Analysis of TLR4 and TLR2 polymorphisms in inflammatory bowel disease in a Guangxi Zhuang population

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Dec 14, 2012 (publication date) through Nov 19, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Dec 14, 2012; 18(46): 6856-6860
Published online Dec 14, 2012. doi: 10.3748/wjg.v18.i46.6856

Table 1 Specific polymerase chain reaction primers and restriction enzymes for each single nucleotide polymorphism

Gene	Amino acid substitution	Sequence of primers( 5'→3')	Fragments (bp)	Enzymes
TLR4	Asp299Gly	F-GATTAGCATACTTAGACTACTACCTCCATG	249	NcoI
		R-GATCAACTTCTGAAAAAGCATTCCCAC
	Thr399Ile	F-GGTTGCTGTTCTCAAAGTGATTTTGGGAGAA	406	HinfI
		R-ACCTGAAGACTGGAGAGTGAGTTAAATGCT
TLR2	Arg677Trp	F-GCCTACTGGGTGGAGAACCTT
		R-CCAGTTCATACTTGCACCACTC	199	AciI
	Arg753Gln	F-CCTGGCAAGTGGATCATTGAC
		R-GGCCACTCCAGGTAGGTCTT	254	PstI

TLR: Toll-like receptor.

Citation: Chen L, Lin MJ, Zhan LL, Lv XP. Analysis of TLR4 and TLR2 polymorphisms in inflammatory bowel disease in a Guangxi Zhuang population. World J Gastroenterol 2012; 18(46): 6856-6860
URL: https://www.wjgnet.com/1007-9327/full/v18/i46/6856.htm
DOI: https://dx.doi.org/10.3748/wjg.v18.i46.6856