Correlation between mitochondrial TRAP-1 expression and lymph node metastasis in colorectal cancer

Table 1 Relationship between tumor necrosis factor receptor-associated protein-1 expression and clinicopathological factors in colorectal cancer

Clinicopathological factors	n	TRAP-1 expression		P value1
		Negative	Positive
Sex
Male	46	16	30	0.225
Female	50	23	30
Age (yr)
≤ 60	66	30	36	0.486
> 60	30	10	20
Tumor size (cm)
≤ 5	70	30	40	0.065
> 5	26	11	15
Tumor location
Colon	31	11	20	0.915
Rectum	65	32	33
Tumor differentiation2
I-II	80	45	35	0.212
III-IV	16	6	10
Tumor status2
T1-2	34	14	20	0.512
T3-4	62	31	31
Lymph node metastasis2
N0	45	35	10	< 0.001
N1-2	51	5	46
TNM stage2
I-II	41	30	11	< 0.001
III-IV	55	6	49

¹Statistical analysis was performed with χ² test;

²Grading of differentiation status and tumor node metastasis (TNM) classification for colorectal cancer were based on the American Joint Committee on Cancer: Cancer Staging Manual (7th edition, 2009). The tumors were classified into two groups: Well differentiated (grades I and II) and poorly differentiated (grades III and IV).

Table 2 Univariate and multivariate analyses of recurrence and survival (Cox regression)

Variables	Recurrence		Survival
	HR (95%CI)	P value	HR (95%CI)	P value
Univariate analysis
Sex
Male/female	0.813 (0.479-1.518)	0.604	0.829 (0.424-1.618)	0.662
Age (yr)
≤ 60/> 60	1.506 (0.804-2.712)	0.163	1.822 (0.947-3.528)	0.065
Tumor size (cm)
≤ 5/> 5	0.876 (0.163-0.665)	0.687	0.880 (0.438-1.723)	0.704
Tumor location
Colon/rectum	0.812 (0.445-1.423)	0.518	0.904 (0.476-1.734)	0.778
Tumor differentiation
I-II/III-IV	1.212 (0.654-2.308)	0.501	1.151 (0.576-2.358)	0.65
Tumor status
T1-2/T3-4	0.866 (0.475-1.618)	0.687	1.020 (0.504-2.028)	0.904
Lymph node metastasis
N0/N1-2	2.707 (1.502-4.912)	0.001	2.812 (1.413-5.509)	0.002
TNM stage
I-II/III-IV	3.554 (1.932-6.526)	< 0.001	3.385 (1.677-6.843)	< 0.001
TRAP-1 expression
Negative/positive	3.657 (1.919-6.957)	< 0.001	4.145 (1.913-8.712)	< 0.001
Multivariate analysis
LNM
N0/N1-2	0.210 (0.051-0.758)	0.018	0.196 (0.041-0.852)	0.028
TNM stage
I-II/III-IV	8.905 (2.072-38.190)	0.003	9.037 (1.703-48.105)	0.010
TRAP-1 expression
Negative/positive	2.445 (1.065-5.712)	0.017	2.867 (1.113-7.36)	0.028

HR: Hazard ratio; CI: Confidence interval; LNM: Lymph node metastasis; TNM: Tumor node metastasis; TRAP-1: Tumor necrosis factor receptor-associated protein-1.