (28.5)
AG	160 (50.0)	157 (49.7)
AG + AA	233 (72.8)	226 (71.5)	1.073 (0.751-1.532)	0.698
AA	73 (22.8)	69 (21.8)	0.990 (0.673-1.457)	0.960
RAF	0.478	0.466		0.685
IGR2198a_1 (rs11739135)
GG	105 (32.8)	117 (37.0)
GC	159 (49.7)	150 (47.5)
GC + CC	215 (67.2)	199 (63.0)	1.260 (0.900-1.763)	0.179
CC	56 (17.5)	49 (15.5)	1.169 (0.760-1.797)	0.477
RAF	0.423	0.392		0.260
IGR2096a_1 (rs12521868)
GG	101 (31.6)	117 (37.0)
GT	164 (51.3)	147 (46.5)
GT + TT	219 (68.5)	199 (63.0)	1.256 (0.897-1.760)	0.185
TT	55 (17.2)	52 (16.5)	1.045 (0.680-1.608)	0.840
RAF	0.428	0.397		0.262

¹Adjusted for age and gender.

^aAssociations significant at P < 0.05 vs controls. RAF: Risk allele frequency; UC: Ulcerative colitis.

Table 3 Pairwise analysis of interactions of IBD5 and IL23R to risk of ulcerative colitis

Model	OR (95%CI)	P value
IL23R rs1004819 * IGR2230a_1	1.266 (0.627-2.553)	0.51
IL23R rs1004819 * IGR2198a_1	1.383 (0.711-2.690)	0.340
IL23R rs1004819 * IGR2096a_1	0.942 (0.625-1.420)	0.776
IL23R rs1004819 * SLC22A4	1.017 (0.516-2.002)	0.962
IL23R rs1004819 * SLC22A5	1.116 (0.549-2.270)	0.761
IL23R rs2201841 Ho* IGR2230a_1	0.316 (0.080-1.247)	0.100
IL23R rs2201841 Ho* IGR2198a_1	0.388 (0.105-1.430)	0.155
IL23R rs2201841 Ho* IGR2096a_1	0.413 (0.117-1.458)	0.169
IL23R rs2201841 Ho* SLC22A4	0.118 (0.095-1.301)	0.352
IL23R rs2201841 Ho* SLC22A5	0.298 (0.075-1.179)	0.084

Ho: Homozygotes.

Table 4 Genotype-specific ulcerative colitis odds ratios (with 95%CI) for combinations of variants in IL23R and IBD5

	SLC22A4		SLC22A5		IGR2230a_1		IGR2198a_1		IGR2096a_1
	CC	CT + TT	GG	GC + CC	GG	AG + AA	GG	GC + CC	GG	GT + TT
IL23R rs1004819
GG	1	1.298	1	1.064	1	0.941	1	1.033	1	1.104
		(0.782-2.156)		(0.623-1.815)		(0.556-1.593)		(0.623-1.711)		(0.666-1.831)
		P = 0.313		P = 0.821		P = 0.822		P = 0.900		P = 0.702
GA + AA	1.527	¹2.015	1.424	¹1.691	1.300	1.549	1.263	¹1.803	1.281	¹1.911
	(0.872-2.673)	(1.230-3.300)	(0.776-2.614)	(1.003-2.821)	(0.716-2.359)	(0.927-2.588)	(0.736-2.166)	(1.096-2.966)	(0.744-2.206)	(1.162-3.143)
	P = 0.138	P = 0.005	P = 0.253	P = 0.048	P = 0.388	P = 0.093	P = 0.396	P = 0.020	P = 0.372	P = 0.010
IL23R rs2201841
TT+TC	1	1.328	1	1.254	1	1.184	1	1.296	1	1.385
		(0.989-1.927)		(0.868-1.812		(0.823-1.704)		(0.922-1.822)		(0.982-1.953)
		P = 0.058		P = 0.228		P = 0.363		P = 0.136		P = 0.063
CC	¹3.413	1.716	¹3.946	1.474	¹3.777	1.411	¹3.165	1.592	¹2.977	¹1.701
	(1.169-9.965)	(0.788-3.739	(1.232-12.645)	(0.679-3.200)	(1.181-12.084)	(0.652-3.056)	(1.088-9.206)	(0.735-3.449)	(1.099-8.066)	(0.765-3.784)
	P = 0.018	P = 0.171	P = 0.014	P = 0.324	P = 0.018	P = 0.381	P = 0.027	P = 0.236	P = 0.026	P = 0.189

¹Associations significant at P < 0.05 vs controls.

Citation: Sarlos P, Varszegi D, Csongei V, Magyari L, Jaromi L, Nagy L, Melegh B. Susceptibility to ulcerative colitis in Hungarian patients determined by gene-gene interactions. World J Gastroenterol 2014; 20(1): 219-227
URL: https://www.wjgnet.com/1007-9327/full/v20/i1/219.htm
DOI: https://dx.doi.org/10.3748/wjg.v20.i1.219