Amelioration of carbon tetrachloride-induced cirrhosis and portal hypertension in rat using adenoviral gene transfer of Akt

Table 1 Portal vein diameter, peak velocity of blood flow, congestion index and blood flow in each experimental group

Group	n	D (mm)	V (cm/s)	CI	Q (mL/min)
Normal	5	1.13 ± 0.24	12.67 ± 0.64	0.0010 ± 0.0003	4.56 ± 1.86
Cirrhosis	20
Untreated	5	1.81 ± 0.19a	10.13 ± 0.68a	0.0021 ± 0.0007a	9.69 ± 2.58a
Saline	5	1.83 ± 0.29a	10.06 ± 0.72a	0.0024 ± 0.0008a	9.32 ± 2.83a
EGFP	5	1.82 ± 0.27a	9.98 ± 0.77a	0.0020 ± 0.0006a	8.97 ± 2.45a
Akt	5	1.28 ± 0.32ceg	11.39 ± 0.63ceg	0.0013 ± 0.0004ceg	5.11 ± 2.30ceg

^aP < 0.05 (vs Normal control);

^cP < 0.05 (vs Untreated group);

^eP < 0.05 (vs Saline group);

^gP < 0.05 [vs enhanced green fluorescent protein (EGFP) group]. No difference was detected among Untreated, Saline and EGFP groups. D: Diameter; V: Peak velocity of blood flow; CI: Congestion index; Q: Blood flow.

Table 2 Liver function parameter in each experiment group in each experimental group

Group	n	ALT (U/L)	AST (U/L)	ALB (g/L)	Hyp (μg/g)
Normal	5	23.5 ± 6.3	109.3 ± 6.1	33.1 ± 2.6	180.5 ± 12.5
Cirrhosis	20
Untreated	5	277.6 ± 25.8a	380.5 ± 16.9a	22.7 ± 3.5a	375.2 ± 17.3a
Saline	5	290.7 ± 22.9a	368.9 ± 23.8a	24.7 ± 3.7a	393.8 ± 22.3a
EGFP	5	285.9 ± 27.3a	374.4 ± 26.7a	23.9 ± 2.9a	388.5 ± 9.8a
Akt	5	126.4 ± 5.8aceg	202.5 ± 9.5aceg	30.7 ± 4.8aceg	245.9 ± 15.6aceg

^aP < 0.05 (vs Normal control);

^cP < 0.05 (vs Untreated group);

^eP < 0.05 (vs Saline group);

^gP < 0.05 [vs enhanced green fluorescent protein (EGFP) group]. No difference was detected among Untreated, Saline and EGFP groups. ALT: Alanine aminotransferase; AST: Aspartate aminotransferase; ALB: Albumin; Hyp: Hepatic hydroxyproline.

Table 3 Portal vein pressure, mean arterial pressure and heart rate in each experiment group at the end of the treatment

Group	n	PVP (mmHg)	MAP (mmHg)	HR
Normal	5	8.96 ± 1.46	83.5 ± 9.8	323 ± 73
Cirrhosis	20
Untreated	5	16.01 ± 1.32a	79.6 ± 14.2	339 ± 89
Saline	5	15.87 ± 1.40a	80.1 ± 11.5	282 ± 101
EGFP	5	15.65 ± 1.18a	82.9 ± 12.9	319 ± 78
Akt	5	9.23 ± 1.51ceg	88.5 ± 17.6	289 ± 96

^aP < 0. 05 (vs Normal control);

^cP < 0. 05 (vs Untreated group);

^eP < 0. 05 (vs Saline group);

^gP < 0. 05 [vs enhanced green fluorescent protein (EGFP) group]. No difference was detected among Untreated, Saline and EGFP groups. PVP: Portal vein pressure; MAP: Mean arterial pressure; HR: Heart rate.

Table 4 NO content in liver tissue

Groups	n	NO (μmol/g)
Normal	5	2.53 ± 0. 61
Cirrhosis	20
Untreated	5	1.20 ± 0.77a
Saline	5	1.03 ± 0.87a
EGFP	5	1.15 ± 0.58a
Akt	5	2.38 ± 0.67ceg

^aP < 0.05 (vs normal control);

^cP < 0.05 (vs Untreated group);

^eP < 0.05 (vs Saline group);

^gP < 0.05 [vs enhanced green fluorescent protein (EGFP) group]. No difference was detected among Untreated, Saline and EGFP groups.