Original Article
Copyright ©2012 Baishideng Publishing Group Co.
World J Gastroenterol. Aug 28, 2012; 18(32): 4257-4269
Published online Aug 28, 2012. doi: 10.3748/wjg.v18.i32.4257
Table 1 Experimental design
No.Groups (n = 5 per group)iv injectionDrug administration (ip)
1NaïveNonNon
2Vehicle/PBSVehicle0.1 mol/L PBS
3Vehicle/BQ-123 or BQ-788Vehicle300 μmol/L
4DBTC/PBSDBTC0.1 mol/L PBS
5DBTC/BQ-123 or BQ-788DBTC33 μmol/L
6DBTC/BQ-123 or BQ-788DBTC100 μmol/L
7DBTC/BQ-123 or BQ-788DBTC300 μmol/L
Table 2 Up-regulated and down-regulated genes expressed1
GroupRegulated genesAbbreviationFold
Up-regulated genes1
1B-lymphocyte surface antigen7.3
1Endothelin-1ET-13.2
1Eosinophil chemotactic proteinECP11
1T-cell surface glycoprotein CD52.6
1Tumor necrosis factorTNF-α4
2Laminin α 5LAMA52.5
2Matrix gla proteinMGP7.4
2Integrin α27
3Epithelial-cadherinE-CADHERIN3.1
3Transmembrane ER-resident protein, type IIRE1b23.5
4ATP binding cassetteABC-transporter23
4C-C chemokine receptor type 8C-C CKR-8, GPR-CY63.4
4GABAA receptorGABAA R14.3
4Glutamate receptor ionotropic, AMPA 1GluR-19.1
4Putative G protein-coupled receptorGPR68 (OGR-1)3.1
5Calcium binding proteinCHP2.6
5Caveolin-1CAV13.8
5Iron response regulatorIRR2.8
5LDL receptor-related protein 6LDL-R63.6
5Insulin-like growth factor binding complex acid label chain precursorALS2.7
5Proto-oncogene tyrosine-protein kinase FES/FPSC-FES8
5RAS GTPase-activating-like proteinRAN3.9
5Retinoic acid-inducible gene-1RAIG16.4
5Transforming growth factor-bTGFb2.8
5Guanine nucleotide binding proteinG (Y) α-114.7
6Nuclear factor of activated T cellsNF-ATc3.5
6Signal transducer and activator of transcription 5BSTAT5b3
6Transcription factorTF11.8
7Glutathione peroxidase gastrointestinalGSHPX_GI2.5
7Extracellular superoxide dismutaseEC-SOD2.9
7Heat shock related 70 kD proteins22.3
7NADH dehydrogenase (ubiquinone)2.3
7Serum amine oxidaseSAO2.2
8ACE-related carboxypeptidaseACE23.6
8Aminopeptidase NAPN2.3
8ATP-dependent RNA helicase P54DDX62
8a enolase2
8a-TryptaseTRYPTASE 12.6
8cAMP cAMP-inhibited cGMP 3’5’-cyclic phosphodiesterase9.4
8Cholecystokinin type A receptorCCK-AR2.8
8Dopamine β-hydroxylaseDBH5.4
8GAMMA-glutamyltransferase 1Gamma-GT5.6
8(GDP-D-MANNOSE dehydrataseGMD3.5
8Glycolipid transfer proteinGLTP2.6
8Lipase3.8
8Lysophosphatidic acid acyltransferase-bLPAAT- b3.4
8Malonyl-CoA decarboxylaseMLYCD4.8
8NTPDase 3; CD39L 3ENTPD37.2
8Pancreatic isozymeGlucokinase3.4
8Phosphate regulating neutral endopeptidaseNEP11
8Putative NAD (P)-dependent cholesterol dehydrogenaseMDH3
8Red cell phosphatase1, isozyme FACP12.3
Down-regulated genes1
1Insulin precursor2.8
1IG GAMMA-3 chain C regionHDC2
1Interlaken 1 receptor 1 precursorIL-1R, IL-1Rα3.8
1Growth regulated protein (neutrophil-activating protein 3)NAP-32.3
1Natural killer cells antigen CD94KP434.2
1Monocyte chemotactic protein 14.3
1T-cell surface glycoprotein CD3 e chain precursor5.2
1Heparin-binding growth factor 17.3
2EGF-containing fibulin-like extracellular matrix protein 1FIBL-32.1
2Homocontig122.3
2MT4-MMP2
3Glocosyl structure2
3Microtubule-associated protein 2MAP2C2.1
3Mucosal addressin cell adhesion molecule-12.1
3Peripheral myelin protein 22PMP-223.8
4Growth factor regulated channel 5GRC52.1
4Growth hormone secretagogue receptor type 12
4a platelet-derived growth factor receptor (CD104A antigen)PDGFRa2.1
4Glucose transporter type 5, small intestine (fructose transporter)2.2
4Dopamine receptor2.3
4C-C chemokine receptor type 9CCR-92.4
4Monocarboxylate-transporter 3MCT 32.9
4Orphan receptorGRF3.6
4G protein-coupled receptorGRP13.6
4Neuropeptide Y Receptor Type 4NPY4-R10.6
4b 3 Adrenergic Receptor2.5
5G25K GTP-binding protein, placental isoform (GP)2
5MAD 22
5Ribosomal S6 protein kinase2
5Metalloproteinase Inhibitor 3TIMP-32.2
5GTPase RhoDRhoD2.4
5Ankyrins2.7
5Putative RHO/RAC effector protein4.2
5Protein farnesyltransferase β subunit (RAS Proteins prenyltransferase)FTASE-β2.4
6GTP-binding proteinRHEB2.2
6GTP-binding protein 1GP-12.3
6hLHX 6 1α2
6AMP-binding protein2.7
7Thyroid peroxidaseTPO2.1
8Ubiquitin-conjugating enzyme E2 (ubiquitin-protein ligase)UBE22
8Steryl-sulfatase (steroid sulfatase) (steryl-sulfate sulfhydrolase)ASC2
8Histone deacetylase 2HD22.1
8Corticosteroid 11-β-dehydrogenase, isozyme 24.3
8Hydroxymethylglutaryl-CoA synthase4.4
8ATP synthase oligomycin sensitivity conferral proteinOSCP4.9
8Indoleamine 2,3-dioxygenaseIDO2.1
8Lysozyme2.2
8ubiquitin C-terminal hydrolaseUCH2.3
8Tyrosine aminotransferase,L-tyrosine: 2-oxoglutarate AminotransferaseTAT2.7
8Corticosteroid 11-β-dehydrogenase, isozyme 24.3
8Hydroxymethylglutaryl-CoA synthase4.4
8ATP synthase oligomycin sensitivity conferral proteinOSCP4.9
8Glycogen phosphorylase8.5
Table 3 Modified genes were classified into 9 different groups according to their functions, while some genes’ functionality may overlap1
GroupsG1G2G3G4G5G6G7G8G9%Total
Up171783046239674481.3261
Down73511124114318.760
Sum242013415827108147321
Total% 7.5  6.24.112.818.18.4  3.1  25.214.6100
Sum 8.7  4.625.8613.9816.566.24  1.86  20.527.26118.7334