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©2013 Baishideng Publishing Group Co.
World J Gastroenterol. Dec 28, 2013; 19(48): 9294-9306
Published online Dec 28, 2013. doi: 10.3748/wjg.v19.i48.9294
Published online Dec 28, 2013. doi: 10.3748/wjg.v19.i48.9294
Table 1 Demographic, clinical and virological characteristics of hepatitis B surface antigen-positive patients with different disease profiles
Characteristic | Group I HCC (n = 44) | Group II CR (n = 22) | Group III CH (n = 25) | Total (n = 91) | |
Demographic and clinical data | Gender (M/F) | 33/11 | 18/4 | 18/7 | 69/22 |
Age (yr) (mean ± SD) | 48.70 ± 10.94a | 40.68 ± 11.52 | 26.28 ± 11.10 | 40.87 ± 14.66 | |
ALT, IU/L | 73.50 ± 11.65 | 70.50 ± 41.09 | 56.32 ± 30.01 | 67.45 ± 37.74 | |
HBeAg positive1 | 7 (24.14) | 4 (14.3) | 9 (37.5) | 20 (26.67) | |
Virological characteristics | HBV DNA log10 copies/mL1 | 4.79 ± 1.41a | 3.38 ± 1.69 | 3.27 ± 2.12 | 4.03 ± 1.83 |
Number genotyped by direct sequencing/RFLP | 21/19 | 9/12 | 19/6 | 86 | |
Genotype A | 33 (82.5)a | 14 (66.6) | 15 (60) | 62 (72.1) | |
Subgenotype A1 | 30 | 14 | 15 | 59 (95) | |
Subgenotype A2 | 3 | - | - | 3 (5) | |
Genotype C | - | 1 (4.8) | - | 1 (1.2) | |
Genotype D | 7 (17.5) | 6 (28.6) | 10 (40) | 23 (26.7) | |
Subgenotype D12 | - | 1 | 3 | ||
Subgenotype D2 | 4 | 2 | 6 | ||
Subgenotype D3 | 1 | - | - |
Table 2 Multiple logistic regression analysis of basal core promoter/precore region in different disease groups
HCC | Non HCC (CR/CH) | OR (95%CI) | P value | ||||
Gen A (n = 20) | Gen D (n = 6) | Gen A (n = 26) | Gen D (n = 11) | ||||
Basic core promoter/ pre core region | A1762T/G1764A + G1764A only | 16 (80) | 5 (83) | 14 (54) | 3 (27) | 20.2 (6.3-65)a | 0.008aNSd |
C1766T/T1768A | 9 (45) | 1 (5) | 5 (25) | 0 | 25 (7.3-86)a 14.3 (1.7–119)d | 0.03a0.05d | |
1773T (genotype A) | 19 (95) | - | 25 | - | |||
1773C (genotype D) | - | 4 | - | 7 | - | NSa | |
G1862T | 18 (90) | 2 (33) | 21 (81) | 1(9) | 30.33 (5.62-192.6)d | NSa 0.0001d | |
G1896A | 0 | 4 (67) | 0 | 2 (18) | - | NSa 0.0002c | |
A1762T/G1764A + G1862T | 13 (65) | 1 (2) | 13 (50) | 0 | 4.81 (0.6-39.4)b | NSa 0.0004b | |
Complete S region | Pre-S deletions | 5 (33.3)e | 0 | 3 (17.6) | 1 (9) | 1.89 (0.54-6.60)b | NSbNSd |
Table 3 Summary of the pre-S mutations prevalent among the three clinical groups and genotypes
Isolate | Age/sex | Clinicalstatus | Subgenotype | PreS1 | PreS2 | Functionsaffected | ||||
Start codon | Nucleotide from the EcoRI start | Amino acids from preS2 start codon | ||||||||
Start codon | Deletion size | Position | Deletion size | Position | ||||||
CHB42 | 40/M | CH | A1 | ATG | ACG | - | - | - | - | A |
HCC12 | 60/M | HCC | A1 | ATG | ACG | - | - | - | - | A |
HCC25 | 50/M | HCC | A1 | ATG | ACG | - | - | - | - | A |
CHB137 | 16/F | CH | A1 | Deletion | ATG | 18 | 2854-2871 | 6 | 1-6 | I |
CHB170 | 29/F | CH | D2 | ATG | ATG | 21 | 35-55 | 7 | 16-22 | T, B, P |
CHB202 | 33/M | CH | A1 | ATG | ATG | 24 | 28-51 | 8 | 13-21 | T, B, P |
CHB413 | 35/M | CH | A1 | ATG | ATG | 6 | 49-54 | 2 | 21-22 | B, P |
HCC29 | 50/F | HCC | A1 | ATG | ATA | 33 | 22-54 | 11 | 11-22 | A, T, B, P |
HCC30 | 32/M | HCC | A1 | ATG | ATG | 24 | 30-53 | 8 | 13-21 | T, B, P |
HCC37 | 30/F | HCC | A1 | ATG | ATG | 33 | 24-56 | 11 | 11-22 | T, B, P |
HCC39 | 55/M | HCC | A1 | ATG | ATG | 33 | 24-56 | 11 | 11-22 | T, B, P |
HCCT3 | 38/M | HCC | A1 | ATG | ATA | 54 | 1-54 | 18 | 4-22 | A, T, B, P, M |
- Citation: Gopalakrishnan D, Keyter M, Shenoy KT, Leena KB, Thayumanavan L, Thomas V, Vinayakumar K, Panackel C, Korah AT, Nair R, Kramvis A. Hepatitis B virus subgenotype A1 predominates in liver disease patients from Kerala, India. World J Gastroenterol 2013; 19(48): 9294-9306
- URL: https://www.wjgnet.com/1007-9327/full/v19/i48/9294.htm
- DOI: https://dx.doi.org/10.3748/wjg.v19.i48.9294