Overexpression of kallikrein gene 10 is a biomarker for predicting poor prognosis in gastric cancer

Table 1 Data of the study population

Variable	No. of patients	mean ± SE	Range
Age (yr)	80	51 ± 0.81	35-74
Lymph nodes1	80	30 ± 3.11	0-63
Follow-up (mo)2	80	31 ± 1.98	7-52

¹Number of lymph nodes removed during surgery;

²Follow-up time after surgery.

Table 2 Associations between kallikrein gene 10 status and other variables in 80 patients with gastric cancer

Variable	Total	KLK10-negative	KLK10-positive	P value1
Sex
Male	56	14	42
Female	24	9	15	0.258
Age (yr)
< 60	31	10	21
≥ 60	49	13	36	0.581
Depth of invasion2
T1	27	12	15
T2-T3	23	7	16
T4	30	4	26	0.034
Lymph node metastasis2
N1	21	4	17
N2	35	15	20
N3	24	4	20	0.048
Differentiation2
Well	18	10	8
Moderate	37	7	30
Poor	25	6	19	0.015

¹χ² test;

²TNM stage system of American Joint Committee on Cancer of 2012. KLK10: Kallikrein gene 10.

Table 3 Univariate and multivariate analysis of disease-free survival and overall survival

Survival variable	Disease-free survival			Overall survival
	95%CI2	P value	HR1	95%CI2	P value	HR1
Univariate analysis
KLK10
Negative			1.00			1.00
Positive	0.25-0.74	0.002	0.46	0.26-0.79	0.005	0.43
Age	0.97-1.01	0.46	0.99	0.97-1.01	0.49	0.99
Sex	0.55-1.53	0.75	0.90	0.54-1.51	0.69	0.92
Depth of invasion	1.55-2.81	< 0.001	2.13	1.57-2.89	< 0.001	2.08
Lymph node metastasis	0.97-1.97	0.07	1.42	0.99-2.02	0.051	1.38
Histology	6.42-22.46	< 0.001	10.25	5.73-18.32	< 0.001	12.01
Multivariate analysis
KLK10
Negative		1.00			1.00
Positive	0.31-0.96	0.03	0.58	0.33-1.02	0.06	0.55
Age	0.95-1.00	0.02	0.97	0.95-0.99	0.04	0.97
Sex	0.77-2.22	0.31	1.33	0.77-2.28	0.30	1.31
Depth of invasion	0.99-1.90	0.06	1.42	1.02-1.98	0.03	1.37
Lymph node metastasis	0.84-1.59	0.35	1.21	0.87-1.67	0.24	1.16
Histology	6.55-25.41	0.03	11.11	5.87-21.02	< 0.001	12.90

¹Hazard ratio (HR) estimated from Cox proportional hazard regression model;

²Confidence interval of the estimated HR. KLK10: Kallikrein gene 10.