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©2012 Baishideng Publishing Group Co.
World J Gastroenterol. Aug 28, 2012; 18(32): 4257-4269
Published online Aug 28, 2012. doi: 10.3748/wjg.v18.i32.4257
Published online Aug 28, 2012. doi: 10.3748/wjg.v18.i32.4257
No. | Groups (n = 5 per group) | iv injection | Drug administration (ip) |
1 | Naïve | Non | Non |
2 | Vehicle/PBS | Vehicle | 0.1 mol/L PBS |
3 | Vehicle/BQ-123 or BQ-788 | Vehicle | 300 μmol/L |
4 | DBTC/PBS | DBTC | 0.1 mol/L PBS |
5 | DBTC/BQ-123 or BQ-788 | DBTC | 33 μmol/L |
6 | DBTC/BQ-123 or BQ-788 | DBTC | 100 μmol/L |
7 | DBTC/BQ-123 or BQ-788 | DBTC | 300 μmol/L |
Group | Regulated genes | Abbreviation | Fold |
Up-regulated genes1 | |||
1 | B-lymphocyte surface antigen | 7.3 | |
1 | Endothelin-1 | ET-1 | 3.2 |
1 | Eosinophil chemotactic protein | ECP | 11 |
1 | T-cell surface glycoprotein CD5 | 2.6 | |
1 | Tumor necrosis factor | TNF-α | 4 |
2 | Laminin α 5 | LAMA5 | 2.5 |
2 | Matrix gla protein | MGP | 7.4 |
2 | Integrin α | 27 | |
3 | Epithelial-cadherin | E-CADHERIN | 3.1 |
3 | Transmembrane ER-resident protein, type I | IRE1b | 23.5 |
4 | ATP binding cassette | ABC-transporter | 23 |
4 | C-C chemokine receptor type 8 | C-C CKR-8, GPR-CY6 | 3.4 |
4 | GABAA receptor | GABAA R | 14.3 |
4 | Glutamate receptor ionotropic, AMPA 1 | GluR-1 | 9.1 |
4 | Putative G protein-coupled receptor | GPR68 (OGR-1) | 3.1 |
5 | Calcium binding protein | CHP | 2.6 |
5 | Caveolin-1 | CAV1 | 3.8 |
5 | Iron response regulator | IRR | 2.8 |
5 | LDL receptor-related protein 6 | LDL-R6 | 3.6 |
5 | Insulin-like growth factor binding complex acid label chain precursor | ALS | 2.7 |
5 | Proto-oncogene tyrosine-protein kinase FES/FPS | C-FES | 8 |
5 | RAS GTPase-activating-like protein | RAN | 3.9 |
5 | Retinoic acid-inducible gene-1 | RAIG1 | 6.4 |
5 | Transforming growth factor-b | TGFb | 2.8 |
5 | Guanine nucleotide binding protein | G (Y) α-11 | 4.7 |
6 | Nuclear factor of activated T cells | NF-ATc | 3.5 |
6 | Signal transducer and activator of transcription 5B | STAT5b | 3 |
6 | Transcription factor | TF | 11.8 |
7 | Glutathione peroxidase gastrointestinal | GSHPX_GI | 2.5 |
7 | Extracellular superoxide dismutase | EC-SOD | 2.9 |
7 | Heat shock related 70 kD proteins2 | 2.3 | |
7 | NADH dehydrogenase (ubiquinone) | 2.3 | |
7 | Serum amine oxidase | SAO | 2.2 |
8 | ACE-related carboxypeptidase | ACE2 | 3.6 |
8 | Aminopeptidase N | APN | 2.3 |
8 | ATP-dependent RNA helicase P54 | DDX6 | 2 |
8 | a enolase | 2 | |
8 | a-Tryptase | TRYPTASE 1 | 2.6 |
8 | cAMP cAMP-inhibited cGMP 3’5’-cyclic phosphodiesterase | 9.4 | |
8 | Cholecystokinin type A receptor | CCK-AR | 2.8 |
8 | Dopamine β-hydroxylase | DBH | 5.4 |
8 | GAMMA-glutamyltransferase 1 | Gamma-GT | 5.6 |
8 | (GDP-D-MANNOSE dehydratase | GMD | 3.5 |
8 | Glycolipid transfer protein | GLTP | 2.6 |
8 | Lipase | 3.8 | |
8 | Lysophosphatidic acid acyltransferase-b | LPAAT- b | 3.4 |
8 | Malonyl-CoA decarboxylase | MLYCD | 4.8 |
8 | NTPDase 3; CD39L 3 | ENTPD3 | 7.2 |
8 | Pancreatic isozyme | Glucokinase | 3.4 |
8 | Phosphate regulating neutral endopeptidase | NEP | 11 |
8 | Putative NAD (P)-dependent cholesterol dehydrogenase | MDH | 3 |
8 | Red cell phosphatase1, isozyme F | ACP1 | 2.3 |
Down-regulated genes1 | |||
1 | Insulin precursor | 2.8 | |
