Original Article
Copyright ©2013 Baishideng Publishing Group Co.
World J Gastroenterol. Dec 28, 2013; 19(48): 9294-9306
Published online Dec 28, 2013. doi: 10.3748/wjg.v19.i48.9294
Table 1 Demographic, clinical and virological characteristics of hepatitis B surface antigen-positive patients with different disease profiles
CharacteristicGroup I HCC (n = 44)Group II CR (n = 22)Group III CH (n = 25)Total (n = 91)
Demographic and clinical dataGender (M/F)33/1118/418/769/22
Age (yr) (mean ± SD)  48.70 ± 10.94a40.68 ± 11.5226.28 ± 11.1040.87 ± 14.66
ALT, IU/L73.50 ± 11.6570.50 ± 41.0956.32 ± 30.0167.45 ± 37.74
HBeAg positive1     7 (24.14)4 (14.3)9 (37.5)20 (26.67)
Virological characteristicsHBV DNA log10 copies/mL14.79 ± 1.41a3.38 ± 1.693.27 ± 2.124.03 ± 1.83
Number genotyped by direct sequencing/RFLP21/199/1219/686
Genotype A33 (82.5)a14 (66.6)15 (60)62 (72.1)
Subgenotype A1 30141559 (95)
Subgenotype A23--3 (5)
Genotype C-1 (4.8)-1 (1.2)
Genotype D   7 (17.5)6 (28.6)10 (40)23 (26.7)
Subgenotype D12-13
Subgenotype D2426
Subgenotype D31--
Table 2 Multiple logistic regression analysis of basal core promoter/precore region in different disease groups
HCC
Non HCC (CR/CH)
OR (95%CI)P value
Gen A (n = 20)Gen D (n = 6)Gen A (n = 26)Gen D (n = 11)
Basic core promoter/ pre core regionA1762T/G1764A + G1764A only16 (80)5 (83)14 (54)3 (27)20.2 (6.3-65)a0.008aNSd
C1766T/T1768A9 (45)1 (5)5 (25)025 (7.3-86)a 14.3 (1.7–119)d0.03a0.05d
1773T (genotype A)19 (95)-25-
1773C (genotype D)-4-7-NSa
G1862T18 (90)2 (33)21 (81)1(9)30.33 (5.62-192.6)dNSa 0.0001d
G1896A04 (67)02 (18)-NSa 0.0002c
A1762T/G1764A + G1862T13 (65)1 (2)13 (50)04.81 (0.6-39.4)bNSa 0.0004b
Complete S regionPre-S deletions5 (33.3)e03 (17.6)1 (9)1.89 (0.54-6.60)bNSbNSd
Table 3 Summary of the pre-S mutations prevalent among the three clinical groups and genotypes
IsolateAge/sexClinicalstatusSubgenotypePreS1
PreS2
Functionsaffected
Start codonNucleotide from the EcoRI start
Amino acids from preS2 start codon
Start codonDeletion sizePositionDeletion sizePosition
CHB4240/MCHA1ATGACG----A
HCC1260/MHCCA1ATGACG----A
HCC2550/MHCCA1ATGACG----A
CHB13716/FCHA1DeletionATG182854-287161-6I
CHB17029/FCHD2ATGATG2135-55716-22T, B, P
CHB20233/MCHA1ATGATG2428-51813-21T, B, P
CHB41335/MCHA1ATGATG649-54221-22B, P
HCC2950/FHCCA1ATGATA3322-541111-22A, T, B, P
HCC3032/MHCCA1ATGATG2430-53813-21T, B, P
HCC3730/FHCCA1ATGATG3324-561111-22T, B, P
HCC3955/MHCCA1ATGATG3324-561111-22T, B, P
HCCT338/MHCCA1ATGATA541-54184-22A, T, B, P, M