Matrix metalloproteinases in the restorative proctocolectomy pouch of pediatric ulcerative colitis

Table 1 Patient characteristics

Patient characteristics	Median (range)
Number of patients (male)	28 (9)
Age (yr); median (range)
At diagnosis	12 (1-15)
At operation	13 (2-19)
At the time of study	25 (8-41)
Duration from surgery to study	13 (4-22)
Clinical inflammation markers; mean (range)
Fecal calprotectin (μg/g)	371 (12-2859)
ESR (mm/h)	14 (2-69)
CRP (mg/L)	3 (0-19)

ESR: Erythrocyte sedimentation rate; CRP: C-reactive protein.

Table 2 Medications at the time of study

Intestinal inflammation	n
5-ASA	1
5-ASA + antibiotics	1
Antibiotics	5
Bowel function
To decrease bowel motility	10
To increase bulk mass	1
Analgesics	1
Lactic acid bacteria	1
Other chronic illnesses
Cholangitis	1
Mood stabilizing agents	2
Antibiotics for acne	1
Gastro-esophageal reflux	1
Cardiac arrythmias	1
Infertility	1
Asthma	1
Hyperthyroidism	1

5-ASA: 5-Aminosalicylic acid formulations.

Table 3 Expression profiles of matrix metalloproteinases and tissue inhibitor of matrix metalloproteinase in ulcerative colitis samples

	Number of positive samples	mean±SEM1
MMP-3
Epithelium	22	0.93 ± 0.114
Stroma	20	1.25 ± 0.183
MMP-7
Epithelium	28	1.36 ± 0.092
Stroma	27	1.89 ± 0.149
MMP-8
Epithelium	0	0.00 ± 0.000
Stroma	1	0.04 ± 0.036
MMP-9
Epithelium	0	0.00 ± 0.000
Stroma	9	0.32 ± 0.090
MMP-12
Epithelium	20	0.75 ± 0.098
Stroma	24	1.46 ± 0.141
MMP-26
Epithelium	0	0.00 ± 0.000
Stroma	3	0.11 ± 0.060
TIMP-1
Epithelium	0	0.00 ± 0.000
Stroma	0	0.00 ± 0.000
TIMP-2
Epithelium	23	0.89 ± 0.094
Stroma	23	1.75 ± 0.203
TIMP-3
Epithelium	23	1.04 ± 0.120
Stroma	28	2.39 ± 0.130
Histology
Neutrophils		0.85 ± 0.154
Lymphocytes		1.07 ± 0.185
Eosinophils		1.04 ± 0.167
Villus atrophy		1.19 ± 0.227
Grade		1.95 ± 0.203

¹mean calculated from values: 0 = less than 20 positive cells; 1 = 20-50 positive cells; 2 = 50-200 positive cells; 3 = over 200 positive cells; Grade: Grade of inflammation; MMPs: Matrix metalloproteinases; TIMP: Tissue inhibitor of matrix metalloproteinase.

Table 4 Matrix metalloproteinases and tissue inhibitor of matrix metalloproteinase that correlated with inflammation indicators

Inflammation indicators	Correlation coefficients values
	MMP-3	MMP-7	MMP-12		TIMP-2
	Epithelium	Epithelium	Epithelium	Stroma	Epithelium
Fecal calprotectin	NS	NS	-0.350	NS	NS
C-reactive protein	NS	NS	NS	-0.422	NS
Grade of inflammation	-0.614	-0.472	NS	NS	-0.420

NS: Not significant; MMPs: Matrix metalloproteinases; TIMP: Tissue inhibitor of matrix metalloproteinase. Correlations were calculated using Spearman’s correlation test.