1 | IG GAMMA-3 chain C region | HDC | 2 |
1 | Interlaken 1 receptor 1 precursor | IL-1R, IL-1Rα | 3.8 |
1 | Growth regulated protein (neutrophil-activating protein 3) | NAP-3 | 2.3 |
1 | Natural killer cells antigen CD94 | KP43 | 4.2 |
1 | Monocyte chemotactic protein 1 | 4.3 | |
1 | T-cell surface glycoprotein CD3 e chain precursor | 5.2 | |
1 | Heparin-binding growth factor 1 | 7.3 | |
2 | EGF-containing fibulin-like extracellular matrix protein 1 | FIBL-3 | 2.1 |
2 | Homocontig12 | 2.3 | |
2 | MT4-MMP | 2 | |
3 | Glocosyl structure | 2 | |
3 | Microtubule-associated protein 2 | MAP2C | 2.1 |
3 | Mucosal addressin cell adhesion molecule-1 | 2.1 | |
3 | Peripheral myelin protein 22 | PMP-22 | 3.8 |
4 | Growth factor regulated channel 5 | GRC5 | 2.1 |
4 | Growth hormone secretagogue receptor type 1 | 2 | |
4 | a platelet-derived growth factor receptor (CD104A antigen) | PDGFRa | 2.1 |
4 | Glucose transporter type 5, small intestine (fructose transporter) | 2.2 | |
4 | Dopamine receptor | 2.3 | |
4 | C-C chemokine receptor type 9 | CCR-9 | 2.4 |
4 | Monocarboxylate-transporter 3 | MCT 3 | 2.9 |
4 | Orphan receptor | GRF | 3.6 |
4 | G protein-coupled receptor | GRP1 | 3.6 |
4 | Neuropeptide Y Receptor Type 4 | NPY4-R | 10.6 |
4 | b 3 Adrenergic Receptor | 2.5 | |
5 | G25K GTP-binding protein, placental isoform (GP) | 2 | |
5 | MAD 2 | 2 | |
5 | Ribosomal S6 protein kinase | 2 | |
5 | Metalloproteinase Inhibitor 3 | TIMP-3 | 2.2 |
5 | GTPase RhoD | RhoD | 2.4 |
5 | Ankyrins | 2.7 | |
5 | Putative RHO/RAC effector protein | 4.2 | |
5 | Protein farnesyltransferase β subunit (RAS Proteins prenyltransferase) | FTASE-β | 2.4 |
6 | GTP-binding protein | RHEB | 2.2 |
6 | GTP-binding protein 1 | GP-1 | 2.3 |
6 | hLHX 6 1α | 2 | |
6 | AMP-binding protein | 2.7 | |
7 | Thyroid peroxidase | TPO | 2.1 |
8 | Ubiquitin-conjugating enzyme E2 (ubiquitin-protein ligase) | UBE2 | 2 |
8 | Steryl-sulfatase (steroid sulfatase) (steryl-sulfate sulfhydrolase) | ASC | 2 |
8 | Histone deacetylase 2 | HD2 | 2.1 |
8 | Corticosteroid 11-β-dehydrogenase, isozyme 2 | 4.3 | |
8 | Hydroxymethylglutaryl-CoA synthase | 4.4 | |
8 | ATP synthase oligomycin sensitivity conferral protein | OSCP | 4.9 |
8 | Indoleamine 2,3-dioxygenase | IDO | 2.1 |
8 | Lysozyme | 2.2 | |
8 | ubiquitin C-terminal hydrolase | UCH | 2.3 |
8 | Tyrosine aminotransferase,L-tyrosine: 2-oxoglutarate Aminotransferase | TAT | 2.7 |
8 | Corticosteroid 11-β-dehydrogenase, isozyme 2 | 4.3 | |
8 | Hydroxymethylglutaryl-CoA synthase | 4.4 | |
8 | ATP synthase oligomycin sensitivity conferral protein | OSCP | 4.9 |
8 | Glycogen phosphorylase | 8.5 |
Groups | G1 | G2 | G3 | G4 | G5 | G6 | G7 | G8 | G9 | % | Total |
Up | 17 | 17 | 8 | 30 | 46 | 23 | 9 | 67 | 44 | 81.3 | 261 |
Down | 7 | 3 | 5 | 11 | 12 | 4 | 1 | 14 | 3 | 18.7 | 60 |
Sum | 24 | 20 | 13 | 41 | 58 | 27 | 10 | 81 | 47 | 321 | |
Total% | 7.5 | 6.2 | 4.1 | 12.8 | 18.1 | 8.4 | 3.1 | 25.2 | 14.6 | 100 | |
Sum | 8.7 | 4.62 | 5.86 | 13.98 | 16.56 | 6.24 | 1.86 | 20.52 | 7.26 | 118.7 | 334 |
- Citation: Oz HS, Lu Y, Vera-Portocarrero LP, Ge P, Silos-Santiago A, Westlund KN. Gene expression profiling and endothelin in acute experimental pancreatitis. World J Gastroenterol 2012; 18(32): 4257-4269
- URL: https://www.wjgnet.com/1007-9327/full/v18/i32/4257.htm
- DOI: https://dx.doi.org/10.3748/wjg.v18.i32.4257