Interaction of 14-3-3σ with KCMF1 suppresses the proliferation and colony formation of human colon cancer stem cells

Table 1 Different plasmids transfected into HEK 293FT cells

Plasmid	1	2	3	4	5	6	7	8	Transfection control
	Sample 1	Negative	Sample 2	Negative	Sample 3	Negative	Negative	Positive	Positive	Negative
	KCMF1	Control-1	NQO2	Control-2	HIBADH	Control-3	Control-4
Flag-mfrP53	-	-	-	-	-	-	-	+	pEGFP	293FT
Myc-Large T	-	-	-	-	-	-	-	+
Flag-14-3-3	+	-	+	-	+	-	+	-
Myc-HIBADH	-	-	-	-	+	+	-	-
Myc-NQO2	-	-	+	+	-	-	-	-
Myc-KCMF1	+	+	-	-	-	-	-	-
VECTOR-Myc	-	-	-	-	-	-	-	-
VECTOR-Flag	-	+	-	+	-	+	-	-

KCMF1: Potassium channel modulatory factor 1; NQO2: Quinone oxidoreductase; HIBADH: Hydroxyisobutyrate dehydrogenase.

Table 2 Positive hit of the screening HeLa cDNA library

Bait	Prey library	Identical colonies	Positive clone name	Positive gene identified	NCBI accession number	Gene code match
pGB-14-3-3		19	Sigma 12	SFN	NM_006142.3	Yes	Full
			Sigma 13				Lengh
			Sigma 30
	Human HeLa		Sigma 4	HIBADH	NM_152740.3	Yes	Full
	MATCHMAKER		Sigma 15				Lengh
	cDNA library		Sigma 21	NQO2	NM_000904.3	Yes	Full
			Sigma 32				Lengh
			Sigma 23
			Sigma 33				Full
			Sigma 28	KCMF1	NM_020122.4	Yes	Lengh

KCMF1: Potassium channel modulatory factor 1; NQO2: Quinone oxidoreductase; HIBADH: Hydroxyisobutyrate dehydrogenase.