Effects of resistin-like molecule β over-expression on gastric cancer cells in vitro

Table 1 Primers sets used for reverse transcription polymerase chain reaction and real-time reverse transcription polymerase chain reaction

Primer set	Primers	Sequence	Product size (bp)
RELMβ	Forward	5’-ATGGGGCCGTCCTCTTGCCTCC-3’	336
	Reverse	5’-TCAGGTCAGGTGGCAGCAGCG-3’
MMP-2	Forward	5’-CCAAAACGGACAAAGAGT-3’	275
	Reverse	5’-ATCAGGTGTGTAGCCAAT-3’
MMP-9	Forward	5’-CAGAGATGCGTGGAGAGT-3’	220
	Reverse	5’-TCTTCCGAGTAGTTTTGG-3’
Ets1	Forward	5’-TTCACTAAAGAACAGCAAC-3’	205
	Reverse	5’-TGTCCCCAACAAAGTCTG-3’
VEGF	Forward	5’-GGCAGAATCATCACGAAG-3’	276
	Reverse	5’-TGTGCTGTAGGAAGCTCA-3’
GAPDH	Forward	5’- AGAAGGCTGGGGCTCATTTG-3’	258
	Reverse	5’- AGGGGCCATCCACAGTCTTC-3’

RT-PCR: Reverse transcription polymerase chain reaction; RELMβ: Resistin-like molecule β; MMP: Matrix metalloproteinase-2; Ets1: E26 oncogene homolog 1; VEGF: Vascular endothelial growth factor; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.