Expression of the P2X2 receptor in different classes of ileum myenteric neurons in the female obese ob/ob mouse

Table 1 Characteristics of primary and secondary antibodies

Antigen	Host	Dilution	Code and reference
P2X2 receptor	Rabbit	1:120	AB5244, Chemicon
Nitric oxide synthase	Sheep	1:2000	H205 (Williamson et al[31], 1996)
Choline acetyltransferase	Goat	1:50	Chemicon
Calretinin	Goat	1:100	CG1 (Swant)
Secondary antibodies
Donkey anti-rabbit IgG Alexa 488	Donkey	1:500	Molecular probes
Donkey anti-sheep IgG Alexa 594	Donkey	1:100	Molecular probes

Table 2 Body weight, small intestine area and glucose levels in female control (n = 4) and ob/ob mice (n = 4)

	Control group	Obese group
Body weight (g)	25.62 ± 0.62	65.52 ± 1.08a
Small intestine area (cm2)	26.6 ± 2.8	34.5 ± 3.21a
Glucose (mg/dL)	195.75 ± 58.09	322.2 ± 99.2a

Student’s t-tests for multiple comparisons.

^aP < 0.05 vs control.

Table 3 Results of the quantitative analysis of double labeling studies of immunoreactivity against the P2X₂ receptor, neuronal nitric oxide synthase, choline acetyltransferase, and calretinin in the myenteric plexus of the ileum from control and ob/ob female mice (n = 4, mean ± SE) (%)

	Control group	Obese group
nNOS-IR+/P2X2-IR+	99.5 ± 0.6	99 ± 1.0
ChAT-IR+/P2X2-IR+	100	100
CalR-IR+/ P2X2-IR+	100	100
P2X2-IR+/nNOS-IR+	19.5 ± 3.6	19.5 ± 3.6
P2X2-IR+/ChAT-IR+	23.6 ± 4.2	24.6 ± 4.37
P2X2-IR+/CalR-IR+	23.7 ± 3.75	21.6 ± 1.20

nNOS: Neuronal nitric oxide synthase; ChAT: Choline acetyltransferase; CalR: Calretinin; IR: Immunoreactivity.