Annexin A2 silencing inhibits invasion, migration, and tumorigenic potential of hepatoma cells

Table 1 Expression level of ANXA2 mRNA in hepatocellular carcinoma cells and normal hepatic cells

Group	n	CtANXA2	Ctβ-actin	2-ΔΔCt
LO2	5	25.16 ± 0.09	20.86 ± 0.03	1.00
HepG2	5	22.14 ± 0.15	20.66 ± 0.02	7.07 ± 0.35b
SMMC-7402	5	22.87 ± 0.15	20.80 ± 0.14	4.68 ± 0.31b
SMMC-7721	5	22.21 ± 0.12	20.72 ± 0.10	7.02 ± 0.19b
MHCC97-H	5	21.85 ± 0.26	20.78 ± 0.13	9.45 ± 0.53b

^bP < 0.001 vs the LO2 group.

Table 2 Inhibition of ANXA2 mRNA expression by small hairpin in MHCC97-H cells

Group	n	CtANXA2	Ctβ-actin	2-ΔΔCt
MHCC97-H	5	21.84 ± 0.11	20.77 ± 0.16	1.00
MHCC97-H/control-shRNA	5	21.83 ± 0.21	20.72 ± 0.10	0.97 ± 0.04
MHCC97-H/ANXA2-shRNA	5	24.24 ± 0.55	20.80 ± 0.14	0.20 ± 0.05b

^bP < 0.001 vs the MHCC97-H group. shRNA: Small hairpin RNA.

Table 3 Intensity of ANXA2 expression in xenograft tumours

Group	n	ANXA2 intensity				Z
		-	+	++	+++
MHCC97-H	4	0	0	0	4
MHCC97-H/control-shRNA	4	0	0	1	3	1.000
MHCC97-H/ANXA2-shRNA	4	1	3	0	0	2.530a

^aP < 0.05 vs the MHCC97-H group. shRNA: Small hairpin RNA.