Inducible nitric oxide synthetase genotype and Helicobacter pylori infection affect gastric cancer risk

Table 1 Patient demographics and risk behavior

	Gastric cancer	Control	P-value
	n = 159 (%)	n = 170 (%)
Age (yr)	61.32 ± 12.5	58.86 ± 14.2	0.098
Sex (male/female)	84/75	98/72	0.367
Marital status
Single	7 (4.4)	3 (1.8)
Married	149 (93.7)	166 (97.6)	0.002
Divorced	3 (1.9)	1 (0.6)
Occupation
Unemployed	4 (2.5)	1 (0.6)
Employee	24 (15.1)	35 (20.5)	0.001
Housewife	46 (28.9)	74 (43.5)
Others	85 (53.5)	60 (5.4)
Smoker	33 (20.8)	22 (12.9)	0.015
Hot beverage	76 (47.8)	38 (22.4)	< 0.0001
Salty fish	15 (9.4)	14 (8.2)	0.09
Fast food	29 (18.2)	39 (22.9)	0.037
Pickles	88 (55.3)	97 (57.1)	0.62
H. pylori positivity	94 (59.1)	77 (45.3)	0.015
Positive family history	34 (21.4)	16 (9.4)	0.0034

H. pylori: Helicobacter pylori.

Table 2 Allele and genotype frequency distribution of C150T polymorphism of the inducible nitric oxide synthetase gene in patients with gastric cancer and controls n (%)

iNOS C150T	Gastric cancer n = 159	Controlsn = 170	Odds ratio	95%CI	P-value
CC	89 (56)	92 (54.1)	1.001
CT	59 (37.1)	72 (42.4)	0.86	0.54-1.35	0.5
TT	11 (6.9)	6 (3.5)	1.917	0.68-5.41	0.21
C allele	237 (74.5)	256 (75.3)	1.001
T	81 (25.5)	84 (24.7)	1.05	0.74-1.49	0.78
TT + CT	70 (44)	78 (45.9)	0.94	0.61-1.45	0.77
HWE	0.796	0.07

¹The first allele or genotype is the reference for statistical analysis. iNOS: Inducible nitric oxide synthetase; CC: Homozygous CC genotype; CT: Heterozygous CT genotype; TT: Homozygous TT genotype; HWE: Hardy Weinberg Equilibrium; CI: Confidence interval.

Table 3 Allele and genotype frequency distribution of C150T polymorphism of the inducible nitric oxide synthetase gene in Helicobacter pylori³n (%)

	H. pylori+	H. pylori-	OR2	95%CI	P-value
iNOS C150T GC patients	n= 94	n= 65
CC	44 (46.8)	44 (67.7)	1.001
CT	40 (42.6)	19 (29.2)	2.1	1.06-4.19	0.03
TT	10 (10.6)	2 (3.1)	5	1.03-24.14	0.029
C	128 (68.1)	107 (82.3)	1.001
T	60 (31.9)	23 (17.7)	2.2	1.26-3.76	0.004
TT + CT	50 (53.2)	21 (32.3)	2.4	1.23-4.6	0.01
Controls	n = 77	n = 93
CC	40 (51.9)	52 (55.9)	1.001
CT	36 (46.8)	36 (38.7)	1.3	0.7-2.41	0.41
TT	1 (1.3)	5 (5.4)	0.26	0.03-2.31	0.197
C	116 (75.3)	140 (75.3)	1.001
T	38 (24.7)	46 (24.7)	0.99	0.61-1.64	0.99
TT + CT	37 (48.05)	41 (44.1)	1.2	0.64-2.15	0.6

¹The first allele or genotype is the reference for statistical analysis;

²Adjusted for age and gender; ³Infected or non-infected patients with and without GC. GC: Gastric cancer; iNOS: Inducible nitric oxide synthetase; CC: Homozygous CC genotype; CT: Heterozygous CT genotype; TT: Homozygous TT genotype; C: Number of individual C alleles in the group; T: Number of individual T alleles in the group; HWE: Hardy Weinberg Equilibrium; OR: Odds ratio; CI: Confidence interval; H. pylori: Helicobacter pylori.