Epithelial markers of colorectal carcinogenesis in ulcerative colitis and primary sclerosing cholangitis

Table 1 Demographic features of participating patients

	n	Age (yr)	Sex (M/F)	Duration of UC (yr)	PSC	Duration after OLT (yr)	Histology	Endoscopy score (Mayo)
N	10	52.2 ± 14.09	4/6	0	No	0	0	0
UCR	7	41.57 ± 13.35	3/4	9.57 ± 1.59	No	0	0.42 ± 0.4	0.71 ± 0.45
UCA	9	45.88 ± 17.62	5/4	9.56 ± 2.41	No	0	2.7 ± 0.4	2.5 ± 0.49
PSC-UC	8	37.12 ± 6.8	5/3	8.75 ± 1.56	Yes (n = 8)	0	2.1 ± 0.59	1.12 ± 0.33
OLT	22	43.33 ± 12.11	11/11	12.4 ± 5.24	No	5.19 ± 2.61	1.4 ± 0.49	1.09 ± 0.29

N: Controls; M: Male; F: Female; UC: Ulcerative colitis; N: UCR: UC in remission; PSC: Primary sclerosing cholangitis; OLT: UC after liver transplantation for PSC; UCA: UC active disease.

Table 2 Monoclonal and polyclonal antibodies used in this study

Specificity	Origin	Company	Antigen retrieval	Dilution of antibodies
bcl-2 Oncoprotein	Mouse	DakoCytomation, Denmark	Buffer EDTA, pH 8	20 ×
p53	Mouse	DakoCytomation, Denmark	Tris/EDTA, pH 9	40 ×
COX-2	Mouse	Cayman, Michigan, United States	Citrate buffer, pH 6	40 ×

COX-2: Cyclooxygenase-2.

Table 3 Relationship between p53, cyclooxygenase-2 and primary sclerosing cholangitis n (%)

	PSC-	PSC+	Row total	COX-2-	COX-2+	Row total
p53-	43 (78.18)	0 (0)	43 (78.18)	34 (61.82)	5 (9.09)	39 (70.91)
p53+	4 (7.27)	8 (14.55)	12 (21.82)	9 (16.36)	7 (12.73)	16 (29.09)
Column total	47 (85.45)	8 (14.55)	55 (100)	43 (78.18)	12 (21.82)	55 (100)

Statistical significance (Fisher’s exact test) P < 0.001. PSC: Primary sclerosing cholangitis; COX-2: Cyclooxygenase-2.

Table 4 Comparison of primary sclerosing cholangitis-ulcerative colitis and ulcerative colitis after liver transplantation for primary sclerosing cholangitis

	p53	COX-2	bcl-2	Histology score	Endoscopy score (Mayo)
PSC-UC	↑a	↔	↔	↔	↔
OLT	↓	↔	↔	↔	↔
P value	P < 0.001	NS	NS	NS	NS

^aP < 0.05 vs PSC-UC group. COX-2: Cyclooxygenase-2; UC: Ulcerative colitis; PSC: Primary sclerosing cholangitis; OLT: UC after liver transplantation for PSC.