Brief Article
Copyright ©2012 Baishideng Publishing Group Co.
World J Gastroenterol. Nov 28, 2012; 18(44): 6481-6488
Published online Nov 28, 2012. doi: 10.3748/wjg.v18.i44.6481
Table 1 Sequence of primers and probes
NamesSequences
IL28B primers forward (9860)GTGCCTGTCGTGTACTGAAC
IL28B primers reverse (9860)CGCTGAGCACTGCCTGGGC
IL28B primers forward (9917)ATTTGTCACTGTTCCTCCT
IL28B primers reverse (9917)GCCCTAACTGATACGCTATAA
rs12979860 probe (C allele)AAAAAAAAAAAGCTCCCCGAAGGCGCGAACCAGGGTTGAAT
rs12979860 probe (T allele)AAAAAAAAAAAGCTCCCCGAAGGCGTGAACCAGGGTTGAAT
rs8099917 probe (T allele)AAAAAAAAAACTTTCTGTGAGCAATTTCACCCAAATTGGAA
rs8099917 probe (G allele)AAAAAAAAAACTTTCTGTGAGCAATGTCACCCAAATTGGAA
Table 2 Concordance of biosensor-based microarray assay with given plasmids for rs12979860 and rs8099917
BBM assayPlasmids with given genotype
Total
CCCTTT
rs12979860
CC100010
CT010010
TT0101010
Total10101030
rs8099917
TT100010
GT010010
GG001010
Total10101030
Table 3 Concordance of biosensor-based microarray assay with polymerase chain reaction sequencing of the blood samples for rs12979860 and rs8099917
BBM assayDirect sequencing
Total
CCCTTT
rs129798601
CC240024
CT311014
TT0022
Total2711240
rs80999172
TT280028
GT29011
GG0012
Total309140