Eosinophil associated genes in the inflammatory bowel disease 4 region: Correlation to inflammatory bowel disease revealed

Table 1 Clinical characteristic of study populations

	Crohn’s disease (n = 587)	Ulcerative colitis (n = 592)	Reference group (n = 300)
Female	282	269	132
Male	305	323	168
Median age1	48 (18-95)	48 (14-102)	44 (19-73)
Age at disease diagnosis2	28 (6-93)	28 (4-78)

¹Range at sampling;

²Median (range).

Table 2 Primers and probes

EPX405 (G > C):
Forward primer	5’ AAG AGA GCT GAC GTT AGT GCT TAG G 3’
Reverse primer	5’ GGT CTT GGT TAT GAC ACA CAC TGT AGT 3’
Probe 1	5’ VIC-ACG TTG CAC ACT TT 3’
Probe 2	5’ 6-FAM-CGT TCC ACA CTT TG 3’
ECP434 (G > C):
Forward primer	5’ GAG TAG ATT CCG GGT GCC TTT ACT 3’
Reverse primer	5’ CGT GGA GAA TCC CGT GGA T 3’
Probe 1	5’ VIC-AAA CTG CAG GTA TGC AGA 3’
Probe 2	5’ 6-FAM-AAA CTG CAC GTA TGC AG 3’
ECP562 (G > C):
Forward primer	5’ GGT TCC AGT TCA CCT GGA TAC C 3’
Reverse primer	5’ GGT ATG GAG ACT GAT GAG GAC AGT 3’
Probe 1	5’ VIC-TCA GCA GTC CTC ATC 3’
Probe 2	5’ FAM-TCA GCA CTC CTC ATC 3’

EPX: Eosinophil protein X; ECP: Eosinophil cationic protein.

Table 3 Genotype and allelic frequency distribution among 300 healthy individuals and the total cohorts of Crohn’s disease and ulcerative colitis

	GG-genotype (%)	GC-genotype (%)	CC-genotype (%)	G-allele (%)	C-allele (%)
Healthy
ECP562	68	29	3	83	17
ECP434	57	38	5	76	24
EPX405 M1	64	32	4	80	20
EPX405 F1	53	38	9	72	28
CD
ECP562	64	33	4	80	20
ECP434	53	40	7	73	27
EPX405 M	55	38	6	74	26
EPX405 F	58	34	8	75	25
UC
ECP562	64	33	4	80	20
ECP434	55	38	7	74	26
EPX405 M	62	31	7	78	22
EPX405 F	57	38	5	76	24

For ECP434 and ECP562 there was no difference between males and females.

¹In EPX405 there was a difference between the sexes according their genotype (P = 0.049) and allelic distribution (P = 0.021). UC: Ulcerative colitis; CD: Crohn’s disease; EPX: Eosinophil protein X; ECP: Eosinophil cationic protein; F: Female; M: Male.

Table 4 Gender specific difference

			Crohn’s disease						Ulcerative colitis
			Genotype distribution			Allelic frequency			Genotype distribution			Allelic frequency
	Age at diagnosis	Patients	EPX	ECP	ECP	EPX	ECP	ECP	EPX	ECP	ECP	EPX	ECP	ECP
	(yr)	CD/UC (n)	405	434	562	405	434	562	405	434	562	405	434	562
Female	≥ 40	80/71	NS	NS	NS	NS	0.043	0.034	NS	NS	NS	NS	NS	NS
	≥ 45	42/62	NS	0.009	0.013	NS	0.004	0.007	NS	NS	NS	NS	NS	NS
Male	≥ 40	77/85	NS	NS	NS	NS	NS	NS	0.048	0.025	0.008	0.026	0.024	0.045
	≥ 45	57/52	NS	NS	NS	NS	NS	NS	NS	0.01	0.009	NS	NS	NS

Difference (χ², P-values displayed) between the genotype distributions and allelic frequencies, related to age at diagnosis, in individuals with CD or UC compared with the healthy population. UC: Ulcerative colitis; CD: Crohn’s disease; NS: Not significant; EPX: Eosinophil protein X; ECP: Eosinophil cationic protein.

Table 5 Haplotype compositions and their frequencies in the studied population with inflammatory bowel disease

Gender	Haplotype	EPX405	ECP434	ECP562	Frequency %
Males with UC	1	GG	GG	GG	48
	13	GC	GC	GG	5
	14	GC	GC	GC	20
	27	CC	CC	CC	3
Females with CD	1	GG	GG	GG	46
	4	GG	GC	GG	6
	13	GC	GC	GG	5
	27	CC	CC	CC	5

UC: Ulcerative colitis; CD: Crohn’s disease; EPX: Eosinophil protein X; ECP: Eosinophil cationic protein.