Lactobacillus plantarum B7 inhibits Helicobacter pylori growth and attenuates gastric inflammation

Table 1 Inhibition zone diameters (mm) of all Lactobacillus plantarum B7 supernatant concentrations at intact pH 4 and adjusted pH 7 (mean ± SD) (n = 2)

Concentration of L. plantarum	Diameters of inhibition zone (mm)
B7 supernatant	Intact pH 4	Adjusted pH 7
MRS (negative control)	0	0
1×	0	0
5×	8.5 ± 0.7	6.5 ± 0.7
10×	13 ± 0	11 ± 1.4

L. plantarum: Lactobacillus plantarum; MRS: Man-Rogosa-Sharpe.

Table 2 Summary of the scores for bacterial colonization levels and gastric inflammation in all groups

Group	Number	Level of H. pylori colonization1				Gastric inflammation2
		0	1	2	3	0	1	2	3
Control group	8	8	-	-	-	8	-	-	-
H. pylori infected group	8	1	5	2	-	-	3	5	-
L. plantarum B7 106 CFUs/mL treated group	8	4	4	-	-	-	8	-	-
L. plantarum B7 1010 CFUs/mL treated group	8	3	5	-	-	-	8	-	-

¹The stomach samples were evaluated for Helicobacter pylori (H. pylori) colonization by the pathologist using the following scoring system. Score 0: No bacteria detected; Score 1: Mild colonization in some gastric crypts; Score 2: Mild colonization in most gastric crypts; Score 3: Moderate colonization in all gastric crypts.

²The gastric inflammation level was estimated and scored by the pathologist following the updated Sydney System[16]. The infiltration of polymorphonuclear leucocytes in the gastric mucosa defining the inflammatory scores was recorded. Scores from 0 to 3 represented normal, mild, moderate and marked histopathology changes, respectively. L. plantarum: Lactobacillus plantarum